The Use of AlphaScreen Technology in HTS: Current Status

AlphaScreen (Amplified Luminescent Proximity Homogeneous Assay Screen) is versatile assay technology developed to measuring analytes using a homogenous protocol. This technology is an example of a bead-based proximity assay and was developed from a diagnostic assay technology known as LOCI (Luminescent Oxygen Channeling Assay). Here, singlet oxygen molecules, generated by high energy irradiation of Donor beads, travel over a constrained distance (approx. 200 nm) to Acceptor beads. This results in excitation of a cascading series of chemical reactions, ultimately causing generation of a chemiluminescent signal

Structures et mecanismes impliques dans l'assemblage et l'activation du complexe C1 de la voie classique du systeme complementaire

SIGLEAvailable from INIST (FR), Document Supply Service, under shelf-number : T 84800 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Role of the occluding loop in cathepsin B activity

UI - 97150885NRC publication: Ye