18 research outputs found

    Complete Genome Sequence of Bifidobacterium longum W11 (LMG P-21586), Used as a Probiotic Strain

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    We report the complete genome sequence of Bifidobacterium longum W11 (LMG P-21586) isolated from the intestinal microbiota of a healthy man. The analysis of the sequence may provide insights into the microbiological characteristics and the functional activity of this probiotic strain

    Human natriuretic factor in cirrhotic patients undergoing orthotopic liver transplantation.

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    We measured the plasma levels of atrial natriuretic factor (ANF) during orthotopic liver transplantation (OLT) in eight adult patients with cirrhosis and ascites. The aim of this study was to determine whether significant differences in ANF concentration may be detected during the individual phases of OLT and to correlate these changes with hemodynamics. In each patient a hemodynamic assessment was achieved using a Swan-Ganz fiber optic catheter for continuous monitoring of cardiac output (CO), systemic vascular resistance index (SVRI), right filling pressure as assessed by central venous pressure (CVP), and left filling pressure by means of pulmonary arterial wedge pressure (PAWP). During reperfusion a clear-cut increase in ANF values was observed (P < 0.05). Concurrently, an increase in CVP (P < 0.05) and a decrease in SVRI were observed without any significant increase in diuresis. These data suggest that ANF might play a role in the development of the reperfusion syndrom

    Unusual Tissues and Sample Collection Strategies on Exhumed Bodies

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    The choice of soft or hard tissues to be sampled in case of exhumation of corpses for identification purposes or family relationship testing is based on the degradation conditions of the corpse: the more the corpse is degraded, the less DNA is expected to be retrieved from soft tissue. Therefore, the choice of the \u201cbest\u201d tissue samples usually falls on teeth and bones in these \u201cdifficult\u201d cases, even though the DNA extraction procedure requires time and effort and it can often result in unexpected, negative results. We here present the results of a daily practice survey that shows that it is possible to obtain good results even on DNA extracted from tissues that appear to be less \u201cappealing\u201d to the examiner by performing \u201csimple\u201d corneal/scleral swabs along with cartilage. While DNA extracted from cartilage has been already described, to our knowledge there is no evidence of publications in the scientific literature dealing with cornea/sclera as a source of DNA in the forensic laboratory. The obtained results demonstrate that it may be advisable to consider other tissues which bear the potential of returning good profile results despite not appearing particularly useful and better control of contamination

    Y-STR typing of an Albanian population sample using a 17-loci multiplex PCR system

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    Over the last decade, the analysis of Y-chromosomal STRs has emerged as a powerful tool for paternity testing and forensic casework. More recently, single multiplex PCR assays including new Y-STRs, which complement those comprised in the Y Haplotype Reference Database (YHRD) minimal haplotype (minHt) and the Scientific Working Group on DNA Analysis Methods (SWGDAM) recommended haplotype, have been validated for forensic casework and made commercially available. However, due to the peculiar pattern of inheritance of the Y chromosome, a precise knowledge of the distribution of these extended haplotypes in human populations is required in order to obtain reliable frequency estimates for quantitative assessment of observed matches in kinship analysis and forensic studies. Following a large flow of immigrants from neighbouring Albania during the 1990\u2019s, both paternity and criminal cases involving Albanian individuals have lately became a common occurrence in Italian forensic laboratories. Because of past political isolation, population genetics data from this area of Europe have been historically scarce. As a consequence, there is an almost complete lack of information as to the distribution of DNA polymorphisms of forensic interest in Albanians. Bearing this in mind, a population sample of 108 unrelated first generation male Albanian immigrants residing in Italy was typed using the AmpFlSTR Yfiler PCR amplification kit (Applied Biosystems). This multiplex PCR system includes, apart from the minHt loci, the markers DYS456, DYS458, DYS439, DYS635, Y GATA H4, DYS437, DYS438 and DYS448. A total of 95 different 17-loci Y-STR haplotypes were observed in the Albanian population sample and 86 of them were unique. The commonest haplotype was shared by five individuals. A haplotype diversity of 0.996 and a discrimination capacity of 0.880 were calculated. Among the additional markers, gene diversity ranged between 0.743 (DYS458) and 0.549 (DYS439). Significant differences in allelic distribution and gene diversity were observed within single supplementary Y-STR markers when comparing groups of individuals belonging to distinct Y-SNP haplogroups
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