6 research outputs found
Improved medical education by systematic correlation of preclinical and clinical knowledge and skills
Using clustered data to develop biomass allometric models: The consequences of ignoring the clustered data structure
A comparison of different Gabor feature extraction approaches for mass classification in mammography
Transient Hoogsteen base pairs in canonical duplex DNA
Sequence-directed variations in the canonical DNA double helix structure that retain Watson-Crick base-pairing play important roles in DNA recognition, topology, and nucleosome positioning. By using nuclear magnetic resonance relaxation dispersion spectroscopy in concert with steered molecular dynamics simulations, we have observed transient sequence-specific excursions away from Watson-Crick base-pairing at CA and TA steps inside canonical duplex DNA towards low-populated and short-lived A•T and G•C Hoogsteen base-pairs. The observation of Hoogsteen base-pairs in DNA duplexes specifically bound to transcription factors and in damaged DNA sites implies that the DNA double helix intrinsically codes for excited state Hoogsteen base-pairs as a means of expanding its structural complexity beyond that which can be achieved based on Watson-Crick base-pairing. The methods presented here provide a new route for characterizing transient low-populated nucleic acid structures, which we predict will be abundant in the genome and constitute a second transient layer of the genetic code. Soon after its discovery1, it was recognized that the DNA double helix could accommodate a range of conformations that retain Watson-Crick (WC) base-pairing2. Sequence-directe
Effects of angiotensin-converting enzyme inhibition with perindopril on left ventricular remodeling and clinical outcome - Results of the randomized Perindopril and Remodeling in Elderly with Acute Myocardial Infarction (PREAMI) study
Background: Angiotensin-converting enzyme inhibitors
reduce mortality and remodeling after myocardial
infarction in patients with left ventricular dysfunction.
Methods: Perindopril and Remodeling in Elderly With
Acute Myocardial Infarction (PREAMI), a doubleblind,
randomized, parallel-group, multicenter, placebocontrolled
study, determined whether similar benefits occur
in elderly postinfarction patients with preserved left
ventricular function. A total of 1252 patients 65 years or
older with a left ventricular ejection fraction of 40% or
higher and recent acute myocardial infarction were randomized
to receive perindopril erbumine or placebo (8
mg/d) for 12 months. The combined primary end point
was death, hospitalization for heart failure, or left ventricular
remodeling. Secondary end points included cardiovascular
death, hospitalization for reinfarction or angina,
and revascularization.
Results: The primary end point occurred in 181 patients
(35%) taking perindopril and 290 patients (57%)
taking placebo, with a significant absolute risk reduction
of 0.22 (95% confidence interval, 0.16 to 0.28;
P.001). A total of 126 patients (28%) and 226 patients
(51%) in the perindopril and placebo groups, respectively,
experienced remodeling. The mean increase in left
ventricle end-diastolic volume was 0.7 mL with perindopril
compared with 4.0 mL with placebo (P.001). In
the perindopril group, 40 deaths (6%) and 22 hospitalizations
(4%) for heart failure occurred, whereas 37 deaths
(6%) and 30 hospitalizations (5%) occurred in the placebo
group. Treatment did not affect death, whereas the
hospitalization rate for heart failure was slightly reduced
(absolute risk reduction, 0.01; 95% confidence interval,
−0.01 to 0.02). No treatment effect on other secondary
end points was detected.
Conclusion:Wefound that 1-year treatment with 8mg/d
of perindopril reduces progressive left ventricular remodeling
that can occur even in the presence of small
infarct size, but it was not associated with better clinical
outcomes