Tafel-analysis of the AP-CITROX decontamination technology of Inconel alloy 690

International audienceSafe, effective, cost-effective, easy feasible and low-waste decontamination technologies are fundamental importance from environmental and radiation protection aspects. In this study the effectiveness of AP-CITROX decontamination technology of Inconel alloy 690 was investigated. Non-radioactive representative metal samples were formed to test of decontamination technology and the clear-, the corroded-, the decontaminated layer were analysed electrochemically. The results indicate that the passivation step of the technology was not completed

Gene expression profiling in PC12 cells infected with an oncolytic Newcastle disease virus strain

Although the oncolytic potential of natural, non-engineered Newcastle disease virus (NDV) isolates are well-known, cellular mechanisms determining NDV sensitivity of tumor cells are poorly understood. The aim of the present study was to look for gene expression changes in PC12 pheochromocytoma cells infected with an attenuated NDV strain that may be related to NDV susceptibility. PC12 cells were infected with the NDV strain MTH-68/H for 12hours at a titer corresponding to the IC50 value. Total cytoplasmic RNA samples isolated from control and MTH-68/H-infected cells were analyzed using a rat specific Affymetrix exon chip. Genes with at least 2-fold increase or decrease in their expression were identified. MTH-68/H-induced gene expression changes of 9 genes were validated using quantitative reverse transcriptase PCR. A total of 729 genes were up- and 612 genes were down-regulated in PC12 cells infected with MTH-68/H. Using the DAVID functional annotation clustering tool, the up- and down-regulated genes can be categorized into 176 and 146 overlapping functional gene clusters, respectively. Gene expression changes affecting the most important signaling mechanisms (Toll-like receptor signaling, RIG-I-like receptor signaling, interferon signaling, interferon effector pathways, apoptosis pathways, endoplasmic reticulum stress pathways, cell cycle regulation) are analyzed and discussed in detail in this paper. NDV-induced gene expression changes described in this paper affect several regulatory mechanisms and dozens of putative key proteins that may determine the NDV susceptibility of various tumors. Further characterization of these proteins may identify susceptibility markers to predict the chances of virotherapeutic treatment of human tumors

State Of the Art Report in the fields of numerical analysis and scientific computing

This document provides an assessment of the current state-of-the-art for the DONUT work package. It is intended as a reference for the involved actors in EURAD and will be updated at the end of the project as new information becomes available. The prupose of this work package is to improve/develop methods or numerical tools in order to go a step further in development of (i) relevant, performant and cutting-edge numerical methods that can easily be implemented in existing or new tools, in order to carry out high-performance computing to facilitate the study of highly coupled processes in large systems. These methods and their implementation in tools will be mainly applied to reactive transport, 2-phase flow, and THM modelling in porous and fractured media; (ii) numerical scale transition schemes for coupled processes (meso1 to macro scale), supporting the study of specific multi-scale couplings such as chemo-mechanics; (iii) innovative numerical methods to carry out uncertainty and sensitivity analyses coupled processes.The readers have to keep in mind that this report is not reviewing all the existing codes, methods or tools that are available in the literature. It is rather written and oriented in the perspective of the research program that will be conducted by the different partners within DONUT. While some general statement are given, the research direction that will be followed by partners are outlined