RNAs from preparations used in the microarray analysis or microdissected epithelium were reverse transcribed and amplified using qRT-PCR with primers specific for (), (), () and (). Ribosomal protein S16 expression levels were used to normalize qRT-PCR data. Normalized results are expressed relative to the lowest expressing value. Error bars indicate the standard deviation of four biological independent replicates. qRT-PCR for microdissected epithelium is represented by one sample per strain for each gene. White bars denote measurements from the microarray analysis. Black bars denote measurements generated by qRT-PCR from whole prostate. Diagonal lines denote measurements generated by qRT-PCR from microdissected prostate epithelium.<p><b>Copyright information:</b></p><p>Taken from "Genetic background influences murine prostate gene expression: implications for cancer phenotypes"</p><p>http://genomebiology.com/2007/8/6/R117</p><p>Genome Biology 2007;8(6):R117-R117.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC2394769.</p><p></p
