34 research outputs found
Role of the cytoplasmic isoform of RBFOX1/A2BP1 in establishing the architecture of the developing cerebral cortex
Stimulation of the stress-induced expression of stress proteins by curcumin in cultured cells and in rat tissues in vivo
MUNC18–1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development
Abstract While Munc18–1 interacts with Syntaxin1 and controls the formation of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) complex to regulate presynaptic vesicle fusion in developed neurons, this molecule is likely to be involved in brain development since its gene abnormalities cause early infantile epileptic encephalopathy with suppression-burst (Ohtahara syndrome), neonatal epileptic encephalopathy and other neurodevelopmental disorders. We thus analyzed physiological significance of Munc18–1 during cortical development. Munc18–1-knockdown impaired cortical neuron positioning during mouse corticogenesis. Time-lapse imaging revealed that the mispositioning was attributable to defects in radial migration in the intermediate zone and cortical plate. Notably, Syntaxin1A was critical for radial migration downstream of Munc18–1. As for the underlying mechanism, Munc18–1-knockdown in cortical neurons hampered post-Golgi vesicle trafficking and subsequent vesicle fusion at the plasma membrane in vivo and in vitro, respectively. Notably, Syntaxin1A-silencing did not affect the post-Golgi vesicle trafficking. Taken together, Munc18–1 was suggested to regulate radial migration by modulating not only vesicle fusion at the plasma membrane to distribute various proteins on the cell surface for interaction with radial fibers, but also preceding vesicle transport from Golgi to the plasma membrane. Although knockdown experiments suggested that Syntaxin1A does not participate in the vesicle trafficking, it was supposed to regulate subsequent vesicle fusion under the control of Munc18–1. These observations may shed light on the mechanism governing radial migration of cortical neurons. Disruption of Munc18–1 function may result in the abnormal corticogenesis, leading to neurodevelopmental disorders with MUNC18–1 gene abnormalities
Expression of Drebrin, an actin binding protein, in basal cell carcinoma, trichoblastoma and trichoepithelioma
Drebrin, an F-actin binding protein, is known
to play important roles in cell migration, synaptogenesis
and neural plasticity. Although drebrin was long thought
to be specific for neuronal cells, its expression has
recently been reported in non-neuronal cells. As for
skin-derived cells, drebrin was shown to be enriched at
adhering junctions (AJs) in cultured primary
keratinocytes and also be highly expressed in basal cell
carcinoma (BCC) cells. Since BCC and two types of
benign neoplasm, trichoblastoma and trichoepithelioma,
are considered to derive from the same origin, follicular
germinative cells, it is sometimes difficult to morphologically
distinguish BCC from trichoblastoma and
trichoepithelioma. In this study, we performed
immunohistochemical staining of drebrin in BCC,
trichoblastoma and trichoepithelioma, to examine
whether drebrin could serve as a biomarker for BCC
diagnosis. In western blotting, drebrin was detected
highly and moderately in the lysates from a squamous
cell carcinoma cell line, DJM-1, and normal human
epidermis, respectively. In immunofluorescence
analyses, drebrin was colocalized with markers of AJs
and tight junctions in DJM-1 cells and detected at cellcell
junction areas of human normal epidermis tissue.
We then examined the distribution patterns of drebrin in
BCC, trichoblastoma and trichoepithelioma. In BCC
tissues, intense and homogeneous drebrin expression
was observed mainly at tumor cell-cell boundaries. In
contrast, drebrin was stained only weakly and nonhomogeneously
in trichoblastoma and trichoepthelioma
tissue samples. For differential diagnosis of BCC,
drebrin may be a novel and useful marker
L'Auto-vélo : automobilisme, cyclisme, athlétisme, yachting, aérostation, escrime, hippisme / dir. Henri Desgranges
14 mars 19391939/03/14 (A40,N13962)
Identification and Characterization of Nuclear Pore Complex Components in Arabidopsis thaliana[W][OA]
Interactive proteomics technology was used to identify Arabidopsis nucleoporins, which are components of the nuclear pore complex (NPC). Nucleoporin domain organization is similar in plants, vertebrates, and yeast. This finding suggests that most NPC structures are conserved throughout eukaryotes