Продукција антибиотика помоћу Streptomyces hygroscopicus CH-7 у хранљивим подлогама са комплексима шифових базa

Influence of a modified media on Hexaene H-85 and Azalomycine B production by Streptomyces hygroscopicus CH-7 was investigated. The amino acid L-tryptophan, as a nitrogen source, was replaced with isatin-3-thiosemicarbazone and its complexes with some divalent metal ions. Isatin-3-thiosemicarbazone was synthesized in crude glycerol obtained as a byproduct in biodiesel production from sunflower oil. The complexes were charac-terized by elemental microanalysis and magnetic susceptibility, as well as, by Atomic absorption(AA), Fourier-transform infrared (FTIR) and Ultraviolet–visible (UV/VIS) spectro-scopic methods. The spectral studies indicated an octahedral geometry for the Mn(II), Fe(II) and Ni(II) complexes and a tetrahedral one for the Zn(II) complex. Comparing to the basal medium, isatin-3-thiosemicarbazone (ITC) and its metal complexes in the concentration of 0.5 g dm-3 showed better results in the antibiotics production. Use of medium supple-mented with the Fe(II) complex resulted in the maximum Hexaene H-85 and Azalomycine B concentrations of 306 μg cm-3 and 127 μg cm-3, respectively. Addition of ITC and its complexes changed the morphology of S. hygroscopicus CH-7 from filaments to pellets as a dominant shape in media resulting in higher antibiotic production.У раду је испитиван утицај модификованих хранљивих подлога на производњу антибиотика Hexaene H-85 и Azalomycine B помоћу Streptomyces hygroscopicus CH-7. Аминокиселина L-триптофан, као извор азота, замењена је изатин-3-тиосемикарбазоном (синтетисаним у сировом глицеролу који се добија као нуспродукт у производњи биодизела из сунцокретовог уља) и његовим комплексима са неким двоалентним металним јонима. Комплекси су карактерисани елементарном микроанализом, магнетном сусцептибилнош-ћу, атомском аспорпцијом (A), инфрацрвеним (IR) и ултравиолетним (UV/VIS) спектроскопским методама. Спектралне анализе показале су октахедралну геометрију комплекса Мn(II), Fе(II) и Ni(II) и тетраедарску за комплекс Zn(II). Изатин-3-тиосемикарбазон и његови комплекси са металима (0,5 g dm-3) утичу на повећање продукције антибиотика у поређењу са основном подлогом. Највеће концентрације антибиотика Hexaene H-85 и Azalomycine B су остварене у хранљивој подлози са Fe(II) комплексом (306 и 127 μg cm-3, редом). Комплекси ИТС и изатин 3-тиосемикарбазон утичу и на морфологију тестираног соја. Streptomyces hygroscopicus CH-7 растe у облику филамената и пелета, у подлогама у којима је остварена највећа продукција антибиотика

Lipoprotein(a) Is the Best Single Marker in Assessing Unstable Angina Pectoris

This study evaluated whether statin therapy changed a diagnostic validity of lipid and inflammatory markers in ischemic heart disease (IHD) patients. Levels of lipids, lipoproteins, apolipoproteins, inflammatory markers, and atherogenic indexes were determined in 49 apparently healthy men and women, 82 patients having stable angina pectoris (SAP), 80 patients with unstable angina (USAP), and 106 patients with acute ST-elevation myocardial infarction (STEMI) treated or not treated with statins. Diagnostic accuracy of markers was determined by ROC curve analysis. Significantly lower apoA-I in all statin-treated groups and significantly higher apoB in statin-treated STEMI group compared to non-statin-treated groups were observed. CRP showed the best ROC characteristics in the assessment of STEMI patients. Lp(a) is better in the evaluation of SAP and USAP patients, considering that Lp(a) showed the highest area under the curve (AUC). Regarding atherogenic indexes, the highest AUC in SAP group was obtained for TG/apoB and in USAP and STEMI patients for TG/HDL-c. Statins lowered total cholesterol, LDL-c, and TG but fail to normalize apoA-I in patients with IHD

The impact of Schiff bases on antibiotic production by Streptomyces hygroscopicus

A media consisting of isatin-Schiff bases (isatin-3-thiosemicarbazone, isatin-3-semicarbazone, and isatin-3-phenylhydrazone) was developed to maximize the production of antibiotics Hexaene H-85 and Azalomycine B by Streptomyces hygroscopicus. The media isatin-3-thiosemicarbazone resulted in the maximum antibiotics concentration of 372 μg cm-3 for Hexaene H-85 and 118 μg cm-3 for Azalomycine B. The impact of modified media on soil morphology also was investigated

Sirovi glicerol i surutka kao izvori ugljenika i azota za produkciju antibiotika

The microorganisam Streptomyces hygroscopicus CH-7 produces antibiotics Hexaene, Nigericine and Elaiophyline. The yield of antibiotics can be increased by changing the fermentation conditions and the composition of the nutrient medium. Based on the previous research with this strain and the literature reviewed, the aim of this work was to study the possibility of using waste materials (whey with proteins in varying ammounts and crude glycerol from the biodiesel production) as a carbon and nitrogen source in order to increase the yeald of antibiotics produced by Streptomyces hygroscopicus CH-7. The maximum concentration of Hexaene (47,2 μg/ cm3) was achieved in the medium with whey containing 80% of protein and glycerol from sunflower oil, while the maximum concentration of Elaiophyline (24,3μg/cm3) was achived in the basal medium.Mikroorganizam Streptomyces hygroscopicus CH-7 produkuje antibiotike Heksaen, Nigericin i Elaiofilin. Promenom uslova fermentacije i sastava hranljive podloge može se povećati produkcija antibiotika. Na osnovu dosadašnjih istraživanja sa ovim sojem i literaturnog pregleda, cilj ovog rada je bio da se ispita mogućnost primene otpadnih sirovina (surutke sa različitim procentom proteina i sirovog glicerola iz proizvodnje biodizela) kao izvora ugljenika i azota, kako bi se povećala produkcija antibiotika pomoću Streptomyces hygroscopicus CH-7. Maksimalna koncentracija Heksaena (47,2 μg/cm 3) postignuta je na podlozi sa surutkom (koja sadrži 80% proteina) i glicerolom iz suncokretovog ulja, dok je maksimalna koncentracija Elaiofilina (24,3μg/cm3) ostvarena na osnovnoj podlozi

The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7

In the present work, four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. The obtained compounds were identified by elemental microanalysis, as well as FTIR, UV/Vis and H-1-NMR spectroscopic methods. Yields of 93-98% of the compounds in crude glycerol were achieved within 10-25 min. The derivatives of salicylaldehyde and crude glycerol were used as a nitrogen and carbon source, respectively, in the medium for antibiotic (hexaene H-85 and azalomycine B) production by Streptomyces hygroscopicus CH-7. The highest concentrations of hexaene H-85 and azalomycine B were achieved in the medium containing salicylaldehyde-thiosemicarbazone (198 and 69 mu g/cm(3), respectively). Derivatives of salicylaldehyde also impacted the strain morphology. In the media with salicylaldehyde- phenylhydrazone and salicylaldehyde-thiosemicarbazone, S. hygroscopicus CH-7 grew like large dispersive pellets with long twisted filaments that produced the highest yield of the antibiotics

ANTIMICROBIAL ACTIVITY OF BIOACTIVE COMPONENT FROM FLOWER OF LINUM CAPITATUM KIT

Abstract. Different extracts containing bioactive components and etheric oil of the flowers of Linum capitatum Kit. (Linacea) of Serbian origin were tested for an antimicrobial activity against four bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilus, Pseudomonas aeruginosa), one mold (Aspergillus niger) and one yeast (Candida albicans). The isolated flavonoids were also tested against Staphylococcus aureus, Escherichia coli, Bacillus anhtracis, Pseudomonas aeruginosa, Aspergillus niger, Candida albicans and Herpes simplex virus type

Impact of carboxymethylcellulose on morphology and antibiotic production by Streptomyces hygroscopicus

A chemically defined media consisting of carboxymethylcellulose (CMC) was developed to maximize the production of antibiotics, hexaene H-85 and azalomycine, by Streptomyces hygroscopicus CH-7. The production of antibiotics by filamentous organisms is often dependent on the morphology and size distribution of the pellet population within the culture. By adding the polymer to the fermentation medium, the growth was changed from a single large glob to small reproducible pellets, and wall growth was diminished to a minimum. Maximum concentrations of hexaene H-85 (146.7 mg/dm3) and azalomycine (188.6 mg/dm3) were reached at 3.0% and 1.0% (w/v) CMC, respectively

Valorization of crude glycerol from biodiesel production

The increased production of biodiesel as an alternative fuel involves the simultaneous growth in production of crude glycerol as its main by-product. Therefore, the feasibility and sustainability of biodiesel production requires the effective utilization of crude glycerol. This review describes various uses of crude glycerol as a potential green solvent for chemical reactions, a starting raw material for chemical and biochemical conversions into value-added chemicals, a substrate or co-substrate in microbial fermentations for synthesis of valuable chemicals and production of biogas and biohydrogen as well as a feedstuff for animal feed. A special attention is paid to various uses of crude glycerol in biodiesel production. [Projekat Ministarstva nauke Republike Srbije, br. III 45001

The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7

In the present work, four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. The obtained compounds were identified by elemental microanalysis, as well as FTIR, UV/Vis and 1H-NMR spectroscopic methods. Yields of 93–98% of the compounds in crude glycerol were achieved within 10–25 min. The derivatives of salicylaldehyde and crude glycerol were used as a nitrogen and carbon source, respectively, in the medium for antibiotic (hexaene H-85 and azalomycine B) production by Streptomyces hygroscopicus CH-7. The highest concentrations of hexaene H-85 and azalomycine B were achieved in the medium containing salicylaldehyde-thiosemicarbazone (198 and 69 μg/cm3, respectively). Derivatives of salicylaldehyde also impacted the strain morphology. In the media with salicylaldehyde- phenylhydrazone and salicylaldehyde-thiosemicarbazone, S. hygroscopicus CH-7 grew like large dispersive pellets with long twisted filaments that produced the highest yield of the antibiotics. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 45001