The COPAS Profiler can discriminate between worms carrying different transgenic arrays and can be used to sort worms of a desired genotype

<p><b>Copyright information:</b></p><p>Taken from "A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode "</p><p></p><p>Nucleic Acids Research 2006;35(2):e11-e11.</p><p>Published online 12 Dec 2006</p><p>PMCID:PMC1903375.</p><p>© 2006 The Author(s).</p> Fluorescent micrographs (upper panels) and profiles (lower panels) for individual worms carrying the substrate array (), the transposase array (), associated with specific GFP expression in the pharynx and in the coelomocytes, respectively, or both (). Fluorescence is measured in arbitrary but constant units. Time-of-flight is a measure of length ()

Successive steps in image analysis to determine whether a well contains worms

<p><b>Copyright information:</b></p><p>Taken from "A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode "</p><p></p><p>Nucleic Acids Research 2006;35(2):e11-e11.</p><p>Published online 12 Dec 2006</p><p>PMCID:PMC1903375.</p><p>© 2006 The Author(s).</p> An image of each entire well of a 96-well plate was acquired automatically () and processed in successive steps using the ImageJ (NIH) software: overall light and contrast enhancement (), loading of a circular region and inversion of gray level at each pixel inside the region (), binarization on the basis of gray level at each pixel in the entire image (), removal of all the selected particles outside the loaded region () and removal of all the particles shorter than a minimum size ()

The metaphysical thought of Thomas Aquinas. From the concept of being and unity to the concept of whole and part

The subject-matter of the thesis is the metaphysical thought of the renowned medieval philosopher and theologian Thomas Aquinas (1224/5-1274). The main goal ofthe thesis is to expose Aquinas' part-whole doctrine (mereology) in the broad context of his metaphysical theory. In concrete, we mean to elucidate the part-whole relationship in the background ofthe key metaphysical principles and concepts, such as the notion of being and unity or the issue of the ontological structure of a categorial being. The whole thesis can be divided into two main parts. In the first part we tackle those issues, which must be outlined to comprehend Aquinas' mereology in the broad horizon of his metaphysics. In the second part we set forth the independent attempt to advance Aquinas' part-whole theory

Additional file 8: Table S7. of A quantitative genome-wide RNAi screen in C. elegans for antifungal innate immunity genes

Species used in phylogenetic clustering listed in the same order as in Fig. 8. (XLSX 47 kb

Finding <i>Mos1</i> alleles with MosLocator.

<p>(A) MosLocator (<a href="http://www.ciml.univ-mrs.fr/applications/MosLocator" target="_blank">www.ciml.univ-mrs.fr/applications/MosLocator</a>) finds <i>Mos1</i> alleles using gene sequence or transcript names. For large lists of genetic gene names, the gene sequence or transcript names can be obtained using WormMart, or here, using WormBase Converter (<a href="http://www.ciml.univ-mrs.fr/applications/WB_converter" target="_blank">www.ciml.univ-mrs.fr/applications/WB_converter</a>) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030482#pone.0030482-Engelmann1" target="_blank">[15]</a>. In the example shown, the 23 <i>ptr</i> genes were used as input. (B) Screen grabs were captured to illustrate the use of MosLocator. Left panel: a list of sequence names was entered, and the search parameters were defined. Upper right panel: a display of the output for this search. Clicking on a non-zero number displayed in either of the last two columns, for example the “2” associated with the gene T21H3.2 (<i>ptr-16</i>), generates the display shown in the inset. This is a list of the 2 <i>Mos1</i> mutant alleles that are found within the gene T21H3.2. Each allele name is hyperlinked to Wormbase. (C) A partial view of the Variation report for the <i>Mos1</i> allele <i>ttTi21065</i> found on chromosome V at Wormbase (version WS225). (D) The genomic environment of the <i>ttTi21065</i> allele is displayed. The figure is a screen-grab from Wormbase.</p

Distribution of <i>Mos1</i> alleles.

<p>(A) Graph showing the relationship between chromosome length (as a percentage of the whole nuclear genome) and the proportion of <i>Mos1</i> alleles per chromosome reported in a previous study <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030482#pone.0030482-Granger1" target="_blank">[5]</a>, and the 10,858 alleles obtained in the current project (black and red circles, respectively). The outliers, concerning chromosomes I and V, from the previous study are highlighted with lines. (B) Distribution of distances from one <i>Mos1</i> allele to the next, in a 5′ to 3′ direction along each chromosome. The graph shows the cumulative percentage of alleles that are separated by less than the indicated distance. (C) Concentration of <i>Mos1</i> alleles at the extreme right end of chromosome I (length 15,072,423 bp). The separation of the allele numbers indicates that almost all the alleles were generated independently, except in two cases (ttTi2276 and ttTi2284; ttTi13453 and ttTi13460), highlighted by an asterisk. This region was also preferentially targeted during the previous study as reflected by the presence of several cxTi alleles.</p

Genome-wide distribution of <i>Mos1</i> mutant alleles.

<p>Chr: chromosome.</p

Summary of <i>Mos1</i> mutant production and characterization.

<p>Summary of <i>Mos1</i> mutant production and characterization.</p

Genomic coverage of <i>Mos1</i>.

<p>Graphical representation of each <i>C. elegans</i> chromosome showing the regions of the genome that are potentially amenable to genome engineering using the publicly-available <i>Mos1</i> alleles; it is assumed that any point up to 1.5 kb away from a transposon-insertion site can be targeted. The bottom line is a magnified view of the boxed region on chromosome X.</p