Reconstructing temperature at Egelsee, Switzerland, using North American and Swedish chironomid transfer functions: potential and pitfalls

The temperature reconstruction obtained from chironomids preserved in the sediment of Egelsee, Switzerland, was partially flawed by the low percentages of fossil taxa represented in the Swiss calibration set (Larocque-Tobler et al. 2009a). Transfer functions (TFs) from other regions, which allow a good representation of the fossil taxa (>80%), could be applied to the fossil assemblages of Egelsee. First, the validity of using two (a Swedish and a North American (NA)) TFs was tested by comparing the chironomid-inferred temperatures with instrumental data. Since good relationships (r Pearson=0.71 and 0.61, p=0.001 for the NA and Swedish TFs, respectively) were obtained, these two models were used to reconstruct the Late Glacial and early Holocene periods at Egelsee. Reconstructions using both models showed clear cold periods during the Younger Dryas and the so-called 8,200 calibrated years BP event. However, the amplitude of changes during these periods was higher when the NA transfer function was used, probably due to the fact that 37% of the taxa in the core had temperature optima colder in the NA than in the Swedish and Swiss models. The results indicate that TFs from other regions can be applied when they are based on samples with good modern analogues, however, caution should be taken when the amplitude of temperature changes is considere

The use of cotton blue stain to improve the efficiency of picking and identifying chironomid head capsules

Cotton blue was added to sediment samples at least 2h before chironomid head capsules were picked under a binocular microscope and mounted on slides for identification. The use of stain greatly increased the visibility of chironomid head capsules during picking and enhanced the contrast of various parts of the head capsules (pores, ventromental plates, striations on ventromental plates), which could aid identification. In the seven samples studied, there was no significant difference between the percentages of taxa found in stained and unstained samples. The number of taxa were also similar in stained and unstained samples. This method allowed samples to be picked faste

Effect of removing small (<150μm) chironomids on inferring temperature in cold lakes

Sieving samples for chironomid analysis with a 150μm mesh was shown to greatly reduce sample preparation time, and use of only larger specimens did not affect chironomid-inferred salinities in African lakes. Here, we tested if this method is suitable for temperature reconstruction in colder lakes at higher latitudes. Removal of specimens 150μm) than those obtained with the full model (all specimens >100μm). General patterns of temperature change were also altered. For Lake 7 on Southampton Island, Canada, a cooling trend was reconstructed with the full Canadian model while the modified Canadian model yielded a warming trend. When only specimens >150μm were used, two to three times more wet sediment was needed to obtain a sufficient number of head capsules. These results indicate that, in cold lakes (mean July/August air temperature ≤11°C), large proportions of head capsules are <150 μm, and sieving the samples in a 150μm mesh leads to altered temperature reconstruction

Late Glacial and Holocene temperature changes at Egelsee, Switzerland, reconstructed using subfossil chironomids

A temperature reconstruction using chironomids was attempted at Egelsee, Switzerland, a site where pollen and macrofossil records showed a correspondence between vegetation and climatic changes inferred by other proxies in Europe. The general pattern of temperature changes inferred from chironomids during the Late Glacial [i.e. cold temperatures between ca. 16,500 and 14,800cal BP, close to present-day temperature between 15,000 and 13,000cal BP and colder temperatures during the Younger Dryas (YD)], and the major temperature changes of the Holocene (i.e. the Younger Dryas-Holocene transition and the Late Holocene cooling trend) at Egelsee, were mirrored in other European climate reconstructions using various proxies. However, the amplitude of temperature changes during the YD was smaller than reconstructed by other proxies at various sites, and the 8,200years BP event was not apparent. These differences between records were probably due to the dominance of Corynocera ambigua, with percentages reaching 60% in parts of the Egelsee sequence. This taxon was not present in any of the 103 lakes used for the transfer function and its absence may have yielded less accurate inferences. Its presence in samples only associated with cold inferences at Egelsee suggests that this taxon is a cold indicator. However, it was also found in warm Danish lakes and the factors that determine the presence of C. ambigua remain unexplained. Most samples had a poor fit to temperature and instead, dissolved organic carbon seemed to be a factor influencing the chironomid assemblages during the Holocene. These results illustrate the need to better understand the ecology of chironomids and to disentangle the various factors that affect chironomid communities through time. Ultimately, such information will lead to more accurate temperature reconstruction

Comparison between chironomid-inferred July temperatures and meteorological data AD 1850-2001 from varved Lake Silvaplana, Switzerland

Inferred temperatures from chironomids preserved in the varved sediment of Lake Silvaplana in the Eastern Swiss Alps were compared with instrumental data obtained from a meteorological station in Sils-Maria, on the shore of Lake Silvaplana, for the time interval 1850-2001. At near-annual resolution, the general patterns of chironomid-inferred temperature changes followed the meteorological record over the last ∼150years (r Pearson=0.65, P=0.01) and 87% of the inferences had deviations from the instrumental data below the root-mean-square error of prediction (RMSEP). When the inferences were compared with a 2-year running mean in the meteorological data, 94% of the inferences had differences with the instrumental data below the RMSEP, indicating that more than half of the inaccurate inferences may have been due to errors in varve counting. Larger deviations from the instrumental data were also obtained from samples with low percentages of fossil taxa represented in the training set used for temperature reconstruction and/or assemblages with poor fit to temperature. Changes in total phosphorus (TP, as inferred by diatoms) and/or greater precipitation were possible factors affecting the accuracy of the temperature reconstruction. Although these factors might affect the quantitative estimates, obtaining >80% accurate temperature inferences suggests that chironomid analysis is a reliable tool for reconstructing mean July air temperature quantitatively over the last ∼150years in Lake Silvaplan

Response of Lacustrine Biota to Late Holocene Climate and Environmental Conditions in Northernmost Ungava (Canada)

Sediment cores from three lakes located in the northernmost region of Ungava, Québec (Canada) were examined to define aquatic community and ecosystem variability during the Late Holocene period. A chironomid-based transfer function was used to reconstruct August air temperature trends, and lacustrine primary production was inferred from sedimentary biogenic silica content and siliceous microfossil abundances. Trends in primary production, sediment organic matter content (estimated through loss on ignition), and chironomid-inferred temperature were compared to explore potential effects of environmental change on biotic assemblage composition at centennial to millennial time scales. Although no direct correlation between chironomid-inferred August air temperature and primary production was observed, we found indications that both chironomid and diatom communities were responding to the same overarching regional climatic and environmental processes. Over the last decade, northern Québec has been undergoing notable, rapid warming that contrasts with the relative inertia of the past few millennia. This study provides a baseline against which recent and future environmental changes in this region can be compared. Les archives sédimentaires couvrant la période de l’Holocène tardif ont été examinées dans trois lacs situés dans la région du nord de l’Ungava, au Québec (Canada). Un modèle d’inférence basé sur les assemblages de chironomides a été utilisé pour reconstruire la variabilité des températures de l’air du mois d’août, et la production primaire lacustre a été inférée par le contenu sédimentaire en silice biogénique et les abondances des microfossiles siliceux. Les variations historiques de la production primaire, du contenu organique du sédiment (évalué par la perte au feu) et les températures inférées ont été comparées afin d’explorer les effets potentiels des changements environnementaux sur la composition des assemblages à différentes échelles temporelles (centenaires à millénaires). Malgré le fait qu’aucune corrélation directe n’ait été observée entre les températures inférées en août et la productivité primaire, certaines indications suggèrent que les communautés de chironomides et de diatomées répondaient aux mêmes processus climatiques et environnementaux régionaux. Au cours de la dernière décennie, le nord du Québec a connu un réchauffement rapide et marqué, contrastant avec l’inertie relative des derniers millénaires. Cette étude fournit le scénario de référence par rapport auquel les changements environnementaux actuels et futurs pourront être comparés dans cette région

Immunogenicity of a Killed Bivalent (O1 and O139) Whole Cell Oral Cholera Vaccine, Shanchol, in Haiti

Background: Studies of the immunogenicity of the killed bivalent whole cell oral cholera vaccine, Shanchol, have been performed in historically cholera-endemic areas of Asia. There is a need to assess the immunogenicity of the vaccine in Haiti and other populations without historical exposure to Vibrio cholerae. Methodology/Principal Findings We measured immune responses after administration of Shanchol, in 25 adults, 51 older children (6–17 years), and 47 younger children (1–5 years) in Haiti, where cholera was introduced in 2010. A≥4-fold increase in vibriocidal antibody titer against V. cholerae O1 Ogawa was observed in 91% of adults, 74% of older children, and 73% of younger children after two doses of Shanchol; similar responses were observed against the Inaba serotype. A≥2-fold increase in serum O-antigen specific polysaccharide IgA antibody levels against V. cholerae O1 Ogawa was observed in 59% of adults, 45% of older children, and 61% of younger children; similar responses were observed against the Inaba serotype. We compared immune responses in Haitian individuals with age- and blood group-matched individuals from Bangladesh, a historically cholera-endemic area. The geometric mean vibriocidal titers after the first dose of vaccine were lower in Haitian than in Bangladeshi vaccinees. However, the mean vibriocidal titers did not differ between the two groups after the second dose of the vaccine. Conclusions/Significance: A killed bivalent whole cell oral cholera vaccine, Shanchol, is highly immunogenic in Haitian adults and children. A two-dose regimen may be important in Haiti, and other populations lacking previous repeated exposures to V. cholerae

Context and Cardiovascular Risk Modification in Two Regions of Ontario, Canada: A Photo Elicitation Study

Cardiovascular diseases, which include coronary heart diseases (CHD), remain the leading cause of death in Canada and other industrialized countries. This qualitative study used photo-elicitation, focus groups and in-depth interviews to understand health behaviour change from the perspectives of 38 people who were aware of their high risk for CHD and had received information about cardiovascular risk modification while participating in a larger intervention study. Participants were drawn from two selected regions: Sudbury and District (northern Ontario) and the Greater Toronto Area (southern Ontario). Analysis drew on concepts of place and space to capture the complex interplay between geographic location, sociodemographic position, and people’s efforts to understand and modify their risk for CHD. Three major sites of difference and ambiguity emerged: 1) place and access to health resources; 2) time and food culture; and 3) itineraries or travels through multiple locations. All participants reported difficulties in learning and adhering to new lifestyle patterns, but access to supportive health resources was different in the two regions. Even within regions, subgroups experienced different patterns of constraint and advantage. In each region, “fast” food and traditional foods were entrenched within different temporal and social meanings. Finally, different and shifting strategies for risk modification were required at various points during daily and seasonal travels through neighbourhoods, to workplaces, or on vacation. Thus health education for CHD risk modification should be place-specific and tailored to the needs and resources of specific communities