Use of in vitro and haptic assessments in the characterisation of surface lubricity

Lubricity is a key property of hydrophilic-coated urinary catheter surfaces. In vitro tests are commonly employed for evaluation of surface properties in the development of novel catheter coating technologies, however, their value in predicting the more subjective feeling of lubricity requires validation. We herein perform a range of in vitro assessments and human organoleptic studies to characterise surface properties of developmental hydrophilic coating formulations, including water wettability, coefficient of friction, dry-out kinetics and lubricity. Significant reductions of up to 40% in the contact angles and coefficient of friction values of the novel coating formulations in comparison to the control poly(vinyl pyrrolidone)-coated surfaces were demonstrated during quantitative laboratory assessments. In contrast, no significant differences in the more subjective feeling of lubricity between the novel formulations and the control-coated surfaces were observed when formulations were haptically assessed by the techniques described herein. This study, importantly, highlights the need for optimisation of in vitro and human haptic assessments to more reliably predict patient preferences

On Generalisation of Dual-Thermocouple Sensor Characterisation to RTDs

Intrusive temperature sensors such as thermocouples and resistance temperature detectors (RTDs) have become industry standards for simple and cost-effective temperature measurement. However, many situations require the use of physically robust and therefore low bandwidth temperature sensors. Much work has been published on dual-thermocouple thermometry as a means of obtaining increased sensor bandwidth from relatively robust thermocouples, which are assumed to have firstorder response. This contribution seeks to determine if RTDs, which are known to have approximately first-order response [1], can also be characterised using the dual-thermocouple approach. Experimental results show that the response of an RTD cannot be represented by a first-order model with sufficient accuracy to allow successful application of this method. Furthermore, simulation studies demonstrated that if a sensor exhibits even marginally second-order response, highly inaccurate temperature reconstructions follow. It is concluded that a higher-order model that more accurately reflects RTD response would be required for successful dual-RTD characterisation

Turn up, tune in, don’t drop out : the relationship between lecture attendance, use of lecture recordings, and achievement at different levels of study

Open Access via Springer CompactPeer reviewedPublisher PD

In Situ Two-Thermocouple Sensor Characterisation using Cross-Relation Blind Deconvolution with Signal Conditioning for Improved Robustness

Thermocouples are one of the most widely used temperature measurement devices due to their low cost, ease of manufacture and robustness. However, their robustness is obtained at the expense of limited sensor bandwidth. Consequently, in many applications signal compensation techniques are needed to recover the true temperature from the attenuated measurements. This, is turn, necessitates in situ thermocouple characterisation. Recently the authors proposed a novel characterisation technique based on the cross-relation method of blind deconvolution applied to the output of two thermocouples simultaneously measuring the same temperature. This offers a number of advantages over competing methods including low estimation variance and no need for a priori knowledge of the time constant ratio. A weakness of the proposed method is that it yields biased estimates in the presence of measurement noise. In this paper we propose the inclusion of a signal conditioning step in the characterisation algorithm to improve the robustness to noise. The enhanced performance of the resulting algorithm is demonstrated using both simulated and experimental data

Probing non-spherical dark halos in the Galactic dwarf galaxies

We construct axisymmetric mass models for dwarf spheroidal (dSph) galaxies in the Milky Way to obtain plausible limits on the non-spherical structure of their dark halos. This is motivated by the fact that the observed luminous parts of the dSphs are actually non-spherical and Cold Dark Matter (CDM) models predict non-spherical virialized dark halos. Our models consider velocity anisotropy of stars $\bar{v^2_R} / \bar{v^2_{\phi}}$, which can vary with the adopted cylindrical coordinates under the assumption $\bar{v^2_z}=\bar{v^2_R}$ for simplicity, and also include an inclination of the system as a fitting parameter to explain the observed line-of-sight velocity dispersion profile. Applying these models to six of the bright dSphs in the Milky Way, we find that the best-fitting cases for most of the dSphs yield oblate and flattened dark halos, irrespective of assumed density profiles in their central parts. We also find that the total mass of the dSphs enclosed within a spheroid with major-axis length of 300 pc varies from $10^6M_{\odot}$ to $10^7M_{\odot}$, contrary to the conclusion from spherical models. This suggests the importance of considering shapes of dark halos in mass models of the dSphs. It is also found that dark halos of the Galactic dSphs may be more flattened than N-body predictions, thereby implying our yet incomplete understanding of baryonic and/or non-baryonic dark matter physics in dwarf galaxy scales.Comment: 13 pages, 9 figures, Accepted for publication in Ap

The Recent Star Formation History of NGC 5102

We present Hubble Space Telescope photometry of young stars in NGC 5102, a nearby gas-rich post-starburst S0 galaxy with a bright young stellar nucleus. We use the IAC-pop/MinnIAC algorithm to derive the recent star formation history in three fields in the bulge and disk of NGC 5102. In the disk fields, the recent star formation rate has declined monotonically and is now barely detectable, but a starburst is still in progress in the bulge and has added about 2 percent to the mass of the bulge over the last 200 Myr. Other studies of star formation in NGC 5102 indicate that about 20 percent of its stellar mass was added over the past Gyr. If this is correct, then much of the stellar mass of the bulge may have formed over this period. It seems likely that this star formation was fueled by the accretion of a gas-rich system with HI mass of about 2 x 10^9 Msol which has now been almost completely converted into stars. The large mass of recently formed stars and the blue colours of the bulge suggest that the current starburst, which is now fading, may have made a significant contribution to build the bulge of NGC 5102.Comment: 36 pages, 16 figures, accepted in A

Gene-Environment Interaction in the Onset of Eczema in Infancy: Filaggrin Loss-of-Function Mutations Enhanced by Neonatal Cat Exposure

Background Loss-of-function variants in the gene encoding filaggrin (FLG) are major determinants of eczema. We hypothesized that weakening of the physical barrier in FLG-deficient individuals may potentiate the effect of environmental exposures. Therefore, we investigated whether there is an interaction between FLG loss-of-function mutations with environmental exposures (pets and dust mites) in relation to the development of eczema. Methods and Findings We used data obtained in early life in a high-risk birth cohort in Denmark and replicated the findings in an unselected birth cohort in the United Kingdom. Primary outcome was age of onset of eczema; environmental exposures included pet ownership and mite and pet allergen levels. In Copenhagen(n = 379), FLG mutation increased the risk of eczema during the first year of life (hazard ratio [HR] 2.26, 95% confidence interval [CI] 1.27–4.00, p = 0.005), with a further increase in risk related to cat exposure at birth amongst children with FLG mutation (HR 11.11, 95% CI 3.79–32.60, p < 0.0001); dog exposure was moderately protective (HR 0.49, 95% CI 0.24–1.01, p = 0.05), but not related to FLG genotype. In Manchester (n = 503) an independent and significant association of the development of eczema by age 12 mo with FLG genotype was confirmed (HR 1.95, 95% CI 1.13–3.36, p = 0.02). In addition, the risk increased because of the interaction of cat ownership at birth and FLG genotype (HR 3.82, 95% CI 1.35–10.81, p = 0.01), with no significant effect of the interaction with dog ownership (HR 0.59, 95% CI 0.16–2.20, p = 0.43). Mite-allergen had no effects in either cohort. The observed effects were independent of sensitisation. Conclusions We have demonstrated a significant interaction between FLG loss-of-function main mutations (501x and 2282del4) and cat ownership at birth on the development of early-life eczema in two independent birth cohorts. Our data suggest that cat but not dog ownership substantially increases the risk of eczema within the first year of life in children with FLG loss-of-function variants, but not amongst those without. FLG-deficient individuals may need to avoid cats but not dogs in early life

Anti-Adherent Biomaterials for Prevention of Catheter Biofouling

Medical device-associated infections present a leading global healthcare challenge, and effective strategies to prevent infections are urgently required. Herein, we present an innovative anti-adherent hydrogel copolymer as a candidate catheter coating with complementary hydrophobic drug-carrying and eluting capacities. The amphiphilic block copolymer, Poloxamer 188, was chemically-derivatized with methacryloyl moieties and copolymerized with the hydrogel monomer, 2-hydroxyethyl methacrylate. Performance of the synthesized copolymers was evaluated in terms of equilibrium swelling, surface water wettability, mechanical integrity, resistance to encrustation and bacterial adherence, and ability to control release of the loaded fluoroquinolone antibiotic, ofloxacin. The developed matrices were able to provide significant protection from fouling, with observed reductions of over 90% in both adherence of the common urinary pathogen Escherichia coli and encrusting crystalline deposits of calcium and magnesium salts relative to the commonly employed hydrogel, poly (hydroxyethyl methacrylate). Additionally, the release kinetics of a loaded hydrophobic drug could be readily tuned through facile manipulation of polymer composition. This combinatorial approach shows significant promise in the development of suitable systems for prevention of catheter-associated infections

Photochemically controlled drug dosing from a polymeric scaffold

Purpose To develop the first photoactive biomaterial coating capable of controlled drug dosing via inclusion of synthesised drug-3,5-dimethoxybenzoin (DMB) conjugates in a poly(2-methyoxyethyl acrylate) (pMEA) scaffold. Methods Flurbiprofen- and naproxen-DMB conjugates were prepared via esterification and characterised via NMR spectroscopy and mass spectrometry following chromatographic purification. Conjugate photolysis was investigated in acetonitrile solution and within the pMEA matrix following exposure to low-power 365 nm irradiation. Photo-liberation of drug from pMEA into phosphate buffered saline was monitored using UV-vis spectroscopy. Results The synthetic procedures yielded the desired drug conjugates with full supporting characterisation. Drug regeneration through photolysis of the synthesised conjugates was successful in both acetonitrile solution and within the pMEA scaffold upon UV irradiation. Conjugates were retained within the pMEA scaffold with exclusive drug liberation following irradiation and increased drug dose with increasing exposure. Multi-dosing capacity was demonstrated though the ability of successive irradiation periods to generate further bursts of drug. Conclusion This study demonstrates the first application of photochemically controlled drug release from a biomaterial coating and the feasibility of using pMEA as a scaffold for housing the photoactive drug-DMB conjugates