42 research outputs found
ATP-Stimulated, DNA-Mediated Redox Signaling by XPD, a DNA Repair and Transcription Helicase
Using DNA-modified electrodes, we show DNA-mediated signaling by XPD, a helicase that contains a [4Fe-4S] cluster and is critical for nucleotide excision repair and transcription. The DNA-mediated redox signal resembles that of base excision repair proteins, with a DNA-bound redox potential of ~80 mV versus NHE. Significantly, this signal increases with ATP hydrolysis. Moreover, the redox signal is substrate-dependent, reports on the DNA conformational changes associated with enzymatic function, and may reflect a general biological role for DNA charge transport
Spectral Energy Distributions of Local Luminous And Ultraluminous Infrared Galaxies
Luminous and ultraluminous infrared galaxies ((U)LIRGs) are the most extreme
star forming galaxies in the universe. The local (U)LIRGs provide a unique
opportunity to study their multi-wavelength properties in detail for comparison
to their more numerous counterparts at high redshifts. We present common large
aperture photometry at radio through X-ray wavelengths, and spectral energy
distributions (SEDs) for a sample of 53 nearby LIRGs and 11 ULIRGs spanning log
(LIR/Lsun) = 11.14-12.57 from the flux-limited Great Observatories All-sky LIRG
Survey (GOALS). The SEDs for all objects are similar in that they show a broad,
thermal stellar peak and a dominant FIR thermal dust peak, where nuLnu(60um) /
nuLnu(V) increases from ~2-30 with increasing LIR. When normalized at
IRAS-60um, the largest range in the luminosity ratio,
R(lambda)=log[nuLnu(lambda)/nuLnu(60um)] observed over the full sample is seen
in the Hard X-rays (HX=2-10 keV). A small range is found in the Radio (1.4GHz),
where the mean ratio is largest. Total infrared luminosities, LIR(8-1000um),
dust temperatures, and dust masses were computed from fitting thermal dust
emission modified blackbodies to the mid-infrared (MIR) through submillimeter
SEDs. The new results reflect an overall ~0.02 dex lower luminosity than the
original IRAS values. Total stellar masses were computed by fitting stellar
population synthesis models to the observed near-infrared (NIR) through
ultraviolet (UV) SEDs. Mean stellar masses are found to be log(M/Msun) =
10.79+/-0.40. Star formation rates have been determined from the infrared
(SFR_IR~45Msun/yr) and from the monochromatic UV luminosities
(SFR_UV~1.3Msun/yr), respectively. Multiwavelength AGN indicators have be used
to select putative AGN: about 60% of the ULIRGs would have been classified as
an AGN by at least one of the selection criteria.Comment: 39 pages, including 12 figures and 11 tables; accepted for
publication in ApJ
Tracing PAHs and Warm Dust Emission in the Seyfert Galaxy NGC 1068
We present a study of the nearby Seyfert galaxy NGC 1068 using mid- and far-
infrared data acquired with the IRAC, IRS, and MIPS instruments aboard the
Spitzer Space Telescope. The images show extensive 8 um and 24 um emission
coinciding with star formation in the inner spiral approximately 15" (1 kpc)
from the nucleus, and a bright complex of star formation 47" (3 kpc) SW of the
nucleus. The brightest 8 um PAH emission regions coincide remarkably well with
knots observed in an Halpha image. Strong PAH features at 6.2, 7.7, 8.6, and
11.3 um are detected in IRS spectra measured at numerous locations inside,
within, and outside the inner spiral. The IRAC colors and IRS spectra of these
regions rule out dust heated by the AGN as the primary emission source; the
SEDs are dominated by starlight and PAH emission. The equivalent widths and
flux ratios of the PAH features in the inner spiral are generally consistent
with conditions in a typical spiral galaxy ISM. Interior to the inner spiral,
the influence of the AGN on the ISM is evident via PAH flux ratios indicative
of a higher ionization parameter and a significantly smaller mean equivalent
width than observed in the inner spiral. The brightest 8 and 24 um emission
peaks in the disk of the galaxy, even at distances beyond the inner spiral, are
located within the ionization cones traced by [O III]/Hbeta, and they are also
remarkably well aligned with the axis of the radio jets. Although it is
possible that radiation from the AGN may directly enhance PAH excitation or
trigger the formation of OB stars that subsequently excite PAH emission at
these locations in the inner spiral, the orientation of collimated radiation
from the AGN and star formation knots in the inner spiral could be
coincidental. (abridged)Comment: 20 pages, 11 figures; AJ, accepted; full resolution version available
at http://spider.ipac.caltech.edu/staff/jhhowell/astro/howelln1068.pd
Increased Secreted Amyloid Precursor Protein-α (sAPPα) in Severe Autism: Proposal of a Specific, Anabolic Pathway and Putative Biomarker
Autism is a neurodevelopmental disorder characterized by deficits in verbal communication, social interactions, and the presence of repetitive, stereotyped and compulsive behaviors. Excessive early brain growth is found commonly in some patients and may contribute to disease phenotype. Reports of increased levels of brain-derived neurotrophic factor (BDNF) and other neurotrophic-like factors in autistic neonates suggest that enhanced anabolic activity in CNS mediates this overgrowth effect. We have shown previously that in a subset of patients with severe autism and aggression, plasma levels of the secreted amyloid-β (Aβ) precursor protein-alpha form (sAPPα) were significantly elevated relative to controls and patients with mild-to-moderate autism. Here we further tested the hypothesis that levels of sAPPα and sAPPβ (proteolytic cleavage products of APP by α- and β-secretase, respectively) are deranged in autism and may contribute to an anabolic environment leading to brain overgrowth. We measured plasma levels of sAPPα, sAPPβ, Aβ peptides and BDNF by corresponding ELISA in a well characterized set of subjects. We included for analysis 18 control, 6 mild-to-moderate, and 15 severely autistic patient plasma samples. We have observed that sAPPα levels are increased and BDNF levels decreased in the plasma of patients with severe autism as compared to controls. Further, we show that Aβ1-40, Aβ1-42, and sAPPβ levels are significantly decreased in the plasma of patients with severe autism. These findings do not extend to patients with mild-to-moderate autism, providing a biochemical correlate of phenotypic severity. Taken together, this study provides evidence that sAPPα levels are generally elevated in severe autism and suggests that these patients may have aberrant non-amyloidogenic processing of APP
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Addressing ecological effects of radiation on populations and ecosystems to improve protection of the environment against radiation: Agreed statements from a Consensus Symposium
This paper reports the output of a consensus symposium organized by the International Union of Radioecology in November 2015. The symposium gathered an academically diverse group of 30 scientists to consider the still debated ecological impact of radiation on populations and ecosystems. Stimulated by the Chernobyl and Fukushima disasters' accidental contamination of the environment, there is increasing interest in developing environmental radiation protection frameworks. Scientific research conducted in a variety of laboratory and field settings has improved our knowledge of the effects of ionizing radiation on the environment. However, the results from such studies sometimes appear contradictory and there is disagreement about the implications for risk assessment. The Symposium discussions therefore focused on issues that might lead to different interpretations of the results, such as laboratory versus field approaches, organism versus population and ecosystemic inference strategies, dose estimation approaches and their significance under chronic exposure conditions. The participating scientists, from across the spectrum of disciplines and research areas, extending also beyond the traditional radioecology community, successfully developed a constructive spirit directed at understanding discrepancies. From the discussions, the group has derived seven consensus statements related to environmental protection against radiation, which are supplemented with some recommendations. Each of these statements is contextualized and discussed in view of contributing to the orientation and integration of future research, the results of which should yield better consensus on the ecological impact of radiation and consolidate suitable approaches for efficient radiological protection of the environment.Keywords: Consensus development, Environmental protection, Populations, Radiation effects, Ecosystems, Ecological risk assessmen
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Chapter One MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes
Recombinant expression of large, multiprotein complexes is essential and often rate limiting for determining structural, biophysical, and biochemical properties of DNA repair, replication, transcription, and other key cellular processes. Baculovirus-infected insect cell expression systems are especially well suited for producing large, human proteins recombinantly, and multigene baculovirus systems have facilitated studies of multiprotein complexes. In this chapter, we describe a multigene baculovirus system called MacroBac that uses a Biobricks-type assembly method based on restriction and ligation (Series 11) or ligation-independent cloning (Series 438). MacroBac cloning and assembly is efficient and equally well suited for either single subcloning reactions or high-throughput cloning using 96-well plates and liquid handling robotics. MacroBac vectors are polypromoter with each gene flanked by a strong polyhedrin promoter and an SV40 poly(A) termination signal that minimize gene order expression level effects seen in many polycistronic assemblies. Large assemblies are robustly achievable, and we have successfully assembled as many as 10 genes into a single MacroBac vector. Importantly, we have observed significant increases in expression levels and quality of large, multiprotein complexes using a single, multigene, polypromoter virus rather than coinfection with multiple, single-gene viruses. Given the importance of characterizing functional complexes, we believe that MacroBac provides a critical enabling technology that may change the way that structural, biophysical, and biochemical research is done
Addressing ecological effects of radiation on populations and ecosystems to improve protection of the environment against radiation : agreed statements from a Consensus Symposium
This paper reports the output of a consensus symposium organized by the International Union of
Radioecology in November 2015. The symposium gathered an academically diverse group of 30 scientists
to consider the still debated ecological impact of radiation on populations and ecosystems. Stimulated by
the Chernobyl and Fukushima disasters' accidental contamination of the environment, there is increasing
interest in developing environmental radiation protection frameworks. Scientific research conducted in a
variety of laboratory and field settings has improved our knowledge of the effects of ionizing radiation on
the environment. However, the results from such studies sometimes appear contradictory and there is
disagreement about the implications for risk assessment. The Symposium discussions therefore focused
on issues that might lead to different interpretations of the results, such as laboratory versus field approaches,
organism versus population and ecosystemic inference strategies, dose estimation approaches
and their significance under chronic exposure conditions. The participating scientists, from across the
spectrum of disciplines and research areas, extending also beyond the traditional radioecology community,
successfully developed a constructive spirit directed at understanding discrepancies. From the
discussions, the group has derived seven consensus statements related to environmental protection
against radiation, which are supplemented with some recommendations. Each of these statements is
contextualized and discussed in view of contributing to the orientation and integration of future
research, the results of which should yield better consensus on the ecological impact of radiation and
consolidate suitable approaches for efficient radiological protection of the environment.peerReviewe
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BrechignacAddressingEcologicalEffectsAppendix.pdf
This paper reports the output of a consensus symposium organized by the International Union of Radioecology in November 2015. The symposium gathered an academically diverse group of 30 scientists to consider the still debated ecological impact of radiation on populations and ecosystems. Stimulated by the Chernobyl and Fukushima disasters' accidental contamination of the environment, there is increasing interest in developing environmental radiation protection frameworks. Scientific research conducted in a variety of laboratory and field settings has improved our knowledge of the effects of ionizing radiation on the environment. However, the results from such studies sometimes appear contradictory and there is disagreement about the implications for risk assessment. The Symposium discussions therefore focused on issues that might lead to different interpretations of the results, such as laboratory versus field approaches, organism versus population and ecosystemic inference strategies, dose estimation approaches and their significance under chronic exposure conditions. The participating scientists, from across the spectrum of disciplines and research areas, extending also beyond the traditional radioecology community, successfully developed a constructive spirit directed at understanding discrepancies. From the discussions, the group has derived seven consensus statements related to environmental protection against radiation, which are supplemented with some recommendations. Each of these statements is contextualized and discussed in view of contributing to the orientation and integration of future research, the results of which should yield better consensus on the ecological impact of radiation and consolidate suitable approaches for efficient radiological protection of the environment.Keywords: Environmental protection, Populations, Radiation effects, Consensus development, Ecosystems, Ecological risk assessmen
Recommended from our members
BrechignacAddressingEcologicalEffects.pdf
This paper reports the output of a consensus symposium organized by the International Union of Radioecology in November 2015. The symposium gathered an academically diverse group of 30 scientists to consider the still debated ecological impact of radiation on populations and ecosystems. Stimulated by the Chernobyl and Fukushima disasters' accidental contamination of the environment, there is increasing interest in developing environmental radiation protection frameworks. Scientific research conducted in a variety of laboratory and field settings has improved our knowledge of the effects of ionizing radiation on the environment. However, the results from such studies sometimes appear contradictory and there is disagreement about the implications for risk assessment. The Symposium discussions therefore focused on issues that might lead to different interpretations of the results, such as laboratory versus field approaches, organism versus population and ecosystemic inference strategies, dose estimation approaches and their significance under chronic exposure conditions. The participating scientists, from across the spectrum of disciplines and research areas, extending also beyond the traditional radioecology community, successfully developed a constructive spirit directed at understanding discrepancies. From the discussions, the group has derived seven consensus statements related to environmental protection against radiation, which are supplemented with some recommendations. Each of these statements is contextualized and discussed in view of contributing to the orientation and integration of future research, the results of which should yield better consensus on the ecological impact of radiation and consolidate suitable approaches for efficient radiological protection of the environment.Keywords: Ecosystems, Populations, Consensus development, Environmental protection, Ecological risk assessment, Radiation effect