Sarkoid donjeg očnog kapka kod magarca (Equus asinus) izazvan bovinim papiloma 2 virusom

Sarcoid tumors were described by means of histopathological and molecular procedures in a 5-year-old donkey. Histopathological examination showed epithelial changes including hyperkeratosis, epithelial hyperplasia, koilocytosis, and rete peg formation. Neoplastic fibroblastic cells were plumb, large spindle to stellate and embedded in dense collagenous tissue. Results of Polymerase Chain Reaction and DNA sequence analysis showed that the etiological agent belonged to Bovine Papilloma Virus-II species in the delta papilloma virus genus. This case study represents the first report demonstrating the presence of Bovine Papilloma Virus-II in donkey sarcoid.Sarkoidi su opisani primenom histopatoloških i molelularanih metoda i procedura kod magarca, starog pet godina. Histopatološko ispitivanje, ukazalo je na promene u epitelu koje su uključivale hiperkeratozu, hiperplaziju epitela, koilocitozu i formiranje invaginacija epitela ptema dubokom dermisu - rete peg. Fibroblastine neoplastične ćelije su bile krupne, ovalnovretenaste i zvezdaste, uronjene u tkivo bogato kolagenom. Ispitivanje PCR metodom kao i rezultati DNK sekvencioniranja pokazali su da je etiološki agens spadao u grupu bovinog papilomavirusa-2 koji pripada rodu delta papilomavirusa. Ova studija predstavlja prvi opisani slučaj prisustva papiloma virusa goveda-2 kod sarkoida magarca

Koinfekcija teladi virusom papularnog stomatitisa goveda, rotavirusom i Cryptosporidium Spp

Concurrent occurence of bovine papular stomatitis, rotavirus infection and cryptosporidiosis was diagnosed postmortem in a 7-days-old calf from a farm containing 65 calves of different ages. Multifocal papular stomatitis and rumenitis were present on necropsy. While polymerase chain reaction analysis revealed rotavirus and papular stomatitis virus infections; bovine viral diarrhea, foot and mouth disease, bovine papilloma virus and coronavirus could not be detected. Overall; concurrent co-infection with bovine papular stomatitis virus, rotavirus and cryptosporidium spp. was reported for the first time.Postmortalno je dijagnostikovana infekcija virusom papuloznog stomatitisa, rotavirusna infekcija kao i kriptosporidioza kod teleta starog 7 dana, na farmi sa 65 teladi različitog uzrasta. Prilikom obdukcije, uočene su multifokalne lezije karakteristične za papulozni stomatitis kao i rumenitis. Testom lančane reakcije polimeraze (PCR) dokazano je prisustvo rotavirusa i virusa izazivača papuloznog stomatitisa, pri čemu nisu dokazani virusi izazivači bovine virusne dijareje, slinavke i šapa, papilomatoze i koronavirusne infekcije. Uopšteno, ovim je po prvi put je prikazan slučaj koinfekcije virusom izazivačem papuloznog stomatitisa, rotavirusne infekcije i infekcija sa Cryptosporidium spp

Sarcoid in the Lower Eyelid Due to Bovine Papillomavirus-2 in a Donkey ( Equus Asinus

Sarcoid tumors were described by means of histopathological and molecular procedures in a 5-year-old donkey. Histopathological examination showed epithelial changes including hyperkeratosis, epithelial hyperplasia, koilocytosis, and rete peg formation. Neoplastic fibroblastic cells were plumb, large spindle to stellate and embedded in dense collagenous tissue. Results of Polymerase Chain Reaction and DNA sequence analysis showed that the etiological agent belonged to Bovine Papilloma Virus-II species in the delta papilloma virus genus. This case study represents the first report demonstrating the presence of Bovine Papilloma Virus-II in donkey sarcoid.Sarkoidi su opisani primenom histopatoloških i molelularanih metoda i procedura kod magarca, starog pet godina. Histopatološko ispitivanje, ukazalo je na promene u epitelu koje su uključivale hiperkeratozu, hiperplaziju epitela, koilocitozu i formiranje invaginacija epitela ptema dubokom dermisu - rete peg. Fibroblastine neoplastične ćelije su bile krupne, ovalnovretenaste i zvezdaste, uronjene u tkivo bogato kolagenom. Ispitivanje PCR metodom kao i rezultati DNK sekvencioniranja pokazali su da je etiološki agens spadao u grupu bovinog papilomavirusa-2 koji pripada rodu delta papilomavirusa. Ova studija predstavlja prvi opisani slučaj prisustva papiloma virusa goveda-2 kod sarkoida magarca

Case report: systemic tuberculosis caused by Mycobacterium bovis in a cat

Abstract Background The diagnosis of previous cases of feline tuberculosis in Turkey has been made based solely on pathological changes without isolation of the causative agent. This case report details the first case of feline tuberculosis in Turkey for which the causative agent (Mycobacterium bovis) was confirmed with microbiological isolation, morphological evaluation, molecular (PCR) characterization and antibiotic sensitivity. Case presentation Systemic tuberculosis was diagnosed via postmortem examination of a 5-year-old stray male cat. Mycobacterium bovis was isolated from the lungs, bronchial and gastrointestinal lymph nodes, kidney and liver. The isolate was defined as M. bovis using the Genotype MTBC assay (Hain Lifescience, Germany), which allows differentiation of species within the Mycobacterium tuberculosis complex with an easy-to-perform reverse hybridization assay. Pathological changes were characterized by multifocal to coalescing granulomatous inflammation in the lungs, liver, lymph nodes and kidneys. Further pathological changes included severe, diffuse, hepatocytic atrophy, periportal fibrosis with lymphohistiocytic infiltration, multifocal lymphohistiocytic interstitial nephritis, mild focal pulmonary anthracosis and mild renal and hepatic amyloidosis. Infection by immunosuppressive viral pathogens including feline herpes virus-1, feline immunodeficiency virus and feline parvovirus virus were ruled out by polymerase chain reaction assay (PCR). The isolated mycobacteria were susceptible to isoniazid, ethambutol, rifampicin or streptomycin. Conclusion Disseminated M. bovis is a rare infection in cats. Involvement of submandibular lymph nodes suggested that primary transmission might have been the oral route in the present case

Detection and molecular characterization of Clostridium perfringens, Paeniclostridium sordellii and Clostridium septicum from lambs and goat kids with hemorrhagic abomasitis in Turkey

Abstract Background The pathogenic Clostridia cause neurotoxic, histotoxic and enterotoxic infections in humans and animals. Several Clostridium species have been associated with abomasitis in ruminants. The present study aimed to investigate the frequency, and the presence of virulence genes, of Clostridium perfringens, Paeniclostridium sordellii and Clostridium septicum in lambs and goat kids with hemorrhagic abomasitis. Results A total of 38 abomasum samples, collected from lambs and goat kids of 1 week to 1 month of age in different farms located in eastern Turkey between 2021 and 2022, were evaluated by histopathology, culture and PCR. At necropsy, the abomasum of the animals was excessively filled with caseinized content and gas, and the abomasum mucosa was hemorrhagic in varying degrees. In histopathological evaluation, acute necrotizing hemorrhagic inflammation was noted in abomasum samples. The examination of swab samples by culture and PCR revealed that C. perfringens type A was the most frequently detected species (86.84%) either alone or in combination with other Clostridium species. P. sordellii, C. perfringens type F and C. septicum were also harboured in the samples, albeit at low rates. Beta2 toxin gene (cpb2) was found in three of C. perfringens type A positive samples. Conclusion It was suggested that vaccination of pregnant animals with toxoid vaccines would be beneficial in terms of protecting newborn animals against Clostridial infections. This study investigated the presence of clostridial toxin genes in abomasal samples for the first time in Turkey