Mouse prostate strain-associated gene expression and analysis in human prostate tissues: FVB/N and C57BL/6

Genes differentially expressed in prostates of FVB/N and C57BL/6 strains. Heat map colors reflect fold ratio values between sample and reference pool. Columns 1-4 represent biological replicates for each strain. Rows represent individual genes. Values shown in red are relatively larger than the overall mean; values shown in green are relatively smaller than the overall mean. Transcript abundance levels in benign human prostate tissues associated with high grade (7-10) or low grade (≤6) adenocarcinomas for each gene determined to be altered in mouse strain comparisons where a corresponding ortholog was identified. Genes depected in (a) and (b) are in identical order. Black box (b) and text (a) represent genes with significant differential expression in the human datasets altered in the expected orientation. Gray box (b) and text (a) represent genes with significant differential expression in the human datasets altered in the opposite orientation. Transcript alterations for selected genes in benign tissue samples associating with high (Gleason 7-10) and low (Gleason ≤6) prostate cancers. Plots represent the 95% confidence intervals of logexpression ratios of tissues samples relative to a cell line reference.<p><b>Copyright information:</b></p><p>Taken from "Genetic background influences murine prostate gene expression: implications for cancer phenotypes"</p><p>http://genomebiology.com/2007/8/6/R117</p><p>Genome Biology 2007;8(6):R117-R117.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC2394769.</p><p></p

Analysis of strain-dependent differences in prostate gene expression by qRT-PCR

RNAs from preparations used in the microarray analysis or microdissected epithelium were reverse transcribed and amplified using qRT-PCR with primers specific for (), (), () and (). Ribosomal protein S16 expression levels were used to normalize qRT-PCR data. Normalized results are expressed relative to the lowest expressing value. Error bars indicate the standard deviation of four biological independent replicates. qRT-PCR for microdissected epithelium is represented by one sample per strain for each gene. White bars denote measurements from the microarray analysis. Black bars denote measurements generated by qRT-PCR from whole prostate. Diagonal lines denote measurements generated by qRT-PCR from microdissected prostate epithelium.<p><b>Copyright information:</b></p><p>Taken from "Genetic background influences murine prostate gene expression: implications for cancer phenotypes"</p><p>http://genomebiology.com/2007/8/6/R117</p><p>Genome Biology 2007;8(6):R117-R117.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC2394769.</p><p></p

Mouse prostate strain-associated gene expression and analysis in human prostate tissues: BALB/c and C57BL/6

Genes differentially expressed in prostates of BALB/c (BALB) and C57BL/6 (C57) strains. Heat map colors reflect fold ratio values between sample and reference pool. Columns 1-4 represent biological replicates for each strain. Rows represent individual genes. Values shown in red are relatively larger than the overall mean; values shown in green are relatively smaller than the overall mean. Transcript abundance levels in benign human prostate tissues associated with high grade (7-10) or low grade (≤6) adenocarcinomas for each gene determined to be altered in mouse strain comparisons where a corresponding ortholog was identified. Genes depicted in (a) and (b) are in identical order. Black box (b) and text (a) represent genes with significant differential expression in the human datasets altered in the expected orientation. Gray box (b) and text (a) represent genes with significant differential expression in the human datasets altered in the opposite orientation. Transcript alterations for selected genes in benign tissue samples associating with high (Gleason 7-10) and low (Gleason ≤6) prostate cancers. Plots represent the 95% confidence intervals of logexpression ratios of tissues samples relative to a cell line reference.<p><b>Copyright information:</b></p><p>Taken from "Genetic background influences murine prostate gene expression: implications for cancer phenotypes"</p><p>http://genomebiology.com/2007/8/6/R117</p><p>Genome Biology 2007;8(6):R117-R117.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC2394769.</p><p></p