Molybdenum sources and transport in the Alcator C-Mod tokamak

Additional file 6. Luciferase reporter assay results with or without L1H1-7OTD for G4 DNA sequences cloned in pGL4.23 vector

Mix Cropping Trial of Determinate and Indeterminate Soybean Lines in Kawatabi Field Science Center

Poster Session

Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors

We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals

NOVEL BIOSENSOR SYSTEMS FOR CLINICAL AND FOOD ANALYSIS USING MICRO DEVICES

The amorphous silicon ion sensitive field effect transistor (a-ISFET) made by radio frequency plasma discharge was used as a pH-sensitive device. The size of the channel of the a-ISFET is 10 um long and 500 um wide. The pH sensitive layer was silicon oxide evaporated over the amorphous silicon nitride layer. The pH sensitivity was about 46 mV/pH at 18°C in the range pH 5-10. The response times of this device to pH change are very rapid, being less than 30 sec to reach a steady state value. The fish freshness sensor was constructed by using an immobilized xantine oxidase membrane and a-ISFET. Hypoxantine was detected in the range 0.02-0.1mM. Micro-biosensors for glucose and glutamate were constructed by using silicon fabrication technology. A linear relationship was observed between the glucose concentration in the range from 0.1 to 10 mg aı-!, and the decrease in current. Glutamate was determined in the range from 5 to 50 mM. The microoxygen electrode consists of two gold electrodes and agarose gel containing electrolyte covered with a gas permeable membrane. A negative photoresist was used as the gas permeable membrane which was directly cast onto the gel and submitted to photochemical reaction. A 90% response time of the micro-oxygen electrode took approximately 3 minutes. A linear relationship was obtained between the response of the micro-oxygen electrode and that of a conventional Galvanic oxygen electrode