Development of a Rapid Molecular Test Format Based on Isothermal Recombinase Polymerase Amplification Technique and Lateral Flow Method for Detection of bla(OXA-48)

Objective: Molecular tests are rapid, reliable tools for the detection of carbapenem resistance, but their use is limited due to their cost, requirement for well-trained technicians and highly sophisticated instrumentation. The recombinase polymerase amplification (RPA) assay, one of the isothermal amplification methods developed recently to overcome these problems, doesn't require denaturation of target, and RPA products can be determined by probe-base detection methods even without a specific instrumentation. In this study, we aimed to develop a rapid and easily applicable molecular test format in on-site settings based on RPA technique with combination of lateral flow system for detection of bla(OXA-48)

Polymorphism of hypoxia-inducible factor-1 α gene in pediatric acute respiratory distress syndrome

Objective: To examine if hypoxia-inducible factor-1 α (Hif-1 α ) polymorphisms are associated with pediatric acute respiratory distress syndrome (PARDS). Methods: Twenty two patients with PARDS and 11 non-PARDS controls were examined in pediatric intensive care unit in Cukurova University Balcali Hospital. Blood polymorphism was used to assess the Hif-1 α C1772T and G1790A polymorphisms of Hif-1 α gene, and differences in genotypes between the 2 groups were compared. Results: Hif-1 α C1772T polymorphism was observed only in one case of PARDS group but non-PARDS group didn’t show any C1772T polymorphism. Particularly, the difference in number of cases with Hif-1 α G1790A polymorphism was not significant between PARDS and non-PARDS groups. In addition, Hif-1 α G1790A polymorphism was significantly related to the distribution of lung opacities in children with PARDS (P<0.05). Conclusions: Our results indicate that Hif-1 α G1790A polymorphism is related to an increased susceptibility to pulmonary for PARDS children. The detection of G1790A polymorphism could help pediatricians to predict the extensity of PARDS early in lung tissue

Evaluation of Adenovirus-36 (Ad-36) antibody seropositivity and adipokine levels in obese children

Adenovirus 36 (Ad-36) has recently been suggested as a possible contributor to the current obesity epidemic. The aim of this study was to investigate the prevalence of Ad-36 antibodies in obese children, as well as investigate the role of serum leptin and lipid levels in Ad-36-obesity. Seventy-one obese children and 62 non-obese children were included as the patient group (PG), including the healthy control group (HCG), respectively. Simultaneously, Ad-36 antibodies and adipokine levels were assessed with serum neutralization assays (SNA) and ELISA. Ad-36 antibody was detected in 9 patients (12.7%) and 1 patient (1.6%) in both the PG and HCG, respectively, while a significant difference was detected between groups (p < 0.05). Although serum LDL, total cholesterol, triglycerides and leptin levels were detected significantly higher, adiponectin level was detected paradoxically lower in the PG. However, a significant difference was not detected for lipids and leptin levels; adiponectin levels were found to be significantly lower in Ad-36 antibody-positive PG (p < 0.05)