408 research outputs found

    A Hybrid Observer for a Distributed Linear System with a Changing Neighbor Graph

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    A hybrid observer is described for estimating the state of an m>0m>0 channel, nn-dimensional, continuous-time, distributed linear system of the form x˙=Ax,  yi=Cix,  i∈{1,2,…,m}\dot{x} = Ax,\;y_i = C_ix,\;i\in\{1,2,\ldots, m\}. The system's state xx is simultaneously estimated by mm agents assuming each agent ii senses yiy_i and receives appropriately defined data from each of its current neighbors. Neighbor relations are characterized by a time-varying directed graph N(t)\mathbb{N}(t) whose vertices correspond to agents and whose arcs depict neighbor relations. Agent ii updates its estimate xix_i of xx at "event times" t1,t2,…t_1,t_2,\ldots using a local observer and a local parameter estimator. The local observer is a continuous time linear system whose input is yiy_i and whose output wiw_i is an asymptotically correct estimate of LixL_ix where LiL_i a matrix with kernel equaling the unobservable space of (Ci,A)(C_i,A). The local parameter estimator is a recursive algorithm designed to estimate, prior to each event time tjt_j, a constant parameter pjp_j which satisfies the linear equations wk(tj−τ)=Lkpj+μk(tj−τ),  k∈{1,2,…,m}w_k(t_j-\tau) = L_kp_j+\mu_k(t_j-\tau),\;k\in\{1,2,\ldots,m\}, where τ\tau is a small positive constant and μk\mu_k is the state estimation error of local observer kk. Agent ii accomplishes this by iterating its parameter estimator state ziz_i, qq times within the interval [tj−τ,tj)[t_j-\tau, t_j), and by making use of the state of each of its neighbors' parameter estimators at each iteration. The updated value of xix_i at event time tjt_j is then xi(tj)=eAτzi(q)x_i(t_j) = e^{A\tau}z_i(q). Subject to the assumptions that (i) the neighbor graph N(t)\mathbb{N}(t) is strongly connected for all time, (ii) the system whose state is to be estimated is jointly observable, (iii) qq is sufficiently large, it is shown that each estimate xix_i converges to xx exponentially fast as t→∞t\rightarrow \infty at a rate which can be controlled.Comment: 7 pages, the 56th IEEE Conference on Decision and Contro

    Attitudes and perceptions about ecological resources and hazards of people living around the Savannah River Site

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    Although considerable attention is devoted to environmental monitoring and assessment with respect to both pollutants and the status of particular plant or animal populations, less attention is devoted to assessing people\u27s attitudes about the relative importance of ecological resources. In this paper we examine the attitudes and perceptions about ecological resources of people living around the Department of Energy\u27s Savannah River Site (SRS), in South Carolina. Our overall hypothesis is that people who are directly affected by the possible outcomes and consequences of a particular hazard (i.e., those people employed at SRS) will undervalue the risks and overvalue the potential benefits from future land uses that favour continued site activity, compared to people who live near but are not employed at SRS. We interviewed 286 people attending the Aiken Trials horse show on 14 March 1997. There were few gender differences, although men hunted and fished more than women, women ranked three environmental concerns as more severe than did men, and women were more concerned about the effect of SRS on property values. Maintenance of SRS as a National Environmental Research Park ranked first as a future land use; nuclear production ranked second, followed by hunting and hiking. Only residential development ranked very low as a future land use. There were many differences as a function of employment history at SRS: (1) people who work at SRS think that the federal government should spend funds to clean up all nuclear facilities, and they think less money should be spent on other environmental problems than did non-employees, (2) people who work at SRS ranked continued current uses of SRS higher than did people who never worked at SRS, and (3) people who work at SRS are less concerned about the storage of nuclear material or accidents at the site than are people who never worked at the site

    Attitudes and perceptions about ecological resources and hazards of people living around the Savannah River Site

    Get PDF
    Although considerable attention is devoted to environmental monitoring and assessment with respect to both pollutants and the status of particular plant or animal populations, less attention is devoted to assessing people\u27s attitudes about the relative importance of ecological resources. In this paper we examine the attitudes and perceptions about ecological resources of people living around the Department of Energy\u27s Savannah River Site (SRS), in South Carolina. Our overall hypothesis is that people who are directly affected by the possible outcomes and consequences of a particular hazard (i.e., those people employed at SRS) will undervalue the risks and overvalue the potential benefits from future land uses that favour continued site activity, compared to people who live near but are not employed at SRS. We interviewed 286 people attending the Aiken Trials horse show on 14 March 1997. There were few gender differences, although men hunted and fished more than women, women ranked three environmental concerns as more severe than did men, and women were more concerned about the effect of SRS on property values. Maintenance of SRS as a National Environmental Research Park ranked first as a future land use; nuclear production ranked second, followed by hunting and hiking. Only residential development ranked very low as a future land use. There were many differences as a function of employment history at SRS: (1) people who work at SRS think that the federal government should spend funds to clean up all nuclear facilities, and they think less money should be spent on other environmental problems than did non-employees, (2) people who work at SRS ranked continued current uses of SRS higher than did people who never worked at SRS, and (3) people who work at SRS are less concerned about the storage of nuclear material or accidents at the site than are people who never worked at the site

    Evidence from phylogenetic and genome fingerprinting analyses suggests rapidly changing variation in Halorubrum and Haloarcula populations

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    Halobacteria require high NaCl concentrations for growth and are the dominant inhabitants of hypersaline environments above 15% NaCl. They are well documented to be highly recombinogenic, both in frequency and in the range of exchange partners. In this study, we examine the genetic and genomic variation of cultured, naturally co-occurring environmental populations of Halobacteria. Sequence data from multiple loci (~2500bp) identified closely related strains belonging to the genera Halorubrum and Haloarcula. Genome fingerprinting using a random priming PCR amplification method to analyze these isolates revealed diverse banding patterns within and across each of the genera and surprisingly even for isolates that are identical at the nucleotide level for five protein coding sequenced loci. This variance in genome structure even between identical multilocus sequence analysis (MLSA) haplotypes suggests that accumulation of variation is rapid, perhaps occurring every generation

    Leisingera sp. JC1, a Bacterial Isolate from Hawaiian Bobtail Squid Eggs, Produces Indigoidine and Differentially Inhibits Vibrios

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    Female members of many cephalopod species house a bacterial consortium that is part of their reproductive system, the accessory nidamental gland (ANG). These bacteria are deposited into eggs that are then laid in the environment where they must develop unprotected from predation, pathogens and fouling. In this study, we characterized the genome and secondary metabolite production of Leisingera sp. JC1, a member of the roseobacter clade (Rhodobacteraceae) of Alphaproteobacteria isolated from the jelly coat of eggs from the Hawaiian bobtail squid, Euprymna scolopes. Whole genome sequencing and MLSA analysis revealed that Leisingera sp. JC1 falls within a group of roseobacters associated with squid ANGs. Genome and biochemical analyses revealed the potential for and production of a number of secondary metabolites, including siderophores and acyl-homoserine lactones involved with quorum sensing. The complete biosynthetic gene cluster for the pigment indigoidine was detected in the genome and mass spectrometry confirmed the production of this compound. Furthermore, we investigated the production of indigoidine under co-culture conditions with Vibrio fischeri, the light organ symbiont of E. scolopes, and with other vibrios. Finally, both Leisingera sp. JC1 and secondary metabolite extracts of this strain had differential antimicrobial activity against a number of marine vibrios, suggesting that Leisingera sp. JC1 may play a role in host defense against other marine bacteria either in the eggs and/or ANG. These data also suggest that indigoidine may be partially, but not wholly, responsible for the antimicrobial activity of this squid-associated bacterium.
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