160 research outputs found

    Asynchronous Multi-Context Systems

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    In this work, we present asynchronous multi-context systems (aMCSs), which provide a framework for loosely coupling different knowledge representation formalisms that allows for online reasoning in a dynamic environment. Systems of this kind may interact with the outside world via input and output streams and may therefore react to a continuous flow of external information. In contrast to recent proposals, contexts in an aMCS communicate with each other in an asynchronous way which fits the needs of many application domains and is beneficial for scalability. The federal semantics of aMCSs renders our framework an integration approach rather than a knowledge representation formalism itself. We illustrate the introduced concepts by means of an example scenario dealing with rescue services. In addition, we compare aMCSs to reactive multi-context systems and describe how to simulate the latter with our novel approach.Comment: International Workshop on Reactive Concepts in Knowledge Representation (ReactKnow 2014), co-located with the 21st European Conference on Artificial Intelligence (ECAI 2014). Proceedings of the International Workshop on Reactive Concepts in Knowledge Representation (ReactKnow 2014), pages 31-37, technical report, ISSN 1430-3701, Leipzig University, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-15056

    Towards a lightweight mobile semantic-based approach for enhancing interaction with smart objects

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    This work describes a semantic extension for a user-smart object interaction model based on the ECA paradigm (Event-Condition-Action). In this approach, smart objects publish their sensing (event) and action capabilities in the cloud and mobile devices are prepared to retrieve them and act as mediators to configure personalized behaviours for the objects. In this paper, the information handled by this interaction system has been shaped according several semantic models that, together with the integration of an embedded ontological and rule-based reasoner, are exploited in order to (i) automatically detect incompatible ECA rules configurations and to (ii) support complex ECA rules definitions and execution. This semantic extension may significantly improve the management of smart spaces populated with numerous smart objects from mobile personal devices, as it facilitates the configuration of coherent ECA rules

    A tuberculosis molecular bacterial load assay (TB-MBLA)

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    Funding: European and Developing Countries Clinical Trials Partnership (EDCTP) – Pan African Biomarker Expansion program (PanBIOME) grant SP.2011.41304.008. Support was also obtained the University of St Andrews School of Medicine research grant.Tuberculosis is caused by Mycobacterium tuberculosis (Mtb), a pathogen classified by the United Nations (UN) as a dangerous category B biological substance. For the sake of the workers’ safety, handling of all samples presumed to carry Mtb must be conducted in a containment level (CL) 3 laboratory. The TB molecular bacterial load assay (TB-MBLA) test is a reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) test that quantifies Mtb bacillary load using primers and dual-labelled probes for 16S rRNA. We describe the use of heat inactivation to render TB samples noninfectious while preserving RNA for the TB-MBLA. A 1 mL aliquot of the sputum sample in tightly closed 15 mL centrifuge tubes is boiled for 20 min at either 80 °C, 85 °C, or 95 °C to inactivate Mtb bacilli. Cultivation of the heat inactivated and control (live) samples for 42 days confirmed the death of TB. The inactivated sample is then spiked with 100 µL of the extraction control and RNA is extracted following the standard RNA isolation procedure. No growth was observed in the cultures of heat treated samples. The isolated RNA is subjected to real-time RT-qPCR, which amplifies a specific target in the Mtb 16S rRNA gene, yielding results in the form of quantification cycles (Cq). A standard curve is used to translate Cq into bacterial load, or estimated colony forming units per mL (eCFU/mL). There is an inverse relationship between Cq and the bacterial load of a sample. The limitation is that heat inactivation lyses some cells, exposing the RNA to RNases that cause a loss of <1 log10eCFU/mL (i.e., <10 CFU/mL). Further studies will determine the proportion of very low burden patients that cause false negative results due to heat inactivation.Publisher PDFPeer reviewe

    From ''Chastity as a gift'' to ''Doing it as a sign of love'': a longitudinal analysis of the discourses on female sexuality in popular magazines in Portugal

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    Female sexuality published in six types of magazines between 1965 and 2006 were content analyzed. The results showed that Portuguese media’s discourse on sexuality is not only becoming more liberal but is also characterized by ambiguity and contradiction. Within a “progressive” discursive framework for sexuality, tensions and double standards for women are still noticeable. The media discourse in Portugal continues to reinforce the established gender hierarchy and inequality in heterosexual relationships and maintain traditional gender roles
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