Interpretation of Solar Magnetic Field Strength Observations

This study based on longitudinal Zeeman effect magnetograms and spectral line scans investigates the dependence of solar surface magnetic fields on the spectral line used and the way the line is sampled in order to estimate the magnetic flux emerging above the solar atmosphere and penetrating to the corona from magnetograms of the Mt. Wilson 150-foot tower synoptic program (MWO). We have compared the synoptic program \lambda5250\AA line of Fe I to the line of Fe I at \lambda5233\AA since this latter line has a broad shape with a profile that is nearly linear over a large portion of its wings. The present study uses five pairs of sampling points on the $\lambda5233$\AA line. We recommend adoption of the field determined with a line bisector method with a sampling point as close as possible to the line core as the best estimate of the emergent photospheric flux. The combination of the line profile measurements and the cross-correlation of fields measured simultaneously with \lambda5250\AA and \lambda5233\AA yields a formula for the scale factor 1/\delta that multiplies the MWO synoptic magnetic fields. The new calibration shows that magnetic fields measured by the MDI system on the SOHO spacecraft are equal to 0.619+/-0.018 times the true value at a center-to-limb position 30 deg. Berger and Lites (2003) found this factor to be 0.64+/-0.013 based on a comparison the the Advanced Stokes Polarimeter.Comment: Accepted by Solar Physic

Automated whole-cell patch-clamp electrophysiology of neurons in vivo

Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.National Institutes of Health (U.S.) (NIH EUREKA Award program (1R01NS075421))National Institutes of Health (U.S.) ((NIH) Director′s New Innovator Award (DP2OD002002)National Science Foundation (U.S.) ((NSF) CAREER award (CBET 1053233))New York Stem Cell Foundation (Robertson Neuroscience Award)Dr. Gerald Burnett and Marjorie BurnettNational Science Foundation (U.S.) (grant CISE 1110947)National Science Foundation (U.S.) (grant EHR 0965945)American Heart Association (10GRNT4430029

SAP97 and Cortactin Remodeling in Arrhythmogenic Purkinje Cells

Because structural remodeling of several proteins, including ion channels, may underlie the abnormal action potentials of Purkinje cells (PCs) that survive in the 48 hr infarcted zone of the canine heart (IZPCs), we sought to determine the subcellular structure and function of the KV1.5 (KCNA5) protein in single IZPCs. Clustering of the Kv1.5 subunit in axons is regulated by a synapse-associated protein, SAP97, and is linked to an actin-binding protein, cortactin, and an intercellular adhesion molecule, N-cadherin. To understand the functional remodeling of the Kv1.5 channel and its regulation in IZPCs, Kv1.5 currents in PCs were measured as the currents blocked by 10 µM RSD1379 using patch-clamp techniques. Immunocytochemistry and confocal imaging were used for both single and aggregated IZPCs vs normal PCs (NZPCs) to determine the relationship of Kv1.5 with SAP-97, cortactin and N-cadherin. In IZPCs, both the sarcolemma (SL) and intercalated disk (ID) Kv1.5 protein are abundant, and the amount of cytosolic Kv1.5 protein is greatly increased. SAP-97 is also increased at IDs and has notable cytosolic localization suggesting that SAP-97 may regulate the functional expression and stabilization of Kv1.5 channels in IZPCs. Cortactin, which is located with N-cadherin at IDs in NZPCs, remains at IDs but begins to dissociate from N-cadherin, often forming ring structures and colocalizing with Kv1.5 within IZPCs. At the same time, cortactin/Kv1.5 colocalization is increased at the ID, suggesting an ongoing active process of membrane trafficking of the channel protein. Finally, the Kv1.5 current, measured as the RSD1379-sensitive current, at +40 mV did not differ between NZPCs (0.81±0.24 pA/pF, n = 14) and IZPCs (0.83±0.21 pA/pF, n = 13, NS). In conclusion, the subcellular structural remodeling of Kv1.5, SAP97 and cortactin maintained and normalized the function of the Kv1.5 channel in Purkinje cells that survived myocardial infarction

The discursive construction of childhood and youth in AIDS interventions in Lesotho's education sector: Beyond global-local dichotomies

This is the post-print version of this article. The definitive, peer-reviewed and edited version of this article is published in Environment and Planning D,Society and Space 28(5) 791 – 810, 2010, available from the link below. Copyright @ 2010 Pion.In southern Africa interventions to halt the spread of AIDS and address its social impacts are commonly targeted at young people, in many cases through the education sector. In Lesotho, education-sector responses to AIDS are the product of negotiation between a range of ‘local’ and ‘global’ actors. Although many interventions are put forward as government policy and implemented by teachers in schools, funding is often provided by bilateral and multilateral donors, and the international ‘AIDS industry’—in the form of UN agencies and international NGOs—sets agendas and makes prescriptions. This paper analyses interviews conducted with policy makers and practitioners in Lesotho and a variety of documents, critically examining the discourses of childhood and youth that are mobilised in producing changes in education policy and practice to address AIDS. Focusing on bursary schemes, life-skills education, and rights-based approaches, the paper concludes that, although dominant ‘global’ discourses are readily identified, they are not simply imported wholesale from the West, but rather are transformed through the organisations and personnel involved in designing and implementing interventions. Nonetheless, the connections through which these discourses are made, and children are subjectified, are central to the power dynamics of neoliberal globalisation. Although the representations of childhood and youth produced through the interventions are hybrid products of local and global discourses, the power relations underlying them are such that they, often unintentionally, serve a neoliberal agenda by depicting young people as individuals in need of saving, of developing personal autonomy, or of exercising individual rights.RGS-IB

Balancing school and work with new opportunities:changes in children’s gendered time use in Ethiopia (2006–2013)

We explore the temporal dimension of childhood, through time use of boys and girls in Ethiopia, focusing on the relationship between children's work and school attendance. We argue that children's time use reflects both current exigencies and more strategic future-orientated considerations, with work mainly serving the former, and education, the latter. We compare two cohorts of children aged 12 years from Young Lives longitudinal study, interviewed at two different points in time, 2006 and 2013. We examine the role of education aspirations, labour demand and structural factors such as household wealth and composition. Contrary to expectations, increased returns to work in rural areas have lowered boys' education aspirations and increased their school drop-out rates relative to girls'. Though time allocation is correlated with educational aspirations, we demonstrate that aspirations are not static, and change over childhood; locality and everyday exigencies interact with gender in reshaping children's aspirations and time-use

Search for Short-Term Periodicities in the Sun's Surface Rotation: A Revisit

The power spectral analyses of the Sun's surface equatorial rotation rate determined from the Mt. Wilson daily Doppler velocity measurements during the period 3 December 1985 to 5 March 2007 suggests the existence of 7.6 year, 2.8 year, 1.47 year, 245 day, 182 day and 158 day periodicities in the surface equatorial rotation rate during the period before 1996. However, there is no variation of any kind in the more accurately measured data during the period after 1995. That is, the aforementioned periodicities in the data during the period before the year 1996 may be artifacts of the uncertainties of those data due to the frequent changes in the instrumentation of the Mt. Wilson spectrograph. On the other hand, the temporal behavior of most of the activity phenomena during cycles 22 (1986-1996) and 23 (after 1997) is considerably different. Therefore, the presence of the aforementioned short-term periodicities during the last cycle and absence of them in the current cycle may, in principle, be real temporal behavior of the solar rotation during these cycles.Comment: 11 pages, 6 figures, accepted for publication in Solar Physic

Recording temporal data with minutes resolution into DNA

Recording complex biological signals is a crucial application of synthetic biology and essential for a deeper understanding of biological processes. An ideal “biorecorder” would have the ability to record biological signals over a wide spatial distribution of cells with high temporal resolution. However, the genetically encoded biorecording tools available have very good spatial resolution (cellular level), but currently rely on turning on and off transcription and translation of a protein (e.g., Cas9 or a recombinase) to record the biological signal, making their temporal resolution on the order of hours. Here we introduce a DNA polymerase based biorecorder that can record cationic concentration fluctuations into DNA sequence with a resolution of ~1 minute. We use a template independent DNA polymerase; terminal deoxynucleotidyl transferase (TdT) that randomly incorporates bases onto a single strand of DNA. The preference of base incorporated by TdT changes with the concentration of cations in TdT’s environment. Therefore, by analyzing a strand of DNA that was extended in fluctuating cation concentrations, we can determine the temporal profile of cation concentration from the bases added. Using this method, we can measure a change in Co2+ concentration during a one hour period with an accuracy of 1 min. We also show the approach works for Zn2+ and Ca2+. We will present our methods for optimizing this biorecorder and characterize its performance in vitro. Recording data onto DNA with minutes time resolution could solve many challenging data acquisition problems in neuroscience and developmental biology, and could aid in the use of DNA as a data storage medium

New datings and elevations of a fossil reef in Lembetabe, southwest Madagascar: eustatic and tectonic implications

The study of geological sea-level proxies formed during previous interglacials is a common approach to assess how global sea level will evolve under warmer climate conditions. Over the last decades, technical advancements in both survey and geochronology have allowed improving our knowledge of past sea-level highstands. This is of prime importance to refine our understanding of processes contributing to sea-level changes, and ultimately to improve both local and global sea-level projections. Last Interglacial sea-level proxies in the Western Indian Ocean (and more specifically the island nation of Madagascar), have been less investigated than in other intertropical oceans over the last decades. As a result, paleo sea-level data in this region are less abundant and less precise than elsewhere. Here, we report the results of two field campaigns aimed at studying the site of Lembetabe, southwest Madagascar, where a fossil reef was first described by the researcher Rene ⠁ Battistini more than 50 years ago. We estimate paleo relative sea level history in space and time from 15 new U-series ages from a fossil reef platform mapped with differential GNSS and drone photogrammetry. Our data suggest that, between 129 ka and 115 ka, paleo relative sea level at this location was about 3.4 & PLUSMN; 1.4 m above modern. Once corrected for glacial isostatic adjustment, we find that paleo global mean sea level did not exceed 3 m above modern. Only slight crustal subsidence would reconcile the peak Last Interglacial sea level measured at Lembetabe with the 5 e10 m range reported in the literature.& COPY; 2023 Published by Elsevier Ltd

Early IFNγ-Mediated and Late Perforin-Mediated Suppression of Pathogenic CD4 T Cell Responses Are Both Required for Inhibition of Demyelinating Disease by CNS-Specific Autoregulatory CD8 T Cells

Pathogenesis of immune-mediated demyelinating diseases like multiple sclerosis (MS) is thought to be governed by a complex cellular interplay between immunopathogenic and immunoregulatory responses. We have previously shown that central nervous system (CNS)-specific CD8 T cells have an unexpected protective role in the mouse model of MS, experimental autoimmune encephalomyelitis (EAE). In this study, we interrogated the suppressive potential of PLP178-191-specific CD8 T cells (PLP-CD8). Here, we show that PLP-CD8, when administered post-disease onset, rapidly ameliorated EAE progression, and suppressed PLP178-191-specific CD4 T cell responses as measured by delayed-type hypersensitivity (DTH). To accomplish DTH suppression, PLP-CD8 required differential production of perforin and IFNγ. Perforin was not required for the rapid suppressive action of these cells, but was critical for maintenance of optimal longer term DTH suppression. Conversely, IFNγ production by PLP-CD8 was necessary for swift DTH suppression, but was less significant for maintenance of longer term suppression. These data indicate that CNS-specific CD8 T cells employ an ordered regulatory mechanism program over a number of days in vivo during demyelinating disease and have mechanistic implications for this immunotherapeutic approach

Neuroma of a double gallbladder: a case report

We report a case of 55 year old male patient who presented with recurrent upper abdominal pain following a laparoscopic cholecystectomy. A subsequent diagnostic laparoscopy revealed the presence of a second gallbladder which was initially missed. The peculiarity of his symptoms can in part be explained by the presence of a traumatic neuroma in his second gallbladder. A subsequent cholecystectomy led to a complete resolution of this patient's signs and symptoms. As far as we know this is the first report in the literature of a traumatic neuroma in a second gallbladder