70 research outputs found

    Separating Instability from Aggregation Propensity in ÎłS-Crystallin Variants

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    AbstractMolecular dynamics (MD) simulations, circular dichroism (CD), and dynamic light scattering (DLS) measurements were used to investigate the aggregation propensity of the eye-lens protein ÎłS-crystallin. The wild-type protein was investigated along with the cataract-related G18V variant and the symmetry-related G106V variant. The MD simulations suggest that local sequence differences result in dramatic differences in dynamics and hydration between these two apparently similar point mutations. This finding is supported by the experimental measurements, which show that although both variants appear to be mostly folded at room temperature, both display increased aggregation propensity. Although the disease-related G18V variant is not the most strongly destabilized, it aggregates more readily than either the wild-type or the G106V variant. These results indicate that ÎłS-crystallin provides an excellent model system for investigating the role of dynamics and hydration in aggregation by locally unfolded proteins

    Endodontic Periapical Disease: A Virus-Based Perspective

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    There is a need for updating the current treatment modalities for periapical lesions of endodontic origin to reflect the contributions and potential morbidity from herpesvirus infection. Herpesviruses, particularly human cytomegalovirus and Epstein-Barr virus, play an important role in the symptomatic periapical lesion. Indeed, periapical lesions may remain stable and silent until they become symptomatic, possibly triggered by the activation of latent herpesviruses within the lesion itself. Given the potential for grave systemic conditions associated with herpesvirus infection, early detection and treatment decisions for periapical lesions by the dentist may positively affect a patient’s overall health; especially for the immunocompromised patient

    Application of X-ray microtomography to discrete element method simulations of agglomerate breakage

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