Frequency of nodal metastasis in differentiated thyroid carcinoma

Background: Thyroid cancer is the most common endocrine malignancy. Moreover, within thyroid cancer, differentiated thyroid carcinoma (DTC) is the most common variety, with the incidence rising over the past decade. Often, most of the cases present with multicentric disease and presenting with lymph node metastases at the time of diagnosis. Nodal metastasis has prognostic importance, and it may guide surgeons regarding further management. Considering this scientific fact, the study was performed to see the frequency of lymph node metastasis in DTC among the patients admitted to a tertiary care hospital. Methods: This cross-sectional study was conducted in the department of otolaryngology and head-neck surgery, Sher-E-Bangla Medical College Hospital, for 9 months after the acceptance of the protocol. A total of 50 patients (in all age groups) who attended the relevant department due to thyroid malignancies were approached and interviewed. Thorough history taking, physical examination, and relevant investigation were done and recorded into separate case record forms. Informed written consent was taken from each subject. Following collection, data were coded and inputted into statistical software. Data analysis was done with SPSS 21 according to the objective of the study. Data were presented in the form of tables, and charts. Results: Among the 50 patients, the mean age of the patients was 47.86±15.69 SD (years) with minimum and maximum ages of 14 and 78 years respectively. The male-female ratio was 1:4 (20% male vs 80% female). Papillary carcinoma was the most frequent (88%) followed by follicular (10%). Nodal metastasis was most common in the papillary variety, about 54.55% of cases, whereas 20% were present in the follicular variety. The majority of the patients underwent total thyroidectomy with neck dissection (90%) and the remaining cases were managed by lobectomy (10%). Conclusions: This study concluded that nodal metastasis was present in 54.55% of cases of papillary carcinoma.

Assessing socio-economic patterns and trends of livelihoods of fisher’s community of the Old Brahmaputra River: A case study in Jamalpur, Bangladesh

The current study was assessed the socio-economic patterns and trends of livelihoods of the Old Brahmaputra River fisher's community in the Jamalpur district from July 2021 to June 2022. The livelihood status was analyzed regarding age distribution, family size and composition, religion, marital status, education levels, living standards, diseases, healthcare, electricity, sanitation, drinking water facilities, fishing, and employment status. Most fishermen (45.76%) were 35-50 years old, with 91.53% were Muslim. The average family size for a fisher was medium, with 5-7 members. 33.9% were illiterate, 37.29% could only sign, 20.34% finished primary school, and 8.47% completed SSC. Most fishermen had tin roofs 59.32%, straw roofs 22.03%, semi-paka houses 11.87%, and paka houses 6.78%. Approximately 45.76% of fishers relied on village doctors, 33.9% on the Upazila Health Complex, 8.47% on Kobiraj, and 11.87% on MBBS doctors. Approximately 93.22% of fishermen's homes were equipped with electricity. Fishing was the main activity for more than 57.63% of the fishers. Fishermen have suggested specific management strategies to halt the trend of decreasing fish catches and increasing fish production. These include restricting the use of harmful gear, prohibiting the catching of juvenile fish, creating fish sanctuaries, and releasing fish fry

Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA

<p>Abstract</p> <p>Background</p> <p>To obtain reliable quantitative real-time PCR data, normalization relative to stable housekeeping genes (HKGs) is required. However, in practice, expression levels of 'typical' housekeeping genes have been found to vary between tissues and under different experimental conditions. To date, validation studies of reference genes in pigs are relatively rare and have never been performed in porcine alveolar macrophages (AMs). In this study, expression stability of putative housekeeping genes were identified in the porcine AMs in response to the stimulation with two pathogen-associated molecular patterns (PAMPs) lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Three different algorithms (geNorm, Normfinder and BestKeeper) were applied to assess the stability of HKGs.</p> <p>Results</p> <p>The mRNA expression stability of nine commonly used reference genes (<it>B2M, BLM, GAPDH, HPRT1, PPIA, RPL4, SDHA, TBP </it>and <it>YWHAZ</it>) was determined by qRT-PCR in AMs that were stimulated by LPS and LTA <it>in vitro</it>. mRNA expression levels of all genes were found to be affected by the type of stimulation and duration of the stimulation (<it>P </it>< 0.0001). geNorm software revealed that <it>SDHA, B2M </it>and <it>RPL4 </it>showed a high expression stability in the irrespective to the stimulation group, while <it>SDHA, YWHAZ </it>and <it>RPL4 </it>showed high stability in non-stimulated control group. In all cases, <it>GAPDH </it>showed the least stability in geNorm. NormFinder revealed that <it>SDHA </it>was the most stable gene in all the groups. Moreover, geNorm software suggested that the geometric mean of the three most stable genes would be the suitable combination for accurate normalization of gene expression study.</p> <p>Conclusions</p> <p>There was discrepancy in the ranking order of reference genes obtained by different analysing algorithms. In conclusion, the geometric mean of the <it>SDHA, YWHAZ </it>and <it>RPL4 </it>seemed to be the most appropriate combination of HKGs for accurate normalization of gene expression data in porcine AMs without knowing the type of bacterial pathogenic status of the animals.</p

Volumetric Properties of Binary Mixtures of 2-Ethoxyethanol and 2-Butoxyethanol with 1,4-Dioxane

Densities, r, of 2-Ethoxyethanol (EGMEE) + 1,4-Dioxane (DXN) and  2-Butoxyethanol (EGMBE) + 1,4-Dioxane (DXN) systems have been measured in the whole range of composition at an interval of 5 K ranging from 303.15 to 323.15 K. Excess molar volumes, , partial molar volumes, , thermal expansivities,a, and excess thermal expansivities, aE, have been estimated from the experimental values of ρ. All of the derived properties have been fitted to appropriate polynomials. Values of   and aE were fitted to the Redlich-Kister polynomial equation and their variations with composition and temperature have been discussed in terms of molecular interaction in the mixtures. Values of  and aE were all positive and were attributed due to specific interactions

PANC Study (Pancreatitis: A National Cohort Study): national cohort study examining the first 30 days from presentation of acute pancreatitis in the UK

Abstract Background Acute pancreatitis is a common, yet complex, emergency surgical presentation. Multiple guidelines exist and management can vary significantly. The aim of this first UK, multicentre, prospective cohort study was to assess the variation in management of acute pancreatitis to guide resource planning and optimize treatment. Methods All patients aged greater than or equal to 18 years presenting with acute pancreatitis, as per the Atlanta criteria, from March to April 2021 were eligible for inclusion and followed up for 30 days. Anonymized data were uploaded to a secure electronic database in line with local governance approvals. Results A total of 113 hospitals contributed data on 2580 patients, with an equal sex distribution and a mean age of 57 years. The aetiology was gallstones in 50.6 per cent, with idiopathic the next most common (22.4 per cent). In addition to the 7.6 per cent with a diagnosis of chronic pancreatitis, 20.1 per cent of patients had a previous episode of acute pancreatitis. One in 20 patients were classed as having severe pancreatitis, as per the Atlanta criteria. The overall mortality rate was 2.3 per cent at 30 days, but rose to one in three in the severe group. Predictors of death included male sex, increased age, and frailty; previous acute pancreatitis and gallstones as aetiologies were protective. Smoking status and body mass index did not affect death. Conclusion Most patients presenting with acute pancreatitis have a mild, self-limiting disease. Rates of patients with idiopathic pancreatitis are high. Recurrent attacks of pancreatitis are common, but are likely to have reduced risk of death on subsequent admissions. </jats:sec

Hematological reference values for healthy fat-tailed sheep (Dhumba) in Bangladesh

Objective: There is scarce literature regarding hematology profile of fat-tailed sheep (Dhumba). The study was conducted to determine reference intervals for their hematology profile in the context of Bangladesh. Materials and methods: Blood samples were collected from 32 healthy fat-tailed sheep from Dhaka, Bangladesh, during SeptemberOctober, 2015. Hemoglobin (Hb), packed cell volume (PCV), erythrocyte sedimentation rate (ESR), total leukocyte count (TLC), total erythrocyte count (TEC), lymphocyte, monocyte, eosinophil, basophil, and neutrophil counts were measured. Results: The levels showed a wide range and variation based on age and sex. Adult sheep had significantly (p = 0.01) higher Hb level than that of juvenile ( [J Adv Vet Anim Res 2018; 5(4.000): 481-484

Isolation, identification and antimicrobial resistance profile of Staphylococcus aureus in Cockroaches (Periplaneta Americana)

Objective: The study was conducted to determine the prevalence of Staphylococcus aureus in cockroaches (Periplaneta americana), and to assess the antimicrobial resistance profiles of the isolated bacteria. Materials and methods: A total of 150 cockroaches (P. americana) were randomly captured from three households and four restaurants in Chittagong City Corporation, Bangladesh during July to December 2014. The cockroaches were transported to the bacteriology laboratory at the Poultry Research and Training Centre (PRTC), Chittagong Veterinary and Animal Sciences University. The isolation and identification of Staphylococcus spp. from the external surface wash and gut homogenates by pooling cockroaches were done by following conventional bacteriological examinations followed by biochemical characterization. The antibiotic susceptibility profiles of the isolates were determined using disc diffusion method. Results: In this study, the overall prevalence of S. aureus was 38% (n=57/150). Higher prevalence of Staphylococcus spp. was observed among the cockroaches from restaurant (49.3%; n=37/75) as compared to those of households (26.7%; n=20/75) having a significant difference (P<0.05). Highest level of resistance by the Staphylococcus spp. was found to Penicillin (68%) followed by Erythromycin (60%), Oxacillin (46%) and Clindamycin (31%). On the other hand, the Staphylococci isolates were highly sensitive to Cephalothin (84%) and Kanamycin (65%). Conclusion: The rational use of antibiotics needs to be adopted in both human and animal medicine practices to prevent the emergence of drug resistant Staphylococcus spp. [J Adv Vet Anim Res 2016; 3(3.000): 221-228

Trappc9 Deficiency Impairs the Plasticity of Stem Cells

Genetic mutations of trappc9 cause intellectual disability with the atrophy of brain structures and variable obesity by poorly understood mechanisms. Trappc9-deficient mice develop phenotypes resembling pathological changes in humans and appear overweight shortly after weaning, and thus are useful for studying the pathogenesis of obesity. Here, we investigated the effects of trappc9 deficiency on the proliferation and differentiation capacity of adipose-derived stem cells (ASCs). We isolated ASCs from mice before overweight was developed and found that trappc9-null ASCs exhibited signs of premature senescence and cell death. While the lineage commitment was retained, trappc9-null ASCs preferred adipogenic differentiation. We observed a profound accumulation of lipid droplets in adipogenic cells derived from trappc9-deficient ASCs and marked differences in the distribution patterns and levels of calcium deposited in osteoblasts obtained from trappc9-null ASCs. Biochemical studies revealed that trappc9 deficiency resulted in an upregulated expression of rab1, rab11, and rab18, and agitated autophagy in ASCs. Moreover, we found that the content of neural stem cells in both the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus vastly declined in trappc9-null mice. Collectively, our results suggest that obesity, as well as brain structure hypoplasia induced by the deficiency of trappc9, involves an impairment in the plasticity of stem cells