The Roles Of Sphingosine-1-Phosphate (S1p) On Arthritis: Review Article

Arthritis is a pathological condition that results in degeneration of the joints. RA and OA are the most common types of arthritis. RA is a chronic joint inflammation caused by the immune system's self-attack on tissues. By contrast, OA is chronic joint inflammation caused by cartilage breakdown. Both illnesses cause joint discomfort, stiffness, and edema. One genetic factor in arthritis is sphingosine-1-phosphate (S1P). S1P regulates bone homeostasis and growth using 5 receptors: 1, 2, 3, 4, and 5. Thus, this literature review intends to investigate the impact of S1P on arthritis. Methods: The eligibility criteria comprised cross-sectional studies that were published in English until January 2024 and exclusively addressed the role of S1P in arthritis. Results and discussion: From 396 publications, 17 relevant articles were located, and 6 were chosen for the review. The S1P/S1P2 signaling pathway releases osteoclasts to degrade cartilage in osteoarthritis. S1P also promotes bone growth by differentiating osteoblasts and forming blood vessels in the bone marrow. The miR-25 rs41274221 polymorphism may reduce osteoporosis risk. IL-6 is also produced by osteoblasts with S1P. Osteoarthritis is associated with elevated blood S1P and MMP-3 levels. Cyclic strain and inflammation increase Sphk1 upregulation and S1P release, suggesting a role in osteoarthritis. In conclusion, the expression of S1P by osteoclasts, osteoblasts, chondrocytes, and tenocytes is believed to play a crucial role in regulating cell migration and the production of cytokines or chemokines throughout the process of bone formation. Focusing on the S1P pathway may help treat bone and joint diseases

Crosstalk between Fas and S1P1 signaling via NF-kB in osteoclasts controls bone destruction in the TMJ due to rheumatoid arthritis

Rheumatoid arthritis (RA) mainly affects various joints of the body, including the temporomandibular joint (TMJ), and it involves an infiltration of autoantibodies and inflammatory leukocytes into articular tissues and the synovium. Initially, the synovial lining tissue becomes engaged with several kinds of infiltrating cells, including osteoclasts, macrophages, lymphocytes, and plasma cells. Eventually, bone degradation occurs. In order to elucidate the best therapy for RA, a comprehensive study of RA pathogenesis needs to be completed. In this article, we discuss a Fas-deficient condition which develops into RA, with an emphasis on the role of sphingosine 1-phosphate (S1P)/S1P receptor 1 signaling which induces the migration of osteoclast precursor cells. We describe that Fas/S1P1 signaling via NF-kB activation in osteoclasts is a key factor in TMJ-RA severity and we discuss a strategy for blocking nuclear translocation of the p50 NF-kB subunit as a potential therapy for attenuating osteoclastogenesis

QUALITATIVE ANALYSIS OF SANTRI HUSADA CADRES FORMATION AND ORAL DENTAL EXAMINATION AT ASSHODIQIYAH ISLAMIC BOARDING SCHOOL SEMARANG

Background: Dental and oral health problems in Indonesia are still high due to the low number of health workers, both dentists and dental nurses. Therefore, the prevention and promotion of dental and oral health services must be improved formation of dental health cadres in a community. Islamic boarding schools are places of Islamic learning where students or santri live permanently and study together with teachers or Kiai. In a pesantren learning environment, it is essential to pay attention to health, especially the condition of the teeth and mouth, so as not to interfere with the learning process. This community service aims to form cadres and carry out dental and oral health checks by dentists and dental co-operative students, Faculty of Dentistry, Sultan Agung Islamic University (UNISSULA) Semarang.Method: In addition, this activity also aims to change students' behaviour thus they put more attention of the condition of their oral cavity and know the importance dental and oral care treatments according to their oral condition. With direct counselling provided by dentists, the cadres gain understanding and knowledge about the oral cavity, including the arrangement of teeth, various kinds of dental and oral problems, and how to treat them. In addition, the cadres also received training on how to brush their teeth with the right brush and toothpaste provided by the Co-assistant of dental students.Result: With the formation of 12 cadres, it is suggested that promotive, preventive, and referral activities for dental and oral health problems in the Asshodiqiyah Islamic boarding school can continue to run to reduce dental and oral health problems.Conclusion: Cadre activities for students and brushing teeth together can increase knowledge in the field of dental and oral health and the ability to clean the mouth teeth of PP students. Asshodiqiyah. This activity was also able to increase the awareness of the students to be more active in caring for and maintaining the health of their teeth and mouth

Potential Role of Rebamipide in Osteoclast Differentiation

Background: Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease that involves changes in subchondral bone and progressive degradation of cartilage. Currently, rebamipide, a gastroprotective drug, is administered to protect gastric mucosa and accelerate ulcer healing. Objectives: Recent studies have shown that rebamipide also attenuates cartilage degeneration by suppressing oxidative damage and inducing homeostasis of the extracellular matrix of articular chondrocytes. Regarding the latter, reduced expression of cathepsin K, NFATc1, c-Src, and integrin β3, and increased expression of nuclear factor-kappa B, have been found to be mediated by the transcription factor, receptor activator of nuclear factor kappa-B ligand (RANKL). Methods: Treatment with rebamipide was also found to activate, mitogen-activated protein kinases such as p38, ERK, and JNK to reduce osteoclast differentiation. Taken together, these results strongly indicate that rebamipide mediates inhibitory effects on cartilage degradation and osteoclastogenesis in TMJ-OA. Results and Conclusion: Here, we highlight recent evidence regarding the potential for rebamipide to affect osteoclast differentiation and TMJ-OA pathogenesis. We also discuss the potential role of rebamipide to serve as a new strategy for the treatment of TMJ-OA

MILK CONSUMPTION AFFECTS THE EXPRESSION OF AMELOGENIN IN AMELOBLAST CELLS DURING AMELOGNESIS (IN VIVO ANALYSIS OF PREGNANT MICE)

Background: Tooth development during embryonic period is complex process that needs enough nutrition for the formation of healthy dental tissue. Amelogenin are proteins that are secreted by ameloblast in the secretory stage that are importants in the crystal formation and the regulation of email thickness. Milk consumption during pregnancy can increase the expression of amelogenin of ameloblas cell in the tooth development process. This research aims to determine the impact of milk during pregnancy against the expression of amelogenin protein of ameloblast cell in the amelogenesis.Method: This is a true experimental laboratories research using post test only control group design. A total of 12 pregnant female mice (Mus Musculus L.) are used and divided into 2 groups, control group (given sterile aquades) and treatment group (given pregnancy milk). The measurements of amelogenin expression are done in 18th day of pregnancy period. Data were analyzed using Independent sampel of T-test analysis.Result: The result of this study showed that the average amounts of amelogenin expression in the treatment group are lower than the control group. Independent sampel t-test showed that there are significant difference in the amount of amelogenin expression between the control and treatment group (P>0,05)Conclusion: The milk consumption during pregnancy influenced the amelogenin expression of ameloblast cell in the mice fetus’s amelogenesis

ACTIVE EDUCATIONAL METHOD THROUGH THE GAMES OF THE TEMPOROMANDIBULAR JOINT’S TO IMPROVE KNOWLEDGE OF THE MARGASARI COMMUNITY RELATED TO JAW JOINT DISORDERS

Background: This TMJ problem or jaw joint disorder is classified as an emergency, so treatment must be done immediately. The causes of this jaw joint problem are multifactorial. Generally divided into two, namely structural disorders and functional disorders. Functional disorders arise due to deviant function due to abnormalities in the position or function of the teeth and chewing muscles. Treatment, when someone has TMJ problems, is usually a reduction action that is carried out directly by a doctor. This cannot be done by ordinary people. However, sometimes people self-diagnose and resort to invalid sources which can actually lead to more serious problems. Method: The educational method is carried out by active counseling and discussions given to the people of Margasari Village. Then, the community was asked to fill out a questionnaire to assess the effectiveness of delivering material about this TMJ. Result: People who have filled out the questionnaire were found to have a significant difference between before and after being given the material. People become more aware of the material and are expected to be able to apply it in everyday life. Conclusion: There is a significant difference between before and after being given TMJ games

Macrophage Motility in Wound Healing Is Regulated by HIF-1α via S1P Signaling

Accumulating evidence indicates that the molecular pathways mediating wound healing induce cell migration and localization of cytokines to sites of injury. Macrophages are immune cells that sense and actively respond to disturbances in tissue homeostasis by initiating, and subsequently resolving, inflammation. Hypoxic conditions generated at a wound site also strongly recruit macrophages and affect their function. Hypoxia inducible factor (HIF)-1α is a transcription factor that contributes to both glycolysis and the induction of inflammatory genes, while also being critical for macrophage activation. For the latter, HIF-1α regulates sphingosine 1-phosphate (S1P) to affect the migration, activation, differentiation, and polarization of macrophages. Recently, S1P and HIF-1α have received much attention, and various studies have been performed to investigate their roles in initiating and resolving inflammation via macrophages. It is hypothesized that the HIF-1α/S1P/S1P receptor axis is an important determinant of macrophage function under inflammatory conditions and during disease pathogenesis. Therefore, in this review, biological regulation of monocytes/macrophages in response to circulating HIF-1α is summarized, including signaling by S1P/S1P receptors, which have essential roles in wound healing

Macrophage Motility in Wound Healing Is Regulated by HIF-1α via S1P Signaling

Accumulating evidence indicates that the molecular pathways mediating wound healing induce cell migration and localization of cytokines to sites of injury. Macrophages are immune cells that sense and actively respond to disturbances in tissue homeostasis by initiating, and subsequently resolving, inflammation. Hypoxic conditions generated at a wound site also strongly recruit macrophages and affect their function. Hypoxia inducible factor (HIF)-1α is a transcription factor that contributes to both glycolysis and the induction of inflammatory genes, while also being critical for macrophage activation. For the latter, HIF-1α regulates sphingosine 1-phosphate (S1P) to affect the migration, activation, differentiation, and polarization of macrophages. Recently, S1P and HIF-1α have received much attention, and various studies have been performed to investigate their roles in initiating and resolving inflammation via macrophages. It is hypothesized that the HIF-1α/S1P/S1P receptor axis is an important determinant of macrophage function under inflammatory conditions and during disease pathogenesis. Therefore, in this review, biological regulation of monocytes/macrophages in response to circulating HIF-1α is summarized, including signaling by S1P/S1P receptors, which have essential roles in wound healing

Macrophage Motility in Wound Healing Is Regulated by HIF-1α via S1P Signaling

Accumulating evidence indicates that the molecular pathways mediating wound healing induce cell migration and localization of cytokines to sites of injury. Macrophages are immune cells that sense and actively respond to disturbances in tissue homeostasis by initiating, and subsequently resolving, inflammation. Hypoxic conditions generated at a wound site also strongly recruit macrophages and affect their function. Hypoxia inducible factor (HIF)-1α is a transcription factor that contributes to both glycolysis and the induction of inflammatory genes, while also being critical for macrophage activation. For the latter, HIF-1α regulates sphingosine 1-phosphate (S1P) to affect the migration, activation, differentiation, and polarization of macrophages. Recently, S1P and HIF-1α have received much attention, and various studies have been performed to investigate their roles in initiating and resolving inflammation via macrophages. It is hypothesized that the HIF-1α/S1P/S1P receptor axis is an important determinant of macrophage function under inflammatory conditions and during disease pathogenesis. Therefore, in this review, biological regulation of monocytes/macrophages in response to circulating HIF-1α is summarized, including signaling by S1P/S1P receptors, which have essential roles in wound healing

Macrophage Motility in Wound Healing Is Regulated by HIF-1 alpha via S1P Signaling

Accumulating evidence indicates that the molecular pathways mediating wound healing induce cell migration and localization of cytokines to sites of injury. Macrophages are immune cells that sense and actively respond to disturbances in tissue homeostasis by initiating, and subsequently resolving, inflammation. Hypoxic conditions generated at a wound site also strongly recruit macrophages and affect their function. Hypoxia inducible factor (HIF)-1 alpha is a transcription factor that contributes to both glycolysis and the induction of inflammatory genes, while also being critical for macrophage activation. For the latter, HIF-1 alpha regulates sphingosine 1-phosphate (S1P) to affect the migration, activation, differentiation, and polarization of macrophages. Recently, S1P and HIF-1 alpha have received much attention, and various studies have been performed to investigate their roles in initiating and resolving inflammation via macrophages. It is hypothesized that the HIF-1 alpha/S1P/S1P receptor axis is an important determinant of macrophage function under inflammatory conditions and during disease pathogenesis. Therefore, in this review, biological regulation of monocytes/macrophages in response to circulating HIF-1 alpha is summarized, including signaling by S1P/S1P receptors, which have essential roles in wound healing