Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype

We have already characterized Y7 idiotype as natural idiotype by showing its expression on IgM molecules derived form cord sera. The aim of this study was to investigate the binding properties of two human monoclonal IgM antibodies isolated from the sera of patients with Waldenstrom macroglobulinaemia, which express Y7 idiotype. In order to estimate the binding repertoire of these two IgM molecules (IgM DJ and IgM RD) we tested the isolated IgM molecules and their F(ab)2 and Fab fragments over the panel of 23 different protein and non-protein antigens, nine different bacterial strains and cell membranes from neuronal and epithelial tissues. These tests were performed by means of direct and competitive ELISA, immunoblot and immunofluorescence assays. Both antibodies reacted with ssDNA, oligonucleotide fragments, and lactobacteria. IgM DJ showed reactivity with myelin associated glycoprotein (MAG) and blood vessel endothelial cells. Binding for DNA oligonucleotide fragments and lactobacteria was interrupted up to 70% with 0.5 M NaCl, which indicated that these interactions were of low affinity. Avidity dependent binding was also evidenced by use of F(ab)2 fragments. Determined characteristics such as polyreactivity which included autoantigens, low affinity and avidity dependent binding, qualified these two monoclonal IgM as natural autoantibodies. Expression of natural idiotype Y7 on two immunoglobulins M,which possessed natural autoantibody properties, indicated the connection of natural autoantibody specificity and the natural idiotype expression and suggested the involvement of network interactions in selection of malignant B cell clones

Expression of Y7 cross-reactive idiotope on human IgM molecules

In this paper we report data regarding the IgM Y7 cross-reactive idiotope (CRIo) obtained by analysis of: 1) its V-gene subgroup dependance, 2) the frequency of its expression on human monoclonal IgMs and IgM molecules from normal and pathological sera. Furthermore, comparison of epitopic repertoire and nature of binding of human monoclonal IgMs expressing Y7 CRIo was performed to confirm the natural antibody properties of these molecules. IgM isolated from sera of patient DJ (IgM DJ) which expresses the Y7 idiotope has been classified to V(H)3/V(L)2 subgroup. From ten IgMs tested only IgM from patient RD (IgM RD) has been shown to express Y7 idiotope. Y7(+) human IgMs bound to ssDNA, lactic acid bacteria, mouse laminin, porcine thyroglobulin and mouse IgG. Higher percentage of the expression of Y7 CRIo was detected in the sera of patients suffering from autoimmune diseases such as lupus, rheumatoid arthritis and psoriasis vulgaris as well as in patients suffering from chronic infections of the lower urinary tract. Antigen binding repertoire and properties of Y7(+) monoclonal IgM, frequency of Y7 expression on monoclonal IgMs and its concentration in normal and pathological sera indicate the important biological role of this CRIo within the immune system

Difference between Luminal A and Luminal B subtypes according to Ki-67, tumor size, and progesterone receptor negativity providing prognostic information

© the authors, publisher and licensee Libertas Academica Limited. Background: The St. Gallen International Expert Consensus of 2011 proposes a new classification system for breast cancer based on its division into five subgroups. The criteria to identify these subtypes were recently refined at the 2013 Conference. In this respect, the authors of this paper have conducted a retrospective analysis of breast cancer subtypes, related to Ki-67 and involvement of the axillary lymph nodes (ALNs). The analysis was performed only in the cases of invasive breast cancer in the pT2 stages. The research and results of the paper have shown that investigating the value of these parameters could be of great benefit in future treatment strategies of invasive breast cancer.Results: The conclusion was made that when Ki-67 was found to be higher, patients also showed a higher involvement in their ALNs. The chi-square test shows the difference to be significant (chi-square = 4.757; P = 0.029). Luminal B subtypes had the highest percentage (54.9%) of involvement of lymph nodes when compared to the other four subtypes. The Luminal B subtype had a higher percentage (51.4%) of involvement of lymph nodes than did Luminal A (10.7%). The chi-square test also shows the difference to be significant (P, 0.05).Methods: A retrospective analysis of breast cancer subtypes, tumor nodal metastatic staging, and histopathological grading of 108 cases has been performed according to the methods recommended and provided by the St. Gallen International Expert Consensus Report, 2011. The estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER2), and Ki-67 of 108 tumor samples were all investigated by immu-nohistochemistry according to the methods used to classify breast cancer subtypes as proposed in the St. Gallen Consensus Report, 2011. Invasive breast cancers (n = 108) were immunohistochemically classified as follows: 28 (25.92%) as Luminal A, 51 (47.22%) as Luminal B (HER2 negative), 21 (19.44%) as Luminal B-like (HER2 negative), 2 (1.85%) as HER2 positive, and 6 (5.55%) as being a triple-negative subtype.Conclusion: A combination of the Ki-67 index, HER negative tumors, PR negativity, and a low value that can be used to segregate ER positive pT2 tumors into prognostically significantly different clinical outcomes may be utilized clinically to guide patient management in accordance with these tumor characteristics

JSCS–3827 Original scientific paper

The monoclonal antibody 26 raised against tetanus toxoid also recognizes tetanus toxin and β2-glycoprotein I – its binding properties in vitro and potential application

Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246

Supporting information for: [https://doi.org/10.1371/journal.pone.017246]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2417

Poor life habits: Risk factors for formation osteoporosis

The use of bone density measurements is of central importance for diagnosis, prognosis end the assessment of treatment of patients with osteoorosis. Risk factors are variables which significantly change expected absolute risk, that is, the probability of variable disease in a certain population. Aging and smoking are the most common listed risk factors for the development of osteoporosis. The aim of our work was to confirm the most common risk factors for osteoporosis. In our research work has processed a total of 88 smokers were divided into groups according to the number of cigarettes smoked and the length of smokers. Results showed that risk factors: poor life habits (smoking and use of coffee), are significantly represented on patients with osteoporosis. In order to timely implement treatment, we concluded based on the results, that there is a need actively look for presence of risk factors responsible for osteoporosis in our patients

Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246

Supporting information for: [https://doi.org/10.1371/journal.pone.017246]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2417