Azadirachta indica induced suppression of Mycobacterium tuberculosis secreted proteins in human monocyte

The H37Rv strain of MTB was grown in modified Sauton’s medium till mid-log phase. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient sedimentation on Ficoll-Paque separation medium. Adherent monocytes obtained from PBMC’s were infected with H37Rv for 90 min. in the ratio of 1:1 (1 bug: 1 cell). Thereafter, after washing, infected cells were co-cultured with varying doses of neem extract for 24 hrs, and harvested subsequently. Modulation of secreted TNF-[alpha], iNOS and MTB Ag85 complex expressions in culture supernatants was estimated by ELISA.
We report the high basal expression of secreted tumor necrosis factor-[alpha] (TNF-[alpha]), inducible nitric oxide synthase (iNOS) and MTB Ag85 complex in MTB-infected monocytes was suppressed by neem extract in a dose-dependent manner (P<0.001 for all). A concentration of 1ug/ml of neem extract showed a suppression by ~ 2.5-fold (P<0.001), 1.7-fold (P<0.001) and 1.8-fold (P<0.001) in the expressions of
secreted TNF-[alpha], iNOS and MTB Ag85 respectively in 24 hr culture supernatants of MTB-infected monocytes.
Thus, neem extract seems to be a potential future adjunct for in-depth studies in the management of tuberculosis

Economic Cost of Road Traffic Accidents in Twin Cities, Pakistan

Road Traffic Accidents in developing countries are a serious concern in terms of safety and economy. RTAs share a considerable amount of overall life, resource, and productivity losses. It has become essential to monetize RTAs to aid the policymakers, economists, and the state government to efficiently select the cost-effective countermeasure for the safety of road users. The costs can be utilized in framing cost-effective safety measures and policies. This paper focuses on estimating the overall costs of Road Traffic Accidents in the city of Rawalpindi/Islamabad, Pakistan, by using the Human Capital Approach to estimate the monetary amount that the Road Traffic Accidents have cost to the cities in the year 2016. The research has categorized the costs into 5 main components, namely: medical costs, property damage costs, loss output, human loss, and administrative costs. A total of 10187 incidents have been recorded in the cities involving 12573 victims and 18136 vehicles. Results suggested that a total of pkr 2.183 billion has been incurred by the State of Pakistan with a loss of about 0.0074% to the GDP. The breakdown of the costs was as follows: Medical costs (pkr 93,898,640), and it comprises 4.26% of the total cost; Property damage costs include vehicular and property damage repair (pkr 214,840,463), and it comprises 9.84% of the total cost; Loss output (pkr 1,316,000,000), and it comprises 60.27% of the total cost; Human costs (pkr 473,584,345), and it comprise 21.69% of the total cost; Administrative costs (pkr 85,730,725), and it comprises 3.92% of the total cost

Antioxidative activity of clove (syzygium aromaticum) oil administration in Middle cerebral artery occlusion (mcao) Models of acute focal cerebral ischemia

Stroke is a multifactorial neurological deficit syndrome. Oxidative stress is the principal underlying pathophysiological mechanism of ischemic stroke associated with neuronal damage and neuroinflammation

Polymorphism of HLA-DR and HLA-DQ in rheumatoid arthritis patients and clinical response to methotrexate--a hospital-based study

Objective: To investigate the frequency and distribution of DRB1 and DQB1 alleles in Patients with rheumatoid arthritis (RA) and analyze the relationship between clinical response to methotrexate (MTX) and the HLA-DR and HLA-DQ genotypes in these patients.Methods: In this case-control study, the HLA-DRB1 and HLA-DQB1 polymorphism in 91 RA patients and 91 healthy controls was done using polymerase chain reaction and sequence specific primers.Results: There was no statistical difference in frequencies of HLA-DRB1*03, DRB1*04, DRB1*07, DRB1*10, DRB1*11, DRB1*12, DRB1*13, DRB1*14, DRB1*15 and DRB1*16 genotypes between patients and controls. However, DRB1*01 was found to be significantly more common (p=0.015) in RA patients compared to controls. HLA-DRB1*15 was more common in patients (43.5%) compared to controls (30.8%) but results were not significant. HLA-DRB1*08 and DRB1*09 were present in negligible number in patients as well as controls while HLA-DRB1*12 was conspicuously absent in controls. Similarly, DQB1*06 was also significantly more common (p = 0.01) among the patients compared to healthy control subjects, while there was no statistical difference in the frequencies of DQB1*02, DQB1*03, DQB1*04 and DQB1*05 among the cases and the controls. RA susceptibility in most patients appeared to be associated with the HLA-DRB1*01/DQB1 *06 genotype. Regarding association between HLA-DR or HLA-DQ genotype and clinical response to methotrexate (MTX), the data showed that with the exception of HLA-DRB1*03, there appears to be no association between the particular subtypes of HLA-DR and HLA-DQ. HLA-DRB1*03 was significantly-more common among non-responders to MTX alluding to the possibility that another genes responsible for MTX metabolism, might be in linkage disequilibrium with HLA-DRB1*03 in the Pakistani population, thereby making such individuals non-responsive to MTX-therapy.Conclusion: RA susceptibility in most Pakistani patients is associated with the HLA-DRB1*01/DQB1*06 genotype. HLA-DRB1*03 was found to be significantly more common among non-responders to MTX treatment suggesting that Pakistani patients with this genotype are less likely to benefit from MTX

GENDER MAINSTREAMING IN TO COMMUNITY BASED DISASTER RISK MANAGEMENT

Although women are considered as the most vulnerable group in the society; but very little attention has been made to take into consideration the issue of gender sensitivity during the phase of Community Based Disaster Risk Reduction as well as Management in both natural and manmade disaster. In Pakistan a very limited number of organization are working to address the issue of mainstreaming of women in pre and post disaster activities. The goal of this paper is to synthesize and review the issue of disaster and gender mainstreaming. This paper highlights the gaps in terms of disaster preparedness by adopting CBDRM; and also critically analyzes the importance of the mainstreaming the element of gender in the phase of Disaster Mainstreaming overall in general and specific in the context of Pakistan. Recommendations and suggestions of the paper can be used to design and implement comprehensive CBDRM Preparedness Plan by mainstreaming the element of Gender sensitivity

IMPACTS OF ATTABAD LAKE (PAKISTAN) AND ITS FUTURE OUTLOOK

Due to geological, hydrological and anthropogenic variations in Northern Areas of Pakistan, the possibility of hazards like earthquake, flash floods, landslide and Glacial Lake Outburst Flood (GLOF) have increased manifolds. The incident of Attabad landslide on 4th January 2010 blocking the Hunza River and resulting in the formation of huge lake as a sequel to Astore earthquake in 2002 and Kashmir earthquake in 2005 is a testimony to the fact hazards turn into disasters causing loss and damage to lives, properties, assets and environment. There are multiple reasons ranging from global warming, deforestation, unplanned settlements, population growth, aggressive farming and host of other man induced activities which have made the Northern Areas of Pakistan hazards prone and are likely to pose continuous threat if requisite preparation and response options are not considered well in advance. In case of Attabad landslide, viewing from the prism of disaster risk management the response of Gilgit-Baltistan and Federal Governments has though not been up to the mark yet mobilization of resources and opinion to manage the disaster leaves a promising environment of hope and capacity which can be further developed to more resilient respondent provided little extra deliberations are done and attention is paid. The solution to Attabad landslide dam crisis lies in reducing the risk by either draining out the lake completely or making a reliable structure with proper design for flow of the water from the lake

Vitamin D Deficiency in HCV Antiviral Treatment Responders versus Non-Responders

Background: Hepatitis C virus (HCV) is a major cause of chronic liver disease (CLD). Pakistan has a high burden of infectious diseases, including HCV. Its prevalence varies according to geographic regions in the country from about 2·4% to 6·5%. The objective of the study was to compare the frequency of vitamin D deficiency in responders and non-responders of antiviral treatment for chronic hepatitis C.Material and Methods: This comparative cross-sectional study was conducted in Hepatitis Clinic, Jinnah hospital, Lahore from 20th May to 20th November 2013. After ethical approval, participants were selected by using purposive non-probability sampling, 52 responder patients i.e. who were labeled negative for HCV RNA by PCR after 12 weeks of antiviral treatment and 52 non-responder patients were included in this study. Data was collected by using pretested structured questionnaire. Vitamin D3 levels were measured by ELISA and a cut-off value of below 30ng/ml was labeled as Vitamin D deficiency. SPSS version 21 was used to analyze data with p value less than 0.05 taken as statistically significant.Results: Out of 104 patients (mean age 35±8.1 years), 61.5% were males and 38.5 % were females. There was a significant difference in frequency of vitamin D deficiency in treatment responder group when compared to non-responders (p = 0.016). Mean level of vitamin D was 21.8±10.8ng/ml in responders whereas it was 15.6±7.5 in non-responders with a statistically significant difference (p = 0.001).Conclusion: This study concludes that there is a significant vitamin D deficiency among treatment non-responders as compared to treatment responders in patients with chronic hepatitis C

Comparing the Effects of Choline with Clozapine and Fluoxetine for Improving Cognitive Behavior in Rats

Background: Cognitive behavior therapy is an important treatment for various psychiatric and psychological problems. Different psychotherapeutic treatments are used for improvement in patients. The study aimed to compare Clozapine and Fluoxetine with Choline on the progress of cognition and cognitive behavior in rats. Methods: This experimental study was conducted in the pharmacology department of Karachi University on locally bred male albino rats (n=24). These were divided into four treatment groups (Saline, Fluoxetine, Clozapine, and Choline) and measured the output at the 1st, 3rd, 5th, and 7th weeks. Familiar and Novel object recognition test and Passive avoidance test was used to observe learning and memory as well as the mechanism of cognition. One way-ANOVA and post-hoc analysis was done between groups. The p-value <0.05 and < 0.001 were considered statistically significant and highly significant respectively. Results: The comparative mean preference index percentage between saline, choline, clozapine, and fluoxetine at week one was non–significant (p>0.05) in the Novel and Familiar Object Recognition test. However, at week three it was highest for Fluoxetine (58.15±3.35) compared to Choline, Clozapine and Saline for the novel object. However, in Familiar objects, it was found highest for Clozapine (58.88±3.05) (p <0.05). Furthermore, the mean step-through latency time of the Passive Avoidance test was significant (p<0.05) at weeks three, five and seven. It was highest for choline (92.5±1.36) than fluoxetine and clozapine. Conclusion: Fluoxetine has a significant effect (p<0.001) on memory and learning compared to Clozapine. Clozapine and choline showed statistically same results on cognitive behavior.  Keywords: Cognitive Behavior; Choline; Clozapine; Fluoxetine; Effect; Compare.

Smoking and blood pressure levels in hypertensive patients: evaluating the role of gender, age and hypertension duration

Background: Hypertension is estimated to affect about 40% of adults above 25 years of age globally. While many of the risk factors of hypertension are well known, such is not the case with smoking. Pertinent published literature is heterogeneous, with a dearth of relevant local data. Objectives was to study the relationship between smoking and blood pressure levels in hypertensive patients and to assess the effects of gender, age and hypertension duration on such a relationship.Methods: A cross-sectional study was carried out on a total of 298 conveniently sampled patients, aged 18 or above, from the medical outpatient department of a secondary care hospital of Karachi. The relevant data were collected by means of a structured questionnaire whereas the blood pressure level was checked using sphygmomanometer with stethoscope. Mann Whitney U test was used for inferential analysis whereas the significance level was set at 0.05.Results: Overall, both the systolic and diastolic blood pressure levels were significantly associated with smoking history (p<0.05 for both) where they were found to be higher in smokers than in non-smokers. Furthermore, after gender, age and hypertension duration based stratifications; both the systolic and diastolic blood pressure levels were still significantly associated with smoking history in patients who were male, were ≥35 years old and had <5 years hypertension duration (p<0.05 for both) where they were again found to be higher in smokers than in non-smokers.Conclusions: The study results showed a significant association of positive smoking history with higher mean systolic and diastolic blood pressure levels, though after stratifying for gender, age and hypertension duration, this relationship persisted only in patients who were male, were ≥35 years old and had <5 years hypertension duration