Characterization of G -Semigroup by Intuitionistic N-Fuzzy Set (INFS) and its level set

Some characterizations of G -Semigroup by intuitionistic N-fuzzy sets have been given here. The concept of intuitionistic N-fuzzy set (INFS) and its level set has been applied to G -semigroup. The notions of intuitionistic N-fuzzy G -subsemigroup and intuitionistic N-fuzzy G - ideals (left, right, lateral, quasi, and bi) have been introduced and characterized by intuitionistic N-fuzzy sets

Molecular Identification of Genetically Modified Crops for Biosafety and Legitimacy of Transgenes

Crops undergo artificially DNA modifications for improvements are considered as genetically modified (GM) crops. These modifications could be in indigenous DNA or by introduction of foreign DNA as transgenes. There are 29 different crops and fruit trees in 42 countries, which have been successfully modified for various traits like herbicide tolerance, insect/pest resistance, disease resistance and quality improvement. GM crops are grown worldwide and its area is significantly increasing every year. Many countries have very strict rules and regulations for GM crops and are also a trade barrier in some situations. Hence, identification and testing of crops for GM contents is important for identity and legitimacy of transgene to simplify the international trade. Normally, molecular identification is performed at three different levels, i.e., DNA, RNA and protein, and each level has its own importance in testing about the nature and type of GM crops. In this chapter, current scenario of GM crops and different molecular testing tools are described in brief

Prevalence of Substance Abuse Screening Of Adults by Pediatricians

As the heroin epidemic grips our country, teenage substance abuse in Punjab, much like other states, is increasing at alarming rates. Prevention and educational efforts as a result of screening and a focus on long term and sustainable goals of impacting the heroin epidemic need to be implemented now to have the greatest effect. In 2011, the American Academy of Pediatrics (AAP) issued a policy statement which recommends pediatricians provide instruction regarding substance abuse during their clinical interactions with adolescents. This study utilizes the Theoretical Domains Framework (TDF) through the use of a modified Determinants of Implementation Behavior Questionnaire (DIBQ) to survey pediatricians practicing within a rural region regarding substance abuse screening and intervention of their teenage patients. Use of TDF theory in the development of the DIBQ tool could reveal the specific challenges and barriers to substance abuse screening and the development and implementation of interventions useful in influencing the adoption of the AAP policy by pediatricians. The results of this study determined time as the largest barrier to compliance with the AAP policy, followed by confidentiality issues, limited resources for treatment, and patient level of honesty. All of the study respondents indicated awareness of and compliance with the AAP policy, most participants stated they had not been trained in the use of screening tools

DNA Fingerprinting and Cultivar Identification of Olive (Olea europaea L.) using SSR markers

Background: Genetic diversity and population structure of the olive varieties cultivated in Pakistan are yet to be explored.Methods: In present study, we studied population structure and genetic diversity and developed DNA fingerprints of 13 olive varieties  using 63 Simple Sequence Repeat  markers.Results: Collectively 618 alleles were amplified among which 582 were polymorphic and 36 were monomorphic. High allelic diversity per locus was found among 63 SSR markers, i.e., one for GAPU-12 to 23 for UDO099-008 and GAPU-47 with an average 9.80 alleles per locus. On the basis of cluster analysis genotypes were grouped into two clusters. Cluster I contained varieties Manzanilla, Sohawa-selection, Koroneki, Bulkasar-selection, Arbequina, Arbosona, Chugtai-selection, whereas cluster II was comprised of varieties Bari-Zatoon, Coratina, Gemlik, Frontaio, Pendolino and Ottobratica. UDO-24 marker alone identified seven olive varieties. Similarly, DCA-07 and EMO-02 identified six olive varieties each.Conclusion: The findings of this manuscript will be helpful for future studies related to DNA fingerprinting and genetic diversity assessment for choice of SSR markers and identification of olive varieties.   Keywords: Cluster analysis; Polymorphic Information Content; Structure analysis; Similarity matrix

Is Accounting Information Produced by the Firms any Value Relevant?

This research is motivated by dearth of research on the impact of accounting information produced by Pakistani companies on their share prices and is aimed at highlighting the useful ness of accounting information in investment decision making by investors. Top five companies from each of the sectors, excluding the financial services sectors listed on Pakistan Stock Exchange are studied for the years 2006 to 2016. Panel data settings are used to study the impact of accounting information i.e., Earnings on Returns. Three control variables namely, size, profitability and industry type are added in Extended returns model to capture the impact of accounting information on returns. The results show that accounting information i.e., Earnings and Changes in Earnings and profitability are value relevant but the size and industry type have no impact on value relevance

Protocol optimization for efficient in vitro micro-propagation of Stevia rebaudiana Bertoni

Background: Stevia rebaudiana Bertoni is one of the most amazing herb that is an abundant source of diterpene glycoside a non-caloric sweetener. In recent years alongside its utilization in herbal and folk medicine, commercial exploitation of this herb for weight control and anti-diabetic treatment has started.Methods: In present study healthy nodal segments of Stevia rebaudiana Bertoni were used as explants for optimization of in vitro micro-propagation and regeneration protocol for rapid, fast and efficient multiplication under in-vitro conditions. For shoot proliferation and rooting various concentrations cytokinins NAA (Naphthalene acetic acid), BAP (Benzyl amino purine), IAA (Indole acetic acid) and kinetin (Kn) were employed.Results: Shoots without roots were regenerated from nodal segments via auxiliary shoot induction. The production of multiple shoots from nodal portion was maximum on MS medium complemented with 0.5 mg/L of BAP and 1.5 mg/L of Kn. Maximum rooting was recorded on MS medium supplemented with 0.25 mg/L NAA and 1.0 mg/L of IAA. Successful hardening was achieved under controlled growth room conditions followed by transfer to glass house and open field.Conclusion: Standardization of explant source and culturing media for multiplication of shoot resulted in mass proliferation of homogenous and healthy plantlets of Stevia clones under local conditions. Keywords: Stevia rebaudiana Bertoni; BAP; Kinetin, NAA; IAA; Micro-propagation; Regeneratio

Adaptability studies of garlic (Allium sativum) advanced lines

The purpose of this research was to identify the most desirable verities adopted in different environmental conditions of the country. Five garlic advance line; NARC Garlic-1, NARC Garlic-3, NARC Garlic-4, NARC Garlic-5 along with ‘Lehsan Gulabi’ as check were evaluated at different locations along with NARC, Islamabad. Different morphological parameters; plant height, plant girth, leaf number per plant and leaf width were studied at four different growth stages. NARC Garlic-1 performed better as compared other advance lines and control tested

Molecular Markers and Cotton Genetic Improvement: Current Status and Future Prospects

Narrow genetic base and complex allotetraploid genome of cotton (Gossypium hirsutum L.) is stimulating efforts to avail required polymorphism for marker based breeding. The availability of draft genome sequence of G. raimondii and G. arboreum and next generation sequencing (NGS) technologies facilitated the development of high-throughput marker technologies in cotton. The concepts of genetic diversity, QTL mapping, and marker assisted selection (MAS) are evolving into more efficient concepts of linkage disequilibrium, association mapping, and genomic selection, respectively. The objective of the current review is to analyze the pace of evolution in the molecular marker technologies in cotton during the last ten years into the following four areas: (i) comparative analysis of low- and high-throughput marker technologies available in cotton, (ii) genetic diversity in the available wild and improved gene pools of cotton, (iii) identification of the genomic regions within cotton genome underlying economic traits, and (iv) marker based selection methodologies. Moreover, the applications of marker technologies to enhance the breeding efficiency in cotton are also summarized. Aforementioned genomic technologies and the integration of several other omics resources are expected to enhance the cotton productivity and meet the global fiber quantity and quality demands

Characterization of G -Semigroup by Intuitionistic N-Fuzzy Set (INFS) and its Level Set

Some characterizations of G -Semigroup by intuitionistic N-fuzzy sets have been given here. The concept of intuitionistic N-fuzzy set (INFS) and its level set has been applied to G -semigroup. The notions of intuitionistic N-fuzzy G -subsemigroup and intuitionistic N-fuzzy G - ideals (left, right, lateral, quasi, and bi) have been introduced and characterized by intuitionistic N-fuzzy sets