4 research outputs found

    Nutrient Content of Napier Grass (Pennisetum Purpureum) Silage Made with Various Additive and Modified Atmosphere in the Silo

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    . During ensilage, anaerob condition must be controlled. Some methods of modified atmosphere in silo were analyzed to compare ensilage characteristics and silage product. So far, there is not been information on the atmosphere condition in the process of silage production. It encourages the researchers to evaluate the condition of ensilage process of Pennisetum purpureum by studying atmosphere modification in the silo and the effect of the USAge of various additives in the process of silage production. Elephant grass (Pennisetum purpureum), molasses, L. acidophillus were used. The study was conducted with a Completely Randomized Design (CRD) 3x2 factorial pattern. Atmosphere modification as the first factor consist of : (A0: silage with compaction (conventional) A1: silage with vacuum method, A2: silage with modified C02) and two kinds of silage additives as the second factor (B1: indirect additive (molasses); B2: direct additive (Lactic Acid Bacteria). Each treatment combination was repeated 4 times. The objective of the research was to evaluate changes in nutrient content (protein, crude fiber, gross energy). The data obtained were analyzed by analysis of variance, then continued by Honest Significant Differences (HSD) test. Based on the research results it can be concluded that the optimum ensilage can take place, either by compaction methods (conventional), vacuum and the addition of CO2. While the addition of molasses additive produces silage with better quality than the addition of L. Acidophillus inoculant

    The Efficacies of Banana Stem Extract as a Candidate of Coccidiostat Against Rabbit Eimeria Stiedaio Ocysts: an in Vitro Analysis

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    The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of different concentration from banana stem extract 0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation

    Body Weight, Oocyte Elimination and Blood Profile of Rabbit After Challenge Test Using Eimeria Stiedai

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    The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbits' blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbits' body immune against coccidiosis infection
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