18 research outputs found
Identifiable Acetylene Features Predicted for Young Earth-like Exoplanets with Reducing Atmospheres Undergoing Heavy Bombardment
The chemical environments of young planets are assumed to be largely influenced by the impacts of bodies lingering on unstable trajectories after the dissolution of the protoplanetary disk. We explore the chemical consequences of impacts within the context of reducing planetary atmospheres dominated by carbon monoxide, methane, and molecular nitrogen. A terawatt high-power laser was selected in order to simulate the airglow plasma and blast wave surrounding the impactor. The chemical results of these experiments are then applied to a theoretical atmospheric model. The impact simulation results in substantial volume mixing ratios within the reactor of 5% hydrogen cyanide (HCN), 8% acetylene (C2H2), 5% cyanoacetylene (HC3N), and 1% ammonia (NH3). These yields are combined with estimated impact rates for the early Earth to predict surface boundary conditions for an atmospheric model. We show that impacts might have served as sources of energy that would have led to steady-state surface quantities of 0.4% C2H2, 400 ppm HCN, and 40 ppm NH3. We provide simulated transit spectra for an Earth-like exoplanet with this reducing atmosphere during and shortly after eras of intense impacts. We predict that acetylene is as observable as other molecular features on exoplanets with reducing atmospheres that have recently gone through their own "heavy bombardments," with prominent features at 3.05 and 10.5 μm
Differential Expression of Non-Coding RNAs and Continuous Evolution of the X Chromosome in Testicular Transcriptome of Two Mouse Species
BACKGROUND: Tight regulation of testicular gene expression is a prerequisite for male reproductive success, while differentiation of gene activity in spermatogenesis is important during speciation. Thus, comparison of testicular transcriptomes between closely related species can reveal unique regulatory patterns and shed light on evolutionary constraints separating the species. METHODOLOGY/PRINCIPAL FINDINGS: Here, we compared testicular transcriptomes of two closely related mouse species, Mus musculus and Mus spretus, which diverged more than one million years ago. We analyzed testicular expression using tiling arrays overlapping Chromosomes 2, X, Y and mitochondrial genome. An excess of differentially regulated non-coding RNAs was found on Chromosome 2 including the intronic antisense RNAs, intergenic RNAs and premature forms of Piwi-interacting RNAs (piRNAs). Moreover, striking difference was found in the expression of X-linked G6pdx gene, the parental gene of the autosomal retrogene G6pd2. CONCLUSIONS/SIGNIFICANCE: The prevalence of non-coding RNAs among differentially expressed transcripts indicates their role in species-specific regulation of spermatogenesis. The postmeiotic expression of G6pdx in Mus spretus points towards the continuous evolution of X-chromosome silencing and provides an example of expression change accompanying the out-of-the X-chromosomal retroposition
Blue and Red Light Modulates SigB-Dependent Gene Transcription, Swimming Motility and Invasiveness in Listeria monocytogenes
Background: In a number of gram-positive bacteria, including Listeria, the general stress response is regulated by the alternative sigma factor B (SigB). Common stressors which lead to the activation of SigB and the SigB-dependent regulon are high osmolarity, acid and several more. Recently is has been shown that also blue and red light activates SigB in Bacillus subtilis. Methodology/Principal Findings: By qRT-PCR we analyzed the transcriptional response of the pathogen L. monocytogenes to blue and red light in wild type bacteria and in isogenic deletion mutants for the putative blue-light receptor Lmo0799 and the stress sigma factor SigB. It was found that both blue (455 nm) and red (625 nm) light induced the transcription of sigB and SigB-dependent genes, this induction was completely abolished in the SigB mutant. The blue-light effect was largely dependent on Lmo0799, proving that this protein is a genuine blue-light receptor. The deletion of lmo0799 enhanced the red-light effect, the underlying mechanism as well as that of SigB activation by red light remains unknown. Blue light led to an increased transcription of the internalin A/B genes and of bacterial invasiveness for Caco-2 enterocytes. Exposure to blue light also strongly inhibited swimming motility of the bacteria in a Lmo0799- and SigB-dependent manner, red light had no effect there. Conclusions/Significance: Our data established that visible, in particular blue light is an important environmental signal with an impact on gene expression and physiology of the non-phototrophic bacterium L. monocytogenes. In natural environments these effects will result in sometimes random but potentially also cyclic fluctuations of gene activity, depending on the light conditions prevailing in the respective habitat
An immature B cell population from peripheral blood serves as surrogate marker for monitoring tumor angiogenesis and anti-angiogenic therapy in mouse models
Molekulární struktura organické hmoty v písčito-hlinité půdě s diferencovaným vláhovým režimem
Prebiotic synthesis initiated in formaldehyde by laser plasma simulating high-velocity impacts
Context. It is well known that hydrogen cyanide and formamide can universally be considered as key molecules in prebiotic synthesis. Despite the fact that formamide has been detected in interplanetary and interstellar environments, other prebiotic species are far more abundant, including, for example, formaldehyde. However, several results indicate that formamide can play the role of important intermediate as well as that of a feedstock molecule in chemical abiogenesis. Diverse recently proposed scenarios of the origins of the first biopolymers show that liquid formamide environments could have been crucial for the formation of nucleobases, nucleosides, and for phosphorylation reactions, which lead to nucleotides.
Aims. Here we report on a wide exploration of the formaldehyde reaction network under plasma conditions mimicking an asteroid descent in an Earth-like atmosphere and its impact.
Methods. Dielectric breakdown using a high-power kJ-class laser system (PALS – Prague Asterix Laser System) along with quantum mechanical, ab initio molecular dynamics, and enhanced sampling simulations have been employed in order to mimic an asteroid impact plasma.
Results. Being more abundant than formamide both in interstellar and interplanetary environments, during the era of early and late heavy bombardment of Earth and other planets, formaldehyde might have been delivered on asteroids to young planets. In the presence of nitrogen-bearing species, this molecule has been reprocessed under plasma conditions mimicking the local environment of an impacting body. We show that plasma reprocessing of formaldehyde leads to the formation of several radical and molecular species along with formamide.
Conclusion. All the canonical nucleobases, the simplest amino acid (i.e., glycine), and the sugar ribose, have been detected after treatment of formaldehyde and nitrogen gas with dielectric breakdown. Our results, supported by quantum mechanical and enhanced sampling simulations, show that formaldehyde – by producing inter alia formamide – may have had the role of starting substance in prebiotic synthesis