Interactive Assembly and Animation of 3D Digital Garments

We present a novel real-time tool for sewing together 2D patterns, enabling quick assembly of visually plausible, interactively animated garments for virtual characters. The process is assisted by ad-hoc visual hints and allows designers to import 2D patterns from any CAD-tool, connect them using seams around a 3D character with any body type, and assess the overall quality during the character animation. The cloth is numerically simulated including robust modeling of contact of the cloth with itself and with the character\u27s body. Overall, our tool allows for fast prototyping of virtual garments, achieving immediate feedback on their behaviour and visual quality on an animated character, in effect speeding up the content production pipeline for visual effects applications involving clothed characters

Altered expression of topoisomerase IIα contributes to cross-resistant to etoposide K562/MX2 cell line by aberrant methylation

KRN 8602 (MX2) is a novel morpholino anthracycline derivative having the chemical structure 3′-deamino-3′-morpholino-13-deoxo-10-hydroxycarminomycin hydrochloride. To investigate the mechanisms of resistance to MX2, we established an MX2-resistant phenotype (K562/MX2) of the human myelogeneous leukaemia cell line (K562/P), by continuously exposing a suspension culture to increasing concentrations of MX2. K562/MX2 cells were more resistant to MX2 than the parent cells, and also showed cross-resistance to etoposide and doxorubicin. Topoisomerase (Topo) IIα protein levels in K562/MX2 cells were lower of those in K562/P cells on immunoblot analysis and decreased expression of Topo IIα mRNA was seen in K562/MX2 cells. Topoisomerase II catalytic activity was also reduced in the nuclear extracts from K562/MX2 cells when compared with K562/P cells. Aberrant methylated CpG of Topo IIα gene was observed in K562/MX2 cells when compared with the parent line on methylation-specific restriction enzyme analysis. To overcome the drug resistance to MX2 and etoposide, we investigated treatment with 5-Aza-2′-deoxycytidine (5AZ), which is a demethylating agent, in K562/MX2 cells. 5-Aza-2′-deoxycytidine treatment increased Topo IIα mRNA expression in K562/MX2 cells, but not in K562/P cells, and increased the cytotoxicity of MX2 and etoposide. Methylated CpG was decreased in K562/MX2 cells after 5AZ treatment. We concluded that the mechanism of drug resistance to MX2 and etoposide in K562/MX2 cells might be the combination of decreased expression of Topo IIα gene and increased methylation, and that 5AZ could prove to be a novel treatment for etoposide-resistant cell lines, such as K562/MX2

Crosstalks between Myo-Inositol Metabolism, Programmed Cell Death and Basal Immunity in Arabidopsis

BACKGROUND: Although it is a crucial cellular process required for both normal development and to face stress conditions, the control of programmed cell death in plants is not fully understood. We previously reported the isolation of ATXR5 and ATXR6, two PCNA-binding proteins that could be involved in the regulation of cell cycle or cell death. A yeast two-hybrid screen using ATXR5 as bait captured AtIPS1, an enzyme which catalyses the committed step of myo-inositol (MI) biosynthesis. atips1 mutants form spontaneous lesions on leaves, raising the possibility that MI metabolism may play a role in the control of PCD in plants. In this work, we have characterised atips1 mutants to gain insight regarding the role of MI in PCD regulation. METHODOLOGY/PRINCIPAL FINDINGS: - lesion formation in atips1 mutants depends of light intensity, is due to PCD as evidenced by TUNEL labelling of nuclei, and is regulated by phytohormones such as salicylic acid - MI and galactinol are the only metabolites whose accumulation is significantly reduced in the mutant, and supplementation of the mutant with these compounds is sufficient to prevent PCD - the transcriptome profile of the mutant is extremely similar to that of lesion mimic mutants such as cpr5, or wild-type plants infected with pathogens. CONCLUSION/SIGNIFICANCE: Taken together, our results provide strong evidence for the role of MI or MI derivatives in the regulation of PCD. Interestingly, there are three isoforms of IPS in Arabidopsis, but AtIPS1 is the only one harbouring a nuclear localisation sequence, suggesting that nuclear pools of MI may play a specific role in PCD regulation and opening new research prospects regarding the role of MI in the prevention of tumorigenesis. Nevertheless, the significance of the interaction between AtIPS1 and ATXR5 remains to be established

光合成生物におけるアスコルビン酸ペルオキシダーゼの分子特性の解明

[CONTENTS] CHAPTER I. Introduction, CHAPTER II. Molecular Characterization of Euglena Ascorbate Peroxidase Using Monoclonal Antibody, CHAPTER III. Effect of Iron on the Expression of Ascorbate Peroxidase in Euglena, CHAPTER IV. Effects of Illumination on the Induction of Ascorbate Peroxidase and Enzymes Related to Ascorbate-Glutathione Cycle in Euglena gracilis Z, CHAPTER V. Hydrogen Peroxide Generation in Organelles of Euglena grncilis Z, CHAPTER VI. Purification and Characterization of Cytosolic Ascorbate Peroxidase from Komatsuna (Brassica rapa), CHAPTER VII. Cloning and Expression of cDNA Encoding a New Type of Ascorbate Peroxidase from Spinach, CHAPTER VIII. Cloning and Sequence Analysis of a cDNA Encoding Chloroplastic Ascorbate Peroxidase from Spinach, CHAPTER IX. Conclusion, REFERENCES, PUBLICATIONS学位の種類：農学　　学位授与年月日：1996/3/25　　指導：重岡, 成 教授 　　報告番号：甲第342号　　学内授与番号：農第１１号 　NDL書誌ID：00000029657

Reinforcement Learning for a New Piano Mover

We attempt to achieve corporative behavior of autonomous decentralized agents constructed via Q-Learning, which is a type of reinforcement learning. As such, in the present paper, we examine the piano mover's problem. We propose a multi-agent architecture that has a training agent, learning agents and intermediate agent. Learning agents are heterogeneous and can communicate with each other. The movement of an object with three kinds of agent depends on the composition of the actions of the learning agents. By learning its own shape through the learning agents, avoidance of obstacles by the object is expected. We simulate the proposed method in a two-dimensional continuous world. Results obtained in the present investigation reveal the effectiveness of the proposed method