Методические указания к выполнению индивидуальных заданий практикума по дисциплине «Энергетический потенциал природных возобновляемых энергоресурсов и эффективность его преобразования в электроэнергию»

В методических указаниях рассмотрены вопросы оценки энергетического потенциала природных возобновляемых энергоресурсов: ветра, солнечного излучения и потоков воды и эффективности их преобразования в электроэнергию. Пособие предназначено для студентов дневного обучения по направлению13.04.02 «Электроэнергетика и электротехника» по профилям магистерской подготовки «Возобновляемые источники энергии» и «Оптимизация развивающихся систем электроснабжения»

Metabolic engineering of photorespiration

The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements

Transcript, protein and metabolite temporal dynamics in the CAM plant Agave

Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C3 and C4 photosynthesis) day/night pattern of stomatal closure/opening to shift CO2 uptake to the night, when evapotranspiration rates are low. A systems-level understanding of temporal molecular and metabolic controls is needed to define the cellular behaviour underpinning CAM. Here, we report high-resolution temporal behaviours of transcript, protein and metabolite abundances across a CAM diel cycle and, where applicable, compare the observations to the well-established C3 model plant Arabidopsis. A mechanistic finding that emerged is that CAM operates with a diel redox poise that is shifted relative to that in Arabidopsis. Moreover, we identify widespread rescheduled expression of genes associated with signal transduction mechanisms that regulate stomatal opening/closing. Controlled production and degradation of transcripts and proteins represents a timing mechanism by which to regulate cellular function, yet knowledge of how this molecular timekeeping regulates CAM is unknown. Here, we provide new insights into complex post-transcriptional and -translational hierarchies that govern CAM in Agave. These data sets provide a resource to inform efforts to engineer more efficient CAM traits into economically valuable C3 crops