Macroporous polymers tailored as supports for large biomolecules: Ionic liquids as porogenic solvents and as surface modifiers

Highly ordered rod-like polymeric monoliths with large-pores have been successfully synthesized using ionic liquids (ILs) IL-1 (1-Butyl-3-methylimidazolium bis (trifluoromethylsulfonyl)imide [BMIM][NTf2]) and IL-2 (1-octyl-3-methylimidazolium bis (trifluoromethylsulfonyl)imide [OMIM][NTf2]) as alternative porogenic solvents. The presence of ILs can not only promote the formation of a highly ordered macroporous structure, control the morphology of the polymer and control the chemical composition of surfaces for monoliths prepared from DVB. In this regard, post-functionalization of the monoliths can be easily achieved using the functional monomers introduced in the polymerization process or the unreacted vinyl groups present in the polymeric matrix. This control has allowed the preparation of monolithic Supported Ionic Liquid-like Phases (m-SILLPs) with excellent morphological properties. These m-SILLPs have been studied as supports for large biomolecules. Bioadsorption studies show that the adsorbed amount of protein reaches values as high as 150–200 mg of protein per gram of support

Rose Bengal Immobilized on Supported Ionic‐Liquid‐like Phases: An Efficient Photocatalyst for Batch and Flow Processes

This is the peer reviewed version of the following article: Rose Bengal Immobilized on Supported Ionic‐Liquid‐like Phases: An Efficient Photocatalyst for Batch and Flow Processes, which has been published in final form at https://doi.org/10.1002/cssc.201901533. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.The catalytic activity of Rose Bengal (RB) immobilized on supported ionic liquid (IL)‐like phases was evaluated as a polymer‐supported photocatalyst. In these systems, the polymer was designed to play a pivotal role. The polymeric backbone adequately modified with IL‐like moieties (supported IL‐like phases, SILLPs) was not just an inert support for the dye but controlled the accessibility of reagents/substrates to the active sites and provided specific microenvironments for the reaction. The structure of SILLPs could be finetuned to adjust the catalytic efficiency of the RB‐SILLP composites, achieving systems that were more active and stable than the related systems in the absence of IL‐like units

Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beads

Lipase B from Candida antarctica (CALB) is able to catalyze C–C bond formation. After immobilization onto a hydrophobic PS-DVB support, the activity increases when compared to that of the soluble or tan – the commercially available Novozyme 435 (being up to 6 fold more active). Our results show that although this activity is not related to the catalytic group, the promiscuous activity of CALB may be tuned via immobilization. In addition, we have show that the secondary structure of both immobilized enzymes is quite different, using FT-ATR-IR spectroscopy

An efficient microwave-assisted enzymatic resolution of alcohols using a lipase immobilised on supported ionic liquid-like phases (SILLPs)

The combined action of microwave irradiation and CALB immobilised onto polymeric SILLPs leads to an excellent activity improvement (28 fold) and operational stability towards reuse (12 operation cycles). This procedure also allows the microwave-assisted one-pot DKR by the combination with a solid acid catalyst