Recombinant spidroins from infinite circRNA translation

Spidroins are a diverse family of peptides and the main components of spider silk. They can be used to produce sustainable, lightweight and durable materials for a large variety of medical and engineering applications. Spiders’ territorial behaviour and cannibalism precludes farming them for silk. Recombinant protein synthesis is the most promising way of producing these peptides. However, many approaches have been unsuccessful in obtaining large titres of recombinant spidroins or ones of sufficient molecular weight. The work described here is focused on expressing high molecular weight spidroins from short circular RNA molecules. Mammalian host cells were transfected with designed circular-RNA-producing plasmid vectors. A backsplicing approach was implemented to successfully circularise RNA in a variety of mammalian cell types. This approach could not express any recombinant spidroins based on a variety of qualitative protein assays. Further experiments investigated the reasons behind this. Additionally, due to the diversity of spidroins in a large number of spider lineages, there are potentially many spidroin sequences left to be discovered. A bioinformatic pipeline was developed that accepts transcriptome datasets from RNA sequencing and uses tandem repeat detection and profile HMM annotation to identify novel sequences. This pipeline was specifically designed for the identification of repeat domains in expressed sequences. 21 transcriptomes from 17 different species, encompassing a wide selection of basal and derived spider lineages, were investigated using this pipeline. Six previously undescribed spidroin sequences were discovered. This pipeline was additionally tested in the context of the suckerin protein family. These proteins have recently been investigated for their potential properties in medicine and engineering including adhesion in wet environments. The computational pipeline was able to double the number of suckerins known to date. Further phylogenetic analysis was implemented to expand on the knowledge of suckerins. This pipeline enables the identification of transcripts that may have been overlooked by more mainstream analysis methods such as pairwise homology searches. The spidroins and suckerins discovered by this pipeline may contribute to the large repertoire of potentially useful properties characteristic of this diverse peptide family

Automated Intelligent Monitoring and the Controlling Software System for Solar Panels

The inspection of the solar panels on a periodic basis is important to improve longevity and ensure performance of the solar system. To get the most solar potential of the photovoltaic (PV) system is possible through an intelligent monitoring & controlling system. The monitoring & controlling system has rapidly increased its popularity because of its user-friendly graphical interface for data acquisition, monitoring, controlling and measurements. In order to monitor the performance of the system especially for renewable energy source application such as solar photovoltaic (PV), data-acquisition systems had been used to collect all the data regarding the installed system. In this paper the development of a smart automated monitoring & controlling system for the solar panel is described, the core idea is based on IoT (the Internet of Things). The measurements of data are made using sensors, block management data acquisition modules, and a software system. Then, all the real-time data collection of the electrical output parameters of the PV plant such as voltage, current and generated electricity is displayed and stored in the block management. The proposed system is smart enough to make suggestions if the panel is not working properly, to display errors, to remind about maintenance of the system through email or SMS, and to rotate panels according to a sun position using the Ephemeral table that stored in the system. The advantages of the system are the performance of the solar panel system which can be monitored and analyzed

Recombinant spidroins from infinite circRNA translation

Spidroins are a diverse family of peptides and the main components of spider silk. They can be used to produce sustainable, lightweight and durable materials for a large variety of medical and engineering applications. Spiders’ territorial behaviour and cannibalism precludes farming them for silk. Recombinant protein synthesis is the most promising way of producing these peptides. However, many approaches have been unsuccessful in obtaining large titres of recombinant spidroins or ones of sufficient molecular weight. The work described here is focused on expressing high molecular weight spidroins from short circular RNA molecules. Mammalian host cells were transfected with designed circular-RNA-producing plasmid vectors. A backsplicing approach was implemented to successfully circularise RNA in a variety of mammalian cell types. This approach could not express any recombinant spidroins based on a variety of qualitative protein assays. Further experiments investigated the reasons behind this. Additionally, due to the diversity of spidroins in a large number of spider lineages, there are potentially many spidroin sequences left to be discovered. A bioinformatic pipeline was developed that accepts transcriptome datasets from RNA sequencing and uses tandem repeat detection and profile HMM annotation to identify novel sequences. This pipeline was specifically designed for the identification of repeat domains in expressed sequences. 21 transcriptomes from 17 different species, encompassing a wide selection of basal and derived spider lineages, were investigated using this pipeline. Six previously undescribed spidroin sequences were discovered. This pipeline was additionally tested in the context of the suckerin protein family. These proteins have recently been investigated for their potential properties in medicine and engineering including adhesion in wet environments. The computational pipeline was able to double the number of suckerins known to date. Further phylogenetic analysis was implemented to expand on the knowledge of suckerins. This pipeline enables the identification of transcripts that may have been overlooked by more mainstream analysis methods such as pairwise homology searches. The spidroins and suckerins discovered by this pipeline may contribute to the large repertoire of potentially useful properties characteristic of this diverse peptide family

Implementation of the Algorithm for Congestion control in the Dynamic Circuit Network (DCN)

Transport Control Protocol (TCP) incast congestion happens when a number of senders work in parallel with the same server where the high bandwidth and low latency network problem occurs. For many data center network applications such as a search engine, heavy traffic is present on such a server. Incast congestion degrades the entire performance as packets are lost at a server side due to buffer overflow, and as a result, the response time becomes longer. In this work, we focus on TCP throughput, round-trip time (RTT), receive window and retransmission. Our method is based on the proactive adjust of the TCP receive window before the packet loss occurs. We aim to avoid the wastage of the bandwidth by adjusting its size as per the number of packets. To avoid the packet loss, the ICTCP algorithm has been implemented in the data center network (ToR)

The use of “big data” in the organization of repair and construction works to ensure OTR

Organization of work takes a significant place among the basic tasks of the construction industry. The organization of repair and construction works (R-CW) is especially important, since they have specific features in comparison with new construction. Today, information technologies are actively developing, which make significant changes in various industries, including construction. One of the progressive technologies today is the analysis of “big data”. This article outlines the basic concept of using “big data” in the organization of R-CW, which makes it possible to increase the efficiency of organizational decisions, based on the characteristics of a particular repair and construction organization. The methodology proposed by the authors, aimed at the implementation of this concept, includes the solution of a number of tasks, such as assessing organizational decisions that are developed within the framework of a work production project, determining the level of organizational and technological reliability (OTR) and, as a consequence, choosing the best solution. As a tool for the implementation of the above tasks, the authors use a simulation model that makes it possible to simulate the process of performing repair and construction work, taking into account the random nature of their duration. The authors describe the experience of using the proposed methodology in the practice of functioning of one of the Russian construction organizations

A Survey on Simultaneous Wireless Information and Power Transfer

This paper presents a comprehensive study related to simultaneous wireless information and power transfer (SWIPT) in different types of wireless communication setups. Harvesting energy using SWIPT is an appealing solution in the context of extending battery life of wireless devices for a fully sustainable communication system. Strong signal power increases power transfer, but also causes more interference in information transfer, causing realization of the SWIPT challenging problem. This article provides an overview of technical evolution of SWIPT. A survey and qualitative comparison of the existing SWIPT schemes is provided to demonstrate their limitations in the current and 5G networks. Open challenges are emphasized and guidelines are provided to adapt the existing schemes in order to overcome these limitations and make them fit for integrating with the modern and emerging next generation communication networks, such as 5G systems

Development of a Metal Cutting Tool Fase in Order to Create the Conditions of Ringed Chips Wrapping

When processing ductile metals with high cutting speed, there is a need to take additional measures for a comfortable and safe formation and removal of chips. In the conditions of large-scale manufacture, it is recommended to produce flow chips in the form of short fragments, while in the conditions of small-lot and single-piece manufacture, it is reasonable to wrap the chips spirally with a rather small turn radius. Such way of chips formation reduces the time of its removal from the working area as well as facilitates its transportation and processing. In order to solve the problem of chip wrapping and breakage, almost all modern manufacturers of tools with replaceable many-sided plates (RMSP) followed the way of complication of tool faces and determination of the areas of effective chip breaking. On the one hand, the suggested solution turns out to be effective; however, as showed the analysis of recommended cutting modes for complex forms of RMSP made by leading manufacturers, they all correspond to the definite cross section of the cut-layer S/t=0.1

Temperature-Induced Structural Transformations in Undoped and Eu3+-Doped Ruddlesden–Popper Phases Sr2SnO4 and Sr3Sn2O7: Relation to the Impedance and Luminescence Behaviors

We report that luminescence of Eu3+ ion incorporated into Ruddlesden–Popper phases allows monitoring phase transition in powders (instead of single crystals), in a time-efficient manner (compared to neutron diffraction), and importantly, with greater sensitivity than previous methods. Crystal structure and dielectric response of undoped and 0.5%Eu3+-doped Sr3Sn2O7 ceramics were studied as a function of temperature over the temperature range of 300–800 K. The luminescence studies of 0.5%Eu3+-doped Sr2SnO4 and Sr3Sn2O7 samples were performed in the temperature range of 80–500 K. These results were compared with the respective dependences for the undoped compounds. The structural transformations in 0.5%Eu3+-doped Sr3Sn2O7 were found at 390 and 740 K. The former is associated with the isostructural atomic rearrangement that resulted in a negative thermal expansion along two of three orthorhombic crystallographic axes, while the latter corresponds to the structural transition from the orthorhombic Amam phase to the tetragonal I4/mmm one. A similar temperature behavior with the structural transformations in the same temperature ranges was observed in undoped Sr3Sn2O7, although the values of lattice parameters of the Eu3+-doped and undoped compounds were found to be slightly different indicating an incorporation of europium in the crystal lattice. A dielectric anomaly associated with a structural phase transition was observed in Sr3Sn2O7 at 390 K. Optical measurements performed over a wide temperature range demonstrated a clear correlation between structural transformations in Eu3+-doped Sr2SnO4 and Sr3Sn2O7 and the temperature anomalies of their luminescence spectra, suggesting the efficacy of this method for the determination of subtle phase transformations

Dielectric properties of Bi-substituted LDHs synthesized by co-precipitation and sol-gel methods

Magnesium-aluminum-bismuth layered double hydroxides (Mg3Al1-xBix; LDHs) were prepared using both coprecipitation and sol-gel methods. For the preparation of Mg/Al/Bi LDH by the co-precipitation method, the appropriate amounts of dissolved starting materials (Al(NO3)3 · 9H2O, Mg(NO3)2 · 6H2O and Bi(NO3)3 · 5H2O) were mixed with a solution of NaHCO3:NaOH. In the sol-gel processing, the precursor Mg-Al-Bi-O gels were synthesized using the same starting materials and ethylene glycol as complexing agent. The mixed-metal oxides obtained by subsequent heating of Mg-Al-Bi-O gels at 650 °C were reconstructed to Mg3Al1-xBix LDHs in water at 80 °C. All the synthesized products were characterized by X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM) and dielectric measurements.publishe

The research of the quality of ECG recording by capacitive electrodes in violation of the skin-electrode contact

This paper presents actual development in the field of personal telemedicine. For personal cardiographs most suitable dry electrodes that do not require a conductive gel. This article describes experiments with capacitive electrodes from Plessey Semiconductors. On the basis of the obtained experimental data, it is planned to create own capacitive electrodes with the possibility of detuning from the skin-electrode contac