Body weight gain/loss trend of male rats with obesity induced by high fat-diet (HFD) during the Sango sprout juice (SSJ) intervention period.

<p><b>Animals were treated as follows.</b> Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with vehicle. Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 15 mg/kg b.w. SSJ. Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 75 mg/kg b.w. SSJ. Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 150 mg/kg b.w. SSJ. Values are mean ± standard error of the mean (S.E.M.) *<i>P</i><0.05; **<i>P</i><0.01 (two-tailed unpaired t-test).</p

Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on antioxidant enzyme activities in the liver of male rats with obesity induced by high fat-diet (HFD).

<p>Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on antioxidant enzyme activities in the liver of male rats with obesity induced by high fat-diet (HFD).</p

Effects of Sango sprout juice (SSJ) on liver weight of male rats with obesity induced by high fat-diet (HFD) and treated as follows.

<p>Rats fed RD and treated by gavage daily for twenty-eight consecutive days with vehicle. Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with vehicle. Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 15 mg/kg b.w. SSJ. Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 75 mg/kg b.w. SSJ. Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 150 mg/kg b.w. SSJ. The bars show mean values ± standard error of the mean (S.E.M.) of seven measurements performed on seven rat samples for each studied group. Mean values were significantly different compared with the HFD group (two-tailed unpaired t-test): *<i>P</i><0.05, **<i>P</i><0.01. Mean values were significantly different compared with the RD group (two-tailed unpaired t-test): ^†<i>P</i><0.05, ^††<i>P</i><0.01.</p

Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on bodyweight gain/loss, liver weight, and daily food intake of male rats with obesity induced by high fat-diet (HFD).

<p>Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on bodyweight gain/loss, liver weight, and daily food intake of male rats with obesity induced by high fat-diet (HFD).</p

Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on bodyweight gain/loss, liver weight, and daily food intake of male rats with obesity induced by high fat-diet (HFD).

<p>Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on bodyweight gain/loss, liver weight, and daily food intake of male rats with obesity induced by high fat-diet (HFD).</p

Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on phase I enzyme activities in the liver of male rats with obesity induced by high fat-diet (HFD).

<p>Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on phase I enzyme activities in the liver of male rats with obesity induced by high fat-diet (HFD).</p

Representative macroscopic pictures of the liver from different experimental groups.

<p><b>A</b>: Rats fed RD and treated by gavage daily for twenty-eight consecutive days with vehicle (RD). <b>B</b>: Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with vehicle (HFD). <b>C</b>: Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 15 mg/kg b.w. SSJ (HFD+15 mg/kg b.w. SSJ). <b>D</b>: Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 75 mg/kg b.w. SSJ (HFD+75 mg/kg b.w. SSJ). E: Rats fed HFD and treated by gavage daily for twenty-eight consecutive days with 150 mg/kg b.w. SSJ (HFD+150 mg/kg b.w. SSJ).</p

Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on phase II enzyme activities in the liver of male rats with obesity induced by high fat-diet (HFD).

<p>Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on phase II enzyme activities in the liver of male rats with obesity induced by high fat-diet (HFD).</p

Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on lipid profile in the serum of male rats with obesity induced by high fat-diet (HFD).

<p>Effects of <i>Raphanus sativus</i> cv. Sango sprout juice (SSJ) on lipid profile in the serum of male rats with obesity induced by high fat-diet (HFD).</p

<i>Raphanus sativus</i> cv. Sango Sprout Juice Decreases Diet-Induced Obesity in Sprague Dawley Rats and Ameliorates Related Disorders

<div><p>Background</p><p>Obesity is recognized as a leading global health problem, correlated with an increased risk for several chronic diseases. One strategy for weight control management includes the use of vegetables rich in bioactive compounds to counteract weight gain, improve the antioxidant status and stimulate lipid catabolism.</p><p>Aim of the Study</p><p>The aim of this study was to investigate the role of <i>Raphanus sativus</i> Sango sprout juice (SSJ), a Brassica extraordinarily rich in anthocyanins (AC) and isothiocyanates (ITCs), in a non-genetic model of obesity (high fat diet-HFD induced).</p><p>Methods</p><p>Control groups were fed with HFD or regular diet (RD). After a 10-week period, animals were assigned to experimental units and treated by gavage for 28 days as follows: HFD and RD control groups (rats fed HFD or RD and treated with vehicle only) and HFD-treated groups (rats fed HFD and treated with 15, 75 or 150 mg/kg b.w. of SSJ). Body weight and food consumption were recorded and serum lipid profile was measured (total cholesterol, triglycerides, and non-esterified fatty acids). Hepatic phase-I, phase-II as well as antioxidant enzymatic activities were assessed.</p><p>Results</p><p>SSJ lowered total cholesterol level, food intake and liver weight compared with HFD rodents. SSJ at medium dose proved effective in reducing body-weight (~19 g reduction). SSJ was effective in up-regulating the antioxidant enzymes catalase, NAD(P)H:quinone reductase, oxidised glutathione reductase and superoxide dismutase, which reached or exceeded RD levels, as well as the phase II metabolic enzyme UDP-glucuronosyl transferase (up to about 43%). HFD up-regulated almost every cytochrome P450 isoform tested, and a mild down-regulation to baseline was observed after SSJ intervention.</p><p>Conclusion</p><p>This work reveals, for the first time, the antioxidant, hypolipidemic and antiobesity potential of SSJ, suggesting its use as an efficient new functional food/nutraceutical product.</p></div