Terrorism as a Media Specific Event: Performative Frames of Uri and Pulwama Reportage in Indian News Media

The Uri attack in 2016 and the Pulwama attack in 2019 by terrorist in Jammu and Kashmir have brought to the center-stage the Kashmir conflict as a core issue between the two South Asian neighbors, India and Pakistan. This research analyzes the mediatized indulgence of terror in reporting of these two incidents in television and print media. The politics of representation in this analysis entails that these frames do not just involve reportage but an act of performance, how events are choreographed and predated on emotions and sentiments as cultural practices mobilizing its effective economy. This paper through the prism of media-industrial-terror complex draws upon the theory of critical events and focuses on how particular events – Uri and Pulwama activate and mobilize a discursive master narrative of Pakistan inspired/directed terrorism by ascribing a particular meaning to the war on terror. The semiotics of media reporting of Uri and Pulwama is analyzed through the tripartite motive quotient of gham (remorse), gussa (anger), and garv (pride)

Terrorism as a Media Specific Event: Performative Frames of Uri and Pulwama Reportage in Indian News Media

The Uri attack in 2016 and the Pulwama attack in 2019 by terrorist in Jammu and Kashmir have brought to the center-stage the Kashmir conflict as a core issue between the two South Asian neighbors, India and Pakistan. This research analyzes the mediatized indulgence of terror in reporting of these two incidents in television and print media. The politics of representation in this analysis entails that these frames do not just involve reportage but an act of performance, how events are choreographed and predated on emotions and sentiments as cultural practices mobilizing its effective economy. This paper through the prism of media-industrial-terror complex draws upon the theory of critical events and focuses on how particular events – Uri and Pulwama activate and mobilize a discursive master narrative of Pakistan inspired/directed terrorism by ascribing a particular meaning to the war on terror. The semiotics of media reporting of Uri and Pulwama is analyzed through the tripartite motive quotient of gham (remorse), gussa (anger), and garv (pride)

Computed tomography diagnosis of truncus arteriosus type IV: a case report

Truncus arteriosus (TA) is an uncommon congenital cardiac anomaly of which type IV is considered a rare variant. Recognition is crucial for proper treatment planning. The prognosis without treatment is poor. Echocardiography alone may not be useful in evaluation. Computed tomography (CT) finding is complicated. We report an 18 months child with ventricular septal defect (VSD), diagnosed on echocardiography, and further review by CT showed VSD with descending thoracic aorta giving rise to the pulmonary arteries suggestive of pseudo truncus (Collet and Edwards Truncus arteriosus Type IV) and right sided aortic arch with mirror image branching. Keywords: echocardiography, Collet and Edwards, computed tomography (CT), truncus arteriosus, ventricular septal defect (VSD

Isolation and identification of Concanavalin A binding glycoproteins from human seminal plasma: A step towards identification of male infertility marker proteins

Abstract. Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In this study, we have isolated Concanavalin-A binding glycoproteins from human seminal plasma and subsequently identified them by MALDI-TOF/MS analysis. The major proteins, as identified in this study, are Aminopeptidase N, lactoferrin, prostatic acid phosphatase, zinc-alpha-2-glycoprotein, prostate specific antigen, progestagen-associated endometrial protein, Izumo sperm-egg fusion protein and prolactin inducible protein. This paper also reports preliminary studies to identify altered expression of these proteins in oligospermia and azoospermia in comparison to normospermia. In oligospermia, five proteins were found to be downregulated while in azoospermia, four proteins were downregulated and two proteins were upregulated. Thus, this study is of immense biomedical interest towards identification of potential male infertility marker proteins in seminal plasma

Achieving Surgical, Obstetric, Trauma, and Anesthesia (SOTA) care for all in South Asia

South Asia is a demographically crucial, economically aspiring, and socio-culturally diverse region in the world. The region contributes to a large burden of surgically-treatable disease conditions. A large number of people in South Asia cannot access safe and affordable surgical, obstetric, trauma, and anesthesia (SOTA) care when in need. Yet, attention to the region in Global Surgery and Global Health is limited. Here, we assess the status of SOTA care in South Asia. We summarize the evidence on SOTA care indicators and planning. Region-wide, as well as country-specific challenges are highlighted. We also discuss potential directions—initiatives and innovations—toward addressing these challenges. Local partnerships, sustained research and advocacy efforts, and politics can be aligned with evidence-based policymaking and health planning to achieve equitable SOTA care access in the South Asian region under the South Asian Association for Regional Cooperation (SAARC)

Virtual Screening, Identification and In Vitro Testing of Novel Inhibitors of O-Acetyl-L-Serine Sulfhydrylase of

The explosive epidemicity of amoebiasis caused by the facultative gastrointestinal protozoan parasite Entamoeba histolytica is a major public health problem in developing countries. Multidrug resistance and side effects of various available antiamoebic drugs necessitate the design of novel antiamobeic agents. The cysteine biosynthetic pathway is the critical target for drug design due to its significance in the growth, survival and other cellular activities of E. histolytica. Here, we have screened 0.15 million natural compounds from the ZINC database against the active site of the EhOASS enzyme (PDB ID. 3BM5, 2PQM), whose structure we previously determined to 2.4 A ˚ and 1.86 A ˚ resolution. For this purpose, the incremental construction algorithm of GLIDE and the genetic algorithm of GOLD were used. We analyzed docking results for top ranking compounds using a consensus scoring function of X-Score to calculate the binding affinity and using ligplot to measure protein-ligand interactions. Fifteen compounds that possess good inhibitory activity against EhOASS active site were identified that may act as potential high affinity inhibitors. In vitro screening of a few commercially available compounds established their biological activity. The first ranked compound ZINC08931589 had a binding affinity of,8.05 mM and inhibited about 73 % activity at 0.1 mM concentration, indicating good correlation between in silico prediction and in vitro inhibition studies. This compound is thus a good starting point for further development of stron

Top ranking ligands (Natural compounds from Zinc database) after virtual screening against EhOASS using GLIDE and GOLD docking programs.

<p>Top ranking ligands (Natural compounds from Zinc database) after virtual screening against EhOASS using GLIDE and GOLD docking programs.</p

The plot between ΔF/F vs. inhibitor concentration follows a typical hyperbolic similar to Michaelis-Menton curve, the plot between 1/ΔF/F vs. inhibitor concentration (the reciprocal plot) follows that of a Lineweaver-Burk plot, the Kd values of inhibitor A) ZINC08931589 and B) ZINC03984585 were determined from these plots.

<p>The plot between ΔF/F vs. inhibitor concentration follows a typical hyperbolic similar to Michaelis-Menton curve, the plot between 1/ΔF/F vs. inhibitor concentration (the reciprocal plot) follows that of a Lineweaver-Burk plot, the Kd values of inhibitor A) ZINC08931589 and B) ZINC03984585 were determined from these plots.</p

Virtual Screening, Identification and In Vitro Testing of Novel Inhibitors of O-Acetyl-L-Serine Sulfhydrylase of <em>Entamoeba histolytica</em>

<div><p>The explosive epidemicity of amoebiasis caused by the facultative gastrointestinal protozoan parasite <em>Entamoeba histolytica</em> is a major public health problem in developing countries. Multidrug resistance and side effects of various available antiamoebic drugs necessitate the design of novel antiamobeic agents. The cysteine biosynthetic pathway is the critical target for drug design due to its significance in the growth, survival and other cellular activities of <em>E. histolytica</em>. Here, we have screened 0.15 million natural compounds from the ZINC database against the active site of the EhOASS enzyme (PDB ID. 3BM5, 2PQM), whose structure we previously determined to 2.4 Å and 1.86 Å resolution. For this purpose, the incremental construction algorithm of GLIDE and the genetic algorithm of GOLD were used. We analyzed docking results for top ranking compounds using a consensus scoring function of X-Score to calculate the binding affinity and using ligplot to measure protein-ligand interactions. Fifteen compounds that possess good inhibitory activity against EhOASS active site were identified that may act as potential high affinity inhibitors. <em>In vitro</em> screening of a few commercially available compounds established their biological activity. The first ranked compound ZINC08931589 had a binding affinity of ∼8.05 µM and inhibited about 73% activity at 0.1 mM concentration, indicating good correlation between <em>in silico</em> prediction and <em>in vitro</em> inhibition studies. This compound is thus a good starting point for further development of strong inhibitors.</p> </div

Inhibition studies of EhOASS.

<p>The concentration of the inhibitor was kept at 100 µM and the readings were taken under two substrate concentrations; 0.5 mM (Km of the substrate) and 100 µM (for competitive inhibition, inhibitor and substrate were used same concentration).</p