CASE STUDY ON ATTITUDE AND BEHAVIOR OF SCHOOL LUNCH OFFICIALS REGARDING UTILIZATION OF LOCAL PRODUCE -FOCUSING ON THE DIFFERENCE OF OCCUPATION

This study was conducted in January 2010 to identify influential factors for utilization of local produce in school lunch services and factor-correlations in terms of employment types and item types and to review the evaluation index for shokuiku. The research participants were 56 nutrition teachers, 91 school principals, and 23 school lunch directors. The study revealed that school lunch staff had high awareness and behavioral motivation for using local ingredients. School lunch directors were concerned about managerial aspects such as “high price” and “not enough variety and quantity” for making lunches with local ingredients only.  The principals expressed high opinion on educational or administrative advantages of using local ingredients in “increased number of local cuisine in the menu” and “securing food safety” but showed low awareness on problems related to using local produce to cook for entire student body such as “longer cooking time” and “not enough providers.” Correlation between questionnaire items revealed that there was a strong positive relation between items emphasizing advantages of using local produce. The results showed that using local produce for providing school lunches could induce changes in behaviors, such as frequenting local produce markets, and it is considered to become a good evaluation index for developing shokuiku

CASE STUDY ON ATTITUDE AND BEHAVIOR OF SCHOOL LUNCH OFFICIALS REGARDING UTILIZATION OF LOCAL PRODUCE -FOCUSING ON THE DIFFERENCE OF OCCUPATION

This study was conducted in January 2010 to identify influential factors for utilization of local produce in school lunch services and factor-correlations in terms of employment types and item types and to review the evaluation index for shokuiku. The research participants were 56 nutrition teachers, 91 school principals, and 23 school lunch directors. The study revealed that school lunch staff had high awareness and behavioral motivation for using local ingredients.School lunch directors were concerned about managerial aspects such as “high price” and “not enough variety and quantity” for making lunches with local ingredients only. The principals expressed high opinion on educational or administrative advantages of using local ingredients in “increased number of local cuisine in the menu” and “securing food safety” but showed low awareness on problems related to using local produce to cook for entire student body such as “longer cooking time” and “not enough providers.” Correlation between questionnaire items revealed that there was a strong positive relation between items emphasizing advantages of using local produce. The results showed that using local produce for providing school lunches could induce changes in behaviors, such as frequenting local produce markets, and it is considered to become a good evaluation index for developing shokuiku

Functional Expression of Heme Oxygenase-1 in Human Differentiated Epidermis and Its Regulation by Cytokines

Although heme oxygenase-1 (HO-1) is induced in keratinocytes after UV radiation, HO-1 expression during normal epidermal differentiation has not yet been reported. We showed by real-time PCR, western blotting, and ELISA that HO-1 mRNA and protein expression by cultured normal human keratinocytes was upregulated during epidermal differentiation induced by a high-calcium medium. Immunohistochemical staining and in situ hybridization showed the graduated expression of HO-1 in the upper epidermis, which was accompanied by suprabasal HO-1 mRNA expression, and the accumulation of bilirubin (BR) in the stratum corneum. We examined the activation of nuclear factor E2-related factor 2 (Nrf2), which is a pivotal transcription factor for HO-1 expression, by western blotting and by examining the mRNA expression of Nrf2 target genes, and excluded its role in HO-1 expression in epidermal differentiation. Next, we examined the regulation of HO-1 expression by inflammatory cytokines. IL-4 and IL-22 significantly reduced HO-1 mRNA and protein expression, whereas IL-1β, IL-17A, and tumor necrosis factor-α (TNF-α) increased it. Finally, immunohistochemical studies on psoriatic lesional skin showed that HO-1 expression was downregulated in the parakeratotic epidermis, whereas it was retained in the orthokeratotic epidermis. These studies demonstrate that HO-1 is functionally expressed by keratinocytes in parallel with epidermal differentiation and that its expression is independently affected by several cytokines

Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light

Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation

Pseudolymphomatous Folliculitis on the Nose

Pseudolymphomatous folliculitis (PLF), which sometimes mimicks cutaneous lymphoma, is a rare manifestation of cutaneous pseudolymphoma and cutaneous lymphoid hyperplasia. We describe a 57-year-old Japanese woman with PLF on the nose that resembled cutaneous lymphoma clinically. The biopsy specimen revealed dense lymphocytes, especially CD1a+ cells, infiltrated around the hair follicles. Without any additional treatment, her nodule rapidly decreased before we performed a second biopsy for analysis of the clonal gene rearrangement. Though PLF typically behaves as benign lymphohyperplasia, differentiation from cutaneous lymphoma is necessary