INVESTIGATION ON MORPHOLOGICAL TRAITS ON LEAF, POD AND SEED OF LOCAL BEAN (Phaseolus vulgaris L.) POPULATIONS IN MACEDONIA

In this research there were analyzed forty-four (44) local bean populations from ssp.nanus (dwarf), sown on open field in randomized complete block design during 2008 at Institute of Agriculture, Skopje. Tested varieties were compared with two Slovenian varieties used as check variety. The objective was to identify some morphological parameters on leaf, pod and seed of these bean populations. The whole examined material was collected from eastern part of Macedonia from Malesregion, precisely from Pehchevo. Characterization and the determination of genetic variability were described according to the list of descriptors for beans of the International Commission on Genetic Resources– IBPGR (International Board for Plant Genetic Resources). Generally, all tested varieties showed differences in terms of the examined traits. Approximately half of the analyzed populations had a green leaf with a triangular shape and rare fibrous. The average values for length/ width of leaf range from 7.02-11.17cm and 4.59-8.16cm. The length and width traits of the leaf showed relatively low variability from 12.72% to 13%. In the analyzed collection the most of the landraces had a green color of pod, curved legumes, with round-oval and pear-shaped cross-section.&nbsp

YIELD AND YIELD COMPONENTS ON SOME WHEAT VARIETIES GROWN IN ALEKSINAC REGION

Yield and yield components of 5 wheat varieties (Kruna, Toplica, Zvezdana, Etida, Angelina) in Aleksinac region (Serbia) were analysed. The experiment was performed in randomized block design in 3 replications on the experimental field in area of Aleksinac city. The results showed relatively high yields in all varieties. The general average yield was 6140 kg ha-1. The highest average yield varied from 6858 kg ha-1 in cultivar Zvezdana to 5050 kg ha-1 in Toplica. The cultivar Kruna showed lowest number of productive stems per square meter – 572, and largest number in Zvezdana and Angelina, with an average of 658 and 641, respectively. Average longest spike was found in variety Angelina 12.4 cm, and shortest in Toplica (9.8 cm). The average number of spikelets per spike for all cultivars was 19.4. Cultivar Angelina showed biggest number (21.1), and Kruna smallest number (17.9). The biggest number of grains per spike was obtained in cultivar Etida (54.8), and lowest in Zvezdana (51.4). During the examination, the highest average value for hectoliter weight is obtained in variety Zvezdana (78.3 kg hl-1), and lowest in Toplica (75.4 kg hl-1). From the data on yield and yield components, it can be concluded that all tested varieties can be grown in the region of Aleksinac, with preference to varieties Zvezdana and Etida

A fast and robust hepatocyte quantification algorithm including vein processing

<p>Abstract</p> <p>Background</p> <p>Quantification of different types of cells is often needed for analysis of histological images. In our project, we compute the relative number of proliferating hepatocytes for the evaluation of the regeneration process after partial hepatectomy in normal rat livers.</p> <p>Results</p> <p>Our presented automatic approach for hepatocyte (HC) quantification is suitable for the analysis of an entire digitized histological section given in form of a series of images. It is the main part of an automatic hepatocyte quantification tool that allows for the computation of the ratio between the number of proliferating HC-nuclei and the total number of all HC-nuclei for a series of images in one processing run. The processing pipeline allows us to obtain desired and valuable results for a wide range of images with different properties without additional parameter adjustment. Comparing the obtained segmentation results with a manually retrieved segmentation mask which is considered to be the ground truth, we achieve results with sensitivity above 90% and false positive fraction below 15%.</p> <p>Conclusions</p> <p>The proposed automatic procedure gives results with high sensitivity and low false positive fraction and can be applied to process entire stained sections.</p

Anisotropic colloids through non-trivial buckling

We present a study on buckling of colloidal particles, including experimental, theoretical and numerical developments. Oil-filled thin shells prepared by emulsion templating show buckling in mixtures of water and ethanol, due to dissolution of the core in the external medium. This leads to conformations with a single depression, either axisymmetric or polygonal depending on the geometrical features of the shells. These conformations could be theoretically and/or numerically reproduced in a model of homogeneous spherical thin shells with bending and stretching elasticity, submitted to an isotropic external pressure.Comment: submitted to EPJ

Biodegradable polymer‐Metal‐Organic Framework (MOF) composites for controlled and sustainable pesticide delivery

Due to high surface area, loading capacity, and selectivity, Metal-Organic Frameworks (MOFs) have shown much promise recently for potential applications in extraction and delivery of agrochemicals for environmental remediation and sustainable release, respectively. However, application of MOFs for pesticide delivery in wider agricultural context can be restricted by their granular form. Herein, an alternative approach is studied using biodegradable polymer-MOF composites to address this limitation. The loading and release of a widely used pesticide, 2,4-dichloropheoxycetic acid (2,4-D), is studied using two MOFs, UiO-66 and UiO-66-NH2, and the 2,4-D-loaded MOFs are incorporated into biodegradable polycaprolactone composites for convenient handling and minimizing runoff. The MOFs are loaded by in-situ, and post-synthetic methods, and characterised thoroughly to ensure successful synthesis and loading of 2,4-D. The pesticide release studies are performed on the MOFs and composites in distilled water, and analysed using UV-Vis spectroscopy, demonstrating sustained-release of 2,4-D over 16 days. The loaded MOF samples show high loading capacity, with up to 45 wt% for the in-situ loaded UiO-66. Release kinetics show more sustained release of 2,4-D from UiO-66-NH2 compared to UiO-66, which can be due to supramolecular interactions between the NH2 group of UiO-66-NH2 and 2,4-D. This is further supported by computational studies

Dynamics of non-spherical dust in the coma of 67P/Churyumov- Gerasimenko constrained by GIADA and ROSINA data

Among the comet 67P/Churyumov-Gerasimenko (67P/C-G) in situ measurements, the closest that have ever been performed at a comet nucleus, are also those of speed, mass, and cross-section of cometary grains performed by the Grain Impact Analyser and Dust Accumulator (GIADA) instrument. To interpret GIADA data, we performed dust dynamical numerical simulations with both spherical and non-spherical (spheroids) shapes. This allowed us to analyse how the grain non-sphericity affects the data interpretation. We find that some measured dust speeds are unlikely reproducible when a spherical shape is considered. We considered two GIADA observational periods, 2015 February 19-27 and 2015 March 13-28. Gas parameters calibrated with the Rosetta Orbiter Spectrometer for Ion and Neutral Analysis (ROSINA) measurements have been used to retrieve the gas conditions to set up the dust particle motion. The dust grains are assumed to be out of the near nucleus coma, i.e. where the gas velocity is radial and constant, therefore they are either aligned or have random but constant orientation with respect to the gas drag. We reproduced the GIADA dust speeds, using spheres and two different spheroidal shapes. We find that the particle shapes that reproduce best the GIADA dust speeds are consistent with the particle shape constrained by the GIADA data. We obtain different terminal velocities for spherical and non-spherical particles of the same mass. The shape, which reproduces the GIADA data, is oblate rather than prolate spheroid. We obtain rotational frequencies of the spheroidal particles that best fit the GIADA measurements in these periods

Ionizing Radiation-Induced Oxidative Stress Alters miRNA Expression

). treatment, and 45 after etoposide treatment. Substantial overlap between the miRNA expression changes between agents was observed suggesting a signature miRNA response to cell stress. Changes in the expression of selected miRNA species varied in response to radiation dose and time. Finally, production of reactive oxygen species (ROS) increased with increasing doses of radiation and pre-treatment with the thiol antioxidant cysteine decreased both ROS production and the miRNA response to radiation., and etoposide. Additionally, pre-treatment with cysteine prevented radiation-induced alterations in miRNA expression which suggests that miRNAs are responsive to oxidative stress. Taken together, these results imply that miRNAs play a role in cellular defense against exogenous stress and are involved in the generalized cellular response to genotoxic oxidative stress

The Meiotic Recombination Checkpoint Suppresses NHK-1 Kinase to Prevent Reorganisation of the Oocyte Nucleus in Drosophila

The meiotic recombination checkpoint is a signalling pathway that blocks meiotic progression when the repair of DNA breaks formed during recombination is delayed. In comparison to the signalling pathway itself, however, the molecular targets of the checkpoint that control meiotic progression are not well understood in metazoans. In Drosophila, activation of the meiotic checkpoint is known to prevent formation of the karyosome, a meiosis-specific organisation of chromosomes, but the molecular pathway by which this occurs remains to be identified. Here we show that the conserved kinase NHK-1 (Drosophila Vrk-1) is a crucial meiotic regulator controlled by the meiotic checkpoint. An nhk-1 mutation, whilst resulting in karyosome defects, does so independent of meiotic checkpoint activation. Rather, we find unrepaired DNA breaks formed during recombination suppress NHK-1 activity (inferred from the phosphorylation level of one of its substrates) through the meiotic checkpoint. Additionally DNA breaks induced by X-rays in cultured cells also suppress NHK-1 kinase activity. Unrepaired DNA breaks in oocytes also delay other NHK-1 dependent nuclear events, such as synaptonemal complex disassembly and condensin loading onto chromosomes. Therefore we propose that NHK-1 is a crucial regulator of meiosis and that the meiotic checkpoint suppresses NHK-1 activity to prevent oocyte nuclear reorganisation until DNA breaks are repaired

miR-17–92 cluster: ups and downs in cancer and aging

The miR-17–92 cluster encoding 6 single mature miRNAs was identified a couple of years ago to contain the first oncogenic miRNAs. Now, one of these 6 miRNAs, miR-19 has been identified as the key responsible for this oncogenic activity. This in turn reduces PTEN levels and in consequence activates the AKT/mTOR pathway that is also prominently involved in modulation of organismal life spans. In contrast, miR-19 and other members of the miR-17–92 cluster are found to be commonly downregulated in several human replicative and organismal aging models. Taken together, these findings suggest that miR-19 and the other members of the miR-17–92 cluster might be important regulators on the cross-roads between aging and cancer. Therefore, we here briefly summarize how this cluster is transcriptionally regulated, which target mRNAs have been confirmed so far and how this might be linked to modulation of organismal life-spans