Tentative phylogenetic affiliation of clones derived from bands excised from bacterial DGGE fingerprints in Figure 3.

<p>GenBank 14-March-2014.</p><p>Tentative phylogenetic affiliation of clones derived from bands excised from bacterial DGGE fingerprints in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106865#pone-0106865-g003" target="_blank">Figure 3</a>.</p

Bacterial communities of long-term matured artificial soils.

<p>a) Total bacterial DGGE fingerprints of unspiked control artificial soils (four replicates) sampled on the day of spiking. Arrows mark soil composition-specific populations in QM (bands A), QMC (B), QI (C), and QIF (D). BS-bacterial DGGE standard. Ino-Luvisol inoculant added to soil mixtures at the beginning of incubation. Q-quartz, M-montmorillonite, C-charcoal, I-illite, F-ferrihydrite. b) Corresponding UPGMA cluster analysis.</p

Response of bacterial communities to spiking in QM and QMC soils.

<p>DGGE fingerprints of bacterial communities in spiked QM and QMC soils sampled 21 days after spiking (control, phenanthrene (+P), litter (+L), litter and phenanthrene [+L+P]). Arrows mark populations responding to litter (black), phenanthrene (white), litter and phenanthrene (grey). Numbers indicate excised and cloned bands (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106865#pone-0106865-t002" target="_blank">Table 2</a>). BS-bacterial DGGE standard. Ino-Luvisol inoculant added to soil mixtures at the beginning of incubation. QM/QMC ctr-bacterial community in QM or QMC, respectively, before spiking. Q-quartz, M-montmorillonite, C-charcoal.</p

Experimental design.

<p>Long-term matured artificial soils QM, QMC, QI, QIF and the natural soil were subjected to following treatments: unspiked control, phenanthrene (+P), plant litter (+L), litter and phenanthrene (+L+P). Each treatment consisted of four replicates. Sampling was carried out on the day of spiking, 7, 21 and 63 days after spiking (DAS). Q-quartz, M-montmorillonite, C-charcoal, I-illite, F-ferrihydrite, Luv-natural soil (Luvisol).</p

Response of fungal communities to spiking in QI and QIF soils.

<p>DGGE fingerprints of fungal communities in spiked QI and QIF soils sampled 63 days after spiking (control, phenanthrene (+P), litter (+L), litter and phenanthrene [+L+P]). Arrows mark populations responding to litter (black), litter and phenanthrene (grey). FS-fungal DGGE standard. QI/QIF ctr-fungal community in QI or QIF, respectively, before spiking. Q-quartz, I-illite, F-ferrihydrite.</p

Percent difference (d-values) between microbial communities of different spiking treatments for different taxa and different sampling times (21 and 63 days after spiking [DAS]) per soil.

<p>D-values were calculated based on pairwise Pearson similarity coefficients for the effect of phenanthrene (+P) in non-litter or litter-amended soils and the effect of litter (+L) in the absence or presence of phenanthrene. Bold numbers indicate significant differences (p<0.05). Q-quartz, M-montmorillonite, C-charcoal, I-illite, F-ferrihydrite, Luv-natural soil (Luvisol).</p><p>Percent difference (d-values) between microbial communities of different spiking treatments for different taxa and different sampling times (21 and 63 days after spiking [DAS]) per soil.</p

Quantification of 16S rRNA gene and ITS fragments in spiked soils.

<p>The qPCR analysis of (a) bacterial 16S rRNA gene copy numbers in samples taken 21 days after spiking (DAS) and (b) of fungal ITS fragment copy numbers in samples taken 63 DAS in the following treatments: unspiked (control), phenanthrene (+P), litter (+L), litter and phenanthrene (+L+P). Different letters within one soil composition mark a significant difference between treatments (Tukey test, p<0.05). Bars indicate standard deviation of four replicates. Q-quartz, M-montmorillonite, C-charcoal, I-illite, F-ferrihydrite, Luv-natural soil (Luvisol).</p

Phenanthrene concentrations in mg g⁻¹ soil determined in three replicates per soil composition in the phenanthrene (+P), litter and phenanthrene (+L+P) treatments on the day of spiking and 21 days after spiking (DAS).

<p>Q-quartz, M-montmorillonite, C-charcoal, I-Illite, F-ferrihydrite, Luv-natural soil (Luvisol).</p><p>Phenanthrene concentrations in mg g⁻¹ soil determined in three replicates per soil composition in the phenanthrene (+P), litter and phenanthrene (+L+P) treatments on the day of spiking and 21 days after spiking (DAS).</p

Giese et al 2013_suppl_meteorological data IMGERS 2004-2006

daily meteorological data of the experimental years 2004 -200

Soil temperature differences (5 cm depth) between beech-soil-mesocosms incubated at SW exposure (warm-dry microclimate, climate change treatment) and at NW exposure (cool-moist microclimate, control treatment).

<p>Data represent mean values of five temperature probes per treatment directly installed horizontally in soil of transferred beech-soil-mesocosms. Arrows indicate the three sampling campaigns. The period between the sampling in June and August equals the roof period of 39 days.</p