Leukocytes Differentiation of Pangasius Hypopthalmus That Were Feed with Curcumin Extract From Curcuma Domestica V

This research was conducted on June until August 2016 in Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine science, University of Riau, Pekanbaru. The aim of this research was to determine leucocyte differentiation of Pangasius hypopthalmus that were feed with curcumin extract from Curcuma domestica V. This research used five treatment such as; Kn (negative control): that were feed without curcumin extract and were not infected with A. hydrophila, Kp (positive control): that were feed without curcumin extract and infected with A. hydrophila, P1: feed with curcumin extract in 0,3g/kg, P2: feed with curcumin extract in 0,5g/kg and P3: feed with curcumin extract in 0,7 g/kg. Fish reared in an aquarium the size of 40x30x30 cm with stocking density 10 fish / aquarium, feeding as many as 10% of body weight and do 3 times a day. Pisciculture carried out for 30 days and after that fish infected with Aeromonas hydrophila. The results shown that P3 (feed with curcumin extract in 0,7 g/kg) were the best treatment with lymphocytes 83,00%; monocytes 5.33%; neutrophils 3,67%; thrombocyte 8.00%; total leukocytes 12,01x104 cell/mm3; and fagocyte index 58,33

Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

Background Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)