Permutations preserving divisibility

We give a proof of a theorem on the common divisibility of polynomials and permuted polynomials (over GF(2)) by a polynomial g(x)

Identification and characterization of DAMs mutations associated with early blooming in sweet cherry, and validation of DNA-based markers for selection

Dormancy release and bloom time of sweet cherry cultivars depend on the environment and the genotype. The knowledge of these traits is essential for cultivar adaptation to different growing areas, and to ensure fruit set in the current climate change scenario. In this work, the major sweet cherry bloom time QTL qP-BT1.1m (327 Kbs; Chromosome 1) was scanned for candidate genes in the Regina cv genome. Six MADS-box genes (PavDAMs), orthologs to peach and Japanese apricot DAMs, were identified as candidate genes for bloom time regulation. The complete curated genomic structure annotation of these genes is reported. To characterize PavDAMs intra-specific variation, genome sequences of cultivars with contrasting chilling requirements and bloom times (N = 13), were then mapped to the ‘Regina’ genome. A high protein sequence conservation (98.8–100%) was observed. A higher amino acid variability and several structural mutations were identified in the low-chilling and extra-early blooming cv Cristobalina. Specifically, a large deletion (694 bp) upstream of PavDAM1, and various INDELs and SNPs in contiguous PavDAM4 and -5 UTRs were identified. PavDAM1 upstream deletion in ‘Cristobalina’ revealed the absence of several cis-acting motifs, potentially involved in PavDAMs expression. Also, due to this deletion, a non-coding gene expressed in late-blooming ‘Regina’ seems truncated in ‘Cristobalina’. Additionally, PavDAM4 and -5 UTRs mutations revealed different splicing variants between ‘Regina’ and ‘Cristobalina’ PavDAM5. The results indicate that the regulation of PavDAMs expression and post-transcriptional regulation in ‘Cristobalina’ may be altered due to structural mutations in regulatory regions. Previous transcriptomic studies show differential expression of PavDAM genes during dormancy in this cultivar. The results indicate that ‘Cristobalina’ show significant amino acid differences, and structural mutations in PavDAMs, that correlate with low-chilling and early blooming, but the direct implication of these mutations remains to be determined. To complete the work, PCR markers designed for the detection of ‘Cristobalina’ structural mutations in PavDAMs, were validated in an F2 population and a set of cultivars. These PCR markers are useful for marker-assisted selection of early blooming seedlings, and probably low-chilling, from ‘Cristobalina’, which is a unique breeding source for these traits. © Copyright © 2021 Calle, Grimplet, Le Dantec and Wünsch

An inexpensive nonlinear medium for intense ultrabroadband pulse characterization

The ability of pellets made up of compressed iron iodate nanocrystals to frequency-double the whole visible spectrum is demonstrated. We suggest their use for complete characterization of intense ultrabroadband laser pulse

Digital Design Considerations for Volunteer Recruitment: Making the Implicit Promises of Volunteering More Explicit

Non-profit organisations may find it difficult to demonstrate to potential volunteers what is required in their voluntary role-resulting in a mismatch between expectations and reality for volunteers. This mismatch could be perceived as a psychological contract breach. We interviewed 18 volunteers and 7 coordinators about their experiences and expectations in order to understand how the experience of volunteers can better be captured and communicated. Further, we wished to consider how future digital platforms might capture important elements of the volunteer experience to better support recruitment, retention and recognition. We present our findings and discuss digital platform implications around the four implicit 'promises' of volunteering: the social promise, the opportunity promise, the value promise and the organisational citizenship promise. We add to literature exploring the voluntary sector by assessing the feasibility of digital interventions to support various aspects of volunteer and coordinator roles

Sediment dynamics in the subaquatic channel of the Rhone delta (Lake Geneva, France/Switzerland)

With its smaller size, well-known boundary conditions, and the availability of detailed bathymetric data, Lake Geneva's subaquatic canyon in the Rhone Delta is an excellent analogue to understand sedimentary processes in deep-water submarine channels. A multidisciplinary research effort was undertaken to unravel the sediment dynamics in the active canyon. This approach included innovative coring using the Russian MIR submersibles, in situ geotechnical tests, and geophysical, sedimentological, geochemical and radiometric analysis techniques. The canyon floor/levee complex is characterized by a classic turbiditic system with frequent spillover events. Sedimentary evolution in the active canyon is controlled by a complex interplay between erosion and sedimentation processes. In situ profiling of sediment strength in the upper layer was tested using a dynamic penetrometer and suggests that erosion is the governing mechanism in the proximal canyon floor while sedimentation dominates in the levee structure. Sedimentation rates progressively decrease down-channel along the levee structure, with accumulation exceeding 2.6cm/year in the proximal levee. A decrease in the frequency of turbidites upwards along the canyon wall suggests a progressive confinement of the flow through time. The multi-proxy methodology has also enabled a qualitative slope-stability assessment in the levee structure. The rapid sediment loading, slope undercutting and over-steepening, and increased pore pressure due to high methane concentrations hint at a potential instability of the proximal levees. Furthermore, discrete sandy intervals show very high methane concentrations and low shear strength and thus could correspond to potentially weak layers prone to scarp failures

Tectonic controls on nearshore sediment accumulation and submarine canyon morphology offshore La Jolla, Southern California

CHIRP seismic and swath bathymetry data acquired offshore La Jolla, California provide an unprecedented three-dimensional view of the La Jolla and Scripps submarine canyons. Shore-parallel patterns of tectonic deformation appear to control nearshore sediment thickness and distribution around the canyons. These shore-parallel patterns allow the impact of local tectonic deformation to be separated from the influence of eustatic sea-level fluctuations. Based on stratal geometry and acoustic character, we identify a prominent angular unconformity inferred to be the transgressive surface and three sedimentary sequences: an acoustically laminated estuarine unit deposited during early transgression, an infilling or “healing-phase” unit formed during the transgression, and an upper transparent unit. Beneath the transgressive surface, steeply dipping reflectors with several dip reversals record faulting and folding along the La Jolla margin. Scripps Canyon is located at the crest of an antiform, where the rocks are fractured and more susceptible to erosion. La Jolla Canyon is located along the northern strand of the Rose Canyon Fault Zone, which separates Cretaceous lithified rocks to the south from poorly cemented Eocene sands and gravels to the north. Isopach and structure contour maps of the three sedimentary units reveal how their thicknesses and spatial distributions relate to regional tectonic deformation. For example, the estuarine unit is predominantly deposited along the edges of the canyons in paleotopographic lows that may have been inlets along barrier beaches during the Holocene sea-level rise. The distribution of the infilling unit is controlled by pre-existing relief that records tectonic deformation and erosional processes. The thickness and distribution of the upper transparent unit are controlled by long-wavelength, tectonically induced relief on the transgressive surface and hydrodynamics

A linguistic approach to assess the dynamics of design team preference in concept selection

This paper addresses the problem of describing the decision-making process of a committee of engineers based upon their verbalized linguistic appraisals of alternatives. First, we show a way to model an individual’s evaluation of an alternative through natural language based on the Systemic-Functional Linguistics system of APPRAISAL. The linguistic model accounts for both the degree of intensity and the uncertainty of expressed evaluations. Second, this multi-dimensional linguistic model is converted into a scalar to represent the degree of intensity and a probability distribution function for the stated evaluation. Finally, we present a Markovian model to calculate the time-varying change in preferential probability, the probability that an alternative is the most preferred alternative. We further demonstrate how preferential probability toward attributes of alternatives correspond to preferential probability toward alternatives. We illustrate the method on two case studies to highlight the time-variant dynamics of preferences toward alternatives and attributes. This research contributes to process tracing in descriptive decision science to understand how engineers actually take decisions.National Science Foundation (U.S.) (Award CMMI-0900255

Prospecting environmental mycobacteria: combined molecular approaches reveal unprecedented diversity

Background: Environmental mycobacteria (EM) include species commonly found in various terrestrial and aquatic environments, encompassing animal and human pathogens in addition to saprophytes. Approximately 150 EM species can be separated into fast and slow growers based on sequence and copy number differences of their 16S rRNA genes. Cultivation methods are not appropriate for diversity studies; few studies have investigated EM diversity in soil despite their importance as potential reservoirs of pathogens and their hypothesized role in masking or blocking M. bovis BCG vaccine. Methods: We report here the development, optimization and validation of molecular assays targeting the 16S rRNA gene to assess diversity and prevalence of fast and slow growing EM in representative soils from semi tropical and temperate areas. New primer sets were designed also to target uniquely slow growing mycobacteria and used with PCR-DGGE, tag-encoded Titanium amplicon pyrosequencing and quantitative PCR. Results: PCR-DGGE and pyrosequencing provided a consensus of EM diversity; for example, a high abundance of pyrosequencing reads and DGGE bands corresponded to M. moriokaense, M. colombiense and M. riyadhense. As expected pyrosequencing provided more comprehensive information; additional prevalent species included M. chlorophenolicum, M. neglectum, M. gordonae, M. aemonae. Prevalence of the total Mycobacterium genus in the soil samples ranged from 2.3×107 to 2.7×108 gene targets g−1; slow growers prevalence from 2.9×105 to 1.2×107 cells g−1. Conclusions: This combined molecular approach enabled an unprecedented qualitative and quantitative assessment of EM across soil samples. Good concordance was found between methods and the bioinformatics analysis was validated by random resampling. Sequences from most pathogenic groups associated with slow growth were identified in extenso in all soils tested with a specific assay, allowing to unmask them from the Mycobacterium whole genus, in which, as minority members, they would have remained undetected

Phthiocerol Dimycocerosates of M. tuberculosis Participate in Macrophage Invasion by Inducing Changes in the Organization of Plasma Membrane Lipids

Phthiocerol dimycocerosates (DIM) are major virulence factors of Mycobacterium tuberculosis (Mtb), in particular during the early step of infection when bacilli encounter their host macrophages. However, their cellular and molecular mechanisms of action remain unknown. Using Mtb mutants deleted for genes involved in DIM biosynthesis, we demonstrated that DIM participate both in the receptor-dependent phagocytosis of Mtb and the prevention of phagosomal acidification. The effects of DIM required a state of the membrane fluidity as demonstrated by experiments conducted with cholesterol-depleting drugs that abolished the differences in phagocytosis efficiency and phagosome acidification observed between wild-type and mutant strains. The insertion of a new cholesterol-pyrene probe in living cells demonstrated that the polarity of the membrane hydrophobic core changed upon contact with Mtb whereas the lateral diffusion of cholesterol was unaffected. This effect was dependent on DIM and was consistent with the effect observed following DIM insertion in model membrane. Therefore, we propose that DIM control the invasion of macrophages by Mtb by targeting lipid organisation in the host membrane, thereby modifying its biophysical properties. The DIM-induced changes in lipid ordering favour the efficiency of receptor-mediated phagocytosis of Mtb and contribute to the control of phagosomal pH driving bacilli in a protective niche