Temperature and Crowding Effects on Virus Manifestation in Neodiprion Sertifer (Hymenoptera: Diprionidae) Larvae

Temperature and (or) crowding (larval density) functioned as stressors in the induction of symptoms associated with the nucleopolyhedrosis virus of the European pine sawfly, Neodiprion sertifer, Subsamptes of larvae maintained at 30 and 35°C, with three levels of larval density each (20, 60, and 100/shoot) which had died under these conditions, revealed the presence of polyhedral inclusion bodies under microscopic examination. In contrast, larvae maintained at 25°C with the same three larval density levels experienced no symptoms of virus infection or mortality, The latter was consistent with field observations when temperatures during larval development ranged from 14°C to 27°C and larval densities were in the same general range

Bioassay of the Nucleopolyhedrosis Virus of \u3ci\u3eNeodiprion Sertifer\u3c/i\u3e (Hymenoptera: Diprionidae)

Linear regression analysis of probit mortality versus several concentrations of nucleo- polyhedrosis virus of Neodiprion sertifer resulted in the equation Y = 2.170 + 0.872X. An LC50 was calculated at 1758 PIB/mL Also, the incubation time of the virus was dependent on Its concentration

Field Release of Virus-Sprayed Adult Parasitoids of the European Pine Sawfly (Hymenoptera: Diprionidae) in Wisconsin

Rapid field release of adult parasitoids sprayed with the nucleopolyhedrosis virus of the European pine sawfly successfully transferred the virus to feeding larval colonies

A comparison of the quality of image acquisition between two different sidestream dark field video-microscopes

Sidestream dark field (SDF) imaging enables direct visualisation of the microvasculature from which quantification of key variables is possible. The new MicroScan USB3 (MS-U) video-microscope is a hand-held SDF device that has undergone significant technical upgrades from its predecessor, the MicroScan Analogue (MS-A). The MS-U claims superior quality of sublingual microcirculatory image acquisition over the MS-A, however, this has yet to be robustly confirmed. In this manuscript, we therefore compare the quality of image acquisition between these two devices. The microcirculation of healthy volunteers was visualised to generate thirty video images for each device. Two independent raters, blinded to the device type, graded the quality of the images according to the six different traits in the Microcirculation Image Quality Score (MIQS) system. Chi-squared tests and Kappa statistics were used to compare not only the distribution of scores between the devices, but also agreement between raters. MS-U showed superior image quality over MS-A in three of out six MIQS traits; MS-U had significantly more optimal images by illumination (MS-U 95% optimal images, MS-A 70% optimal images (p-value 0.003)), by focus (MS-U 70% optimal images, MS-A 35% optimal images (p-value 0.002)) and by pressure (MS-U 72.5% optimal images, MS-A 47.5% optimal images (p-value 0.02)). For each trait, there was at least 85% agreement between the raters, and all the scores for each trait were independent of the rater (all p-values > 0.05). These results show that the new MS-U provides a superior quality of sublingual microcirculatory image acquisition when compared to old MS-

Changes in labial capillary density on ascent to and descent from high altitude.

Present knowledge of how the microcirculation is altered by prolonged exposure to hypoxia at high altitude is incomplete and modification of existing analytical techniques may improve our knowledge considerably. We set out to use a novel simplified method of measuring in vivo capillary density during an expedition to high altitude using a CytoCam incident dark field imaging video-microscope. The simplified method of data capture involved recording one-second images of the mucosal surface of the inner lip to reveal data about microvasculature density in ten individuals. This was done on ascent to, and descent from, high altitude. Analysis was conducted offline by two independent investigators blinded to the participant identity, testing conditions and the imaging site.  Additionally we monitored haemoglobin concentration and haematocrit data to see if we could support or refute mechanisms of altered density relating to vessel recruitment. Repeated sets of paired values were compared using Kruskall Wallis Analysis of Variance tests, whilst comparisons of values between sites was by related samples Wilcoxon Signed Rank Test. Correlation between different variables was performed using Spearman's rank correlation coefficient, and concordance between analysing investigators using intra-class correlation coefficient. There was a significant increase in capillary density from London on ascent to high altitude; median capillaries per field of view area increased from 22.8 to 25.3 (p=0.021). There was a further increase in vessel density during the six weeks spent at altitude (25.3 to 32.5, p=0.017). Moreover, vessel density remained high on descent to Kathmandu (31.0 capillaries per field of view area), despite a significant decrease in haemoglobin concentration and haematocrit. Using a simplified technique, we have demonstrated an increase in capillary density on early and sustained exposure to hypobaric hypoxia at thigh altitude, and that this remains elevated on descent to normoxia. The technique is simple, reliable and reproducible

Isomerization dynamics of a buckled nanobeam

We analyze the dynamics of a model of a nanobeam under compression. The model is a two mode truncation of the Euler-Bernoulli beam equation subject to compressive stress. We consider parameter regimes where the first mode is unstable and the second mode can be either stable or unstable, and the remaining modes (neglected) are always stable. Material parameters used correspond to silicon. The two mode model Hamiltonian is the sum of a (diagonal) kinetic energy term and a potential energy term. The form of the potential energy function suggests an analogy with isomerisation reactions in chemistry. We therefore study the dynamics of the buckled beam using the conceptual framework established for the theory of isomerisation reactions. When the second mode is stable the potential energy surface has an index one saddle and when the second mode is unstable the potential energy surface has an index two saddle and two index one saddles. Symmetry of the system allows us to construct a phase space dividing surface between the two "isomers" (buckled states). The energy range is sufficiently wide that we can treat the effects of the index one and index two saddles in a unified fashion. We have computed reactive fluxes, mean gap times and reactant phase space volumes for three stress values at several different energies. In all cases the phase space volume swept out by isomerizing trajectories is considerably less than the reactant density of states, proving that the dynamics is highly nonergodic. The associated gap time distributions consist of one or more `pulses' of trajectories. Computation of the reactive flux correlation function shows no sign of a plateau region; rather, the flux exhibits oscillatory decay, indicating that, for the 2-mode model in the physical regime considered, a rate constant for isomerization does not exist.Comment: 42 pages, 6 figure

Nonlinear modes for the Gross-Pitaevskii equation -- demonstrative computation approach

A method for the study of steady-state nonlinear modes for Gross-Pitaevskii equation (GPE) is described. It is based on exact statement about coding of the steady-state solutions of GPE which vanish as $x\to+\infty$ by reals. This allows to fulfill {\it demonstrative computation} of nonlinear modes of GPE i.e. the computation which allows to guarantee that {\it all} nonlinear modes within a given range of parameters have been found. The method has been applied to GPE with quadratic and double-well potential, for both, repulsive and attractive nonlinearities. The bifurcation diagrams of nonlinear modes in these cases are represented. The stability of these modes has been discussed.Comment: 21 pages, 6 figure

Libros de cine

Pena, JJ.; Cerdán, J.; Nogueira, X.; Parrondo Coppel, E. (1995). Libros de cine. Vértigo. Revista de cine. (12):76-79. http://hdl.handle.net/10251/43047.Importación Masiva76791

Initial evidence for the criterion-related and structural validity of the long versions of the direct and meta-perspectives of the Coach-Athlete Relationship Questionnaire

This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2010 Taylor & Francis.The aim of the present study was to develop and initially validate a longer version of the direct (Jowett & Ntoumanis, 2004) and meta-perspectives (Jowett, 2009a, 2009b) of the Coach-Athlete Relationship Questionnaire (CART-Q). In Study 1, instruments (e.g. questionnaires, scales, and inventories) that have been used to assess relationship quality in the broader psychological literature were examined and items potentially relevant to the coach-athlete relationship were identified. The content validity of the identified items was then assessed using expert panels. A final questionnaire was subsequently prepared and administered to 693 participants (310 coaches and 383 athletes). Confirmatory factor analysis was employed to assess the multidimensional nature of the questionnaire based on the 3Cs (i.e. closeness, commitment, and complementarity) model of the coach-athlete relationship. The findings indicated that the direct and meta-perspective items of the long versions of the CART-Q approached an adequate data fit. Moreover, evidence for the internal consistency and criterion validity of the new instruments was also obtained. In Study 2, the newly developed measure was administered to an independent sample of 251 individuals (145 athletes and 106 coaches). Further statistical support was gained for the factorial validity and reliability of the longer version of the CART-Q