Morphological and antioxidant characteristics of quince (Cydonia oblonga Mill.) and chinese quince fruit (Pseudocydonia sinensis Schneid.)

Quince (Cydonia oblonga Mill.) is a fruit species, whose fruits have a high therapeutic value and therefore are used in many countries in traditional medicine. Chinese quince (Pseudocydonia sinensis Schneid.) is a lesser used species, although it is a relative of quince. The aim of the study was to evaluate some morphological characters of both kinds of fruit and antioxidant activity of morphological parts of the fruit. For these experiments, two genotypes were used from each species growing in the Arboretum Mlyňany (Slovakia). We determined the antioxidant activity of different parts by the DPPH method. In the genotypes from the evaluated species C. oblonga/P. sinensis we determined the average weight of the fruit in the fresh condition to be in the range 147.61 - 253.27 g / 197.85 - 466.38 g, the exocarp weight 28.50 - 43.89 g / 24.85 - 45.10 g, the mesocarp weight 116.36 - 204.99 g / 160.30 - 389.80 g, the seed weight 1.05 - 1.54 g / 9.22 - 17.42 g, the height of fruit 74.09 - 80.88 mm / 98.06 - 124.48 mm, average of fruit 60.11 - 81.51 mm / 62.33 - 88.64 mm. In aqueous extracts we determined antioxidant activity of the species C. oblonga / P. sinensis in dry exocarp in the range 43.52 - 67.73% / 52.76 - 82.20%, in fresh mesocarp 7.36 -14.78% / 15.30 - 23.50%, in dry mesocarp 30.92 - 41.30% / 41.68 - 50.15% and dry endocarp 55.19 - 76.44% / 91.20 - 92.72%. We determined antioxidant activity in methanolic extracts of the species C. oblonga / P. sinensis in dry exocarp in the range 93.29 - 93.32% / 91.87 - 93.25%, in fresh mesocarp 10.29 - 36.0% / 17.10 - 17.11%, in dry mesocarp 54.55 - 74.11% / 80.39 - 84.11% and in dry endocarp 95.14 - 95.39% / 94.97 - 95.62%. Results document that the fruits of both species can be practically used in the preparation of many dishes, while they can be used as raw material for pharmaceutical and cosmetic use

Redistribution of mineral elements in wheat grain when applying the complex enzyme preparations based on phytase

Biogenic minerals play an important role in the whole human nutrition, but they are included in the grain of the phytates that reduces their bioavailability. Whole wheat bread is generally considered a healthy food, but the presence of mineral elements in it is insignificant, because of weak phytate degradation. From all sources of exogenous phytase the most productive are microscopic fungi. To accelerate the process of transition hard mineral elements are mobilized to implement integrated cellulolytic enzyme preparation based on the actions of phytase (producer is Penicillium canescens). Phytase activity was assessed indirectly by the rate of release of phosphate from the substrate. It has been established that the release rate of the phosphoric acid substrate is dependent on the composition of the drug and the enzyme complex is determined by the presence of xylanase. The presented experimental data shows that a cellulase treatment of the grain in conjunction with the β-glucanase or xylanase leading to an increase in phytase activity could be 1.4 - 2.3 times as compared with the individual enzymes. As a result of concerted action of enzymes complex preparation varies topography grain, increase the pore sizes in seed and fruit shells that facilitate the penetration of the enzyme phytase in the aleurone layer to the site of phytin hydrolysis and leads to an increase in phytase activity. In terms of rational parameters of enzymatic hydrolysis, the distribution of mineral elements in the anatomical parts of the grain after processing complex enzyme preparation with the help of X-ray detector EMF miniCup system in a scanning electron microscope JEOL JSM 6390 were investigated. When processing enzyme preparation wheat trend in the distribution of mineral elements, characteristic of grain - the proportion of these elements in the aleurone layer decreases, and in the endosperm increases. Because dietary fiber and phytate found together in the peripheral layers of fiber-rich grains, it is difficult to separate the effects of degradation processes nonstarch polysaccharides and fiberphytate redistribution of polyvalent metal ions. However, studies have shown that phytase - an effective mechanism for regulating mineral nutrient diet. Application of phytase in grain bakery technology will increase the biological value of the product.  

Morphological characteristics for fruits of aronia mitschurinii A.K. Skvortsov & Maitul.

The aim of this study was to determine morphometric characteristics of fruits within some phenotypes of Aronia mitschurinii A.K. Skvortsov & Maitul. Their morphometric parameters were following: weight from 0.75 g (AM-03) to 1.52 g (AM-04), length from 9.46 mm (AM-03) to 12.73 mm (AM-04), diameter from 10.49 mm (AM-03) to 13.73 mm (AM-04), fruits number in the corymb from 11.33 (AM-07) to 20.13 (AM-03), cumulative weight of fruits in the corymb from 10.42 g (AM-07) to 21.73 g (AM-04), volume of fruits from 0.55 (AM-03) to 1.26 (AM-04) cm3. The shape index of the fruits was found in the range of 0.87 (AR-01, AR-05, AR-07) to 0.93 (AM-02). The analysis of coefficient of variation showed the difference of variability in morphological characteristics between Aronia mitschurinii samples. Data showed that the most variability of important selection characteristics are the average cumulative mass of fruits in a сorymb - from 12.34 (AM-03) to 38.61 (AM-02) % and fruit number of fruits in the сorymb - from 14.56 (AM-03) to 36.88 (AM-02) %. The other characteristics are more or less stable. The introduction population of the Aronia mitschurinii, was created in the M.M. Gryshko National Botanical Garden in Kyiv, has a sufficient potential for successful selection work

Biologically valuable substances in garlic (Allium sativum L.) – A review

Garlic (Allium sativum L.) is one of the most valuable plants in the world because it contains important substances with protective and healing effects on human health. Its health-promoting effects have been already known in ancient Egypt, China, Greece and also the Romans used it. Many studies have shown that garlic can help from colds, coughs, flu, pulmonary diseases, clean blood vessels, lowers blood pressure and cholesterol and has antibacterial, antiviral, anti-carcinogenic, anti-mutagenic and antioxidant properties. Garlic contains antioxidant that support the body´s defense mechanism against oxidative damage. The physiological effect of garlic can be affected by sulphur – containing compounds as well as other biologically active compounds such as polyphenols (mainly flavonoids), minerals (Ca, Fe, I, K, Mg, Na, Zn) and vitamins (A, B1, B2, B6, C). The main sulphur compound in garlic is alliin, converted to allicin by the enzyme alliinase, which results in a characteristic garlic aroma and taste

Morphology and sensory evaluation of traditional products from different landraces of pumpkin (Cucurbita spp.)

The goal of study was analysis of morphological characteristics and organoleptic attributes of traditional food products from the pulp of regional varieties of pumpkin (Cucurbita spp.). For experimental purposes we used a landrace from Ukraine (A) and 5 landraces from Serbia (B, C, D, E and F). For the fruit of all landraces were determined by the average weight of fetuses in the range 1025.0 g (F) - 7680.0 g (B), stem weight 3.35 g (F) - 25 g (B), pulp weight 668.15 g (F) - 6351.10 g (B), placental weight of 90.47 g (E) - 515.50 g (B), seed weight 44.55 g (F) - 277.80 g (B) and mass exocarp 124.20 g (E) - 471.70 g (B). The dry matter content of flesh were determined in the range 7.8 to 11.6 %. The total weight of the fruit pulp was determined by weight in the range of 65.19 up to 88.50 % and the proportion of seed weight in the range of 1.65 to 6.58 %. Differences between genotypes were determined by the texture attributes of dry pulp of selected landraces using an electron microscope. For the sensory evaluation by traditional technology, we drafted three food products from the pulp of each landraces and mash, roast flesh, and baked rolls (traditional name strudel). All products have been heat-treated by boiling (slurry at 100 °C) and baked at 200 °C. Organoleptic evaluation of samples, we identified significant differences between rated landrace in perceptions of color, texture, consistency, hardness, juiciness, flavor, aftertaste, swallowed, sweetness, acidity, smell and overall impression. Experimental results of morphological analysis, we evaluated the descriptive statistics and the relationship between the characters were determined by linear correlations. Sensory analysis were evaluated by scaling and ranking methods. In general, we identified the best organoleptic properties in the landraces originating from Serbia (C). The results confirmed significant differences between test landrace in morphological and organoleptic characteristics

Constituents of the essential oil in Solidago canadensis L. from Eurasia

Hydro distilled essential oils in air-dry samples of aerial parts of Solidago canadensis L., (Asteraceae) from eight local invasive populations were investigated by GC-MS analysis. A comparative study on quantity and composition of the essential oils obtained from plants, growing in different ecological and climatic conditions, ontogenesis phase and different plant organs was carried out The major compounds detected in oil samples of S. canadensis were α-pinene (1.3 - 61.27%), limonene (0.5 - 22.5%), bornyl acetat (3.4 - 29.8%) and germacrene D (1.8 - 39.2%). Samples from inflorescences contained the maximal percentage of monoterpene hydrocarbons, while the leaves' samples showed the maximal cumulative percentage of sesquiterpene and monoterpene hydrocarbons. Data obtained from our studies confirm the availability of alien invasive species Solidago canadensis for medicine and many other purposes. The variability of the qualitative and quantitative composition of essential oils in different geographical locations will allow futher selection of form containing the maximum amount of active substances

Morphometric characteristics of sweet chestnut (Castanea sativa Mill.) fruits

Aim of this study was to determine morphometric differences of fruits between selected sweet chestnuts (Castanea sativa Mill.). The 28 genotypes (referred as CS-01 to CS-28) were introduced by seeds from Czech Republic, Carpathians, Kyrgyzstan. Genotypes of sweet chestnut are grow more than 30 years in Forest-Steppe of Ukraine in the M.M. Gryshko National Botanical Garden of NAS of Ukraine. They are well adapted to the climatic and soil conditions. The fruits were collected at the period of their full maturity (September). The population differs in weight, shape, size and color of fruits. Their morphometric parameters were following: weight from 1.70 g (CS-26) to 18.60 g (CS-20), length from 8.07 mm (CS-28) to 33.39 mm (CS-11), width from 16.34 mm (CS-28) to 40.95 mm (CS-11), thickness from 9.02 mm (CS-26) to 28.70 mm (CS-11) and hilum length from 6.62 mm (CS-26) to 31.30 mm (CS-07), hilum width from 6.50 mm (CS-23) to 19.99 mm (CS-07). The shape index of the fruits was found in the range of 0.81 (CS-20) to 0.98 (CS-12). The shape index of the hilum was found in the range of 1.48 (CS-04) to 2.03 (CS-23). The outcome of the research point to the fact that the genepool Ukrainian sweet chestnut is a rich source of genetic diversity and might be used in selection for creation a new genotypes and cultivars