11 research outputs found
ΠΠ΅ΡΠ΅ΠΊΡΡ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΠΌΠΈ ΡΡΠ½ΠΊΡΠΈΠΈ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° (ΠΏΠΈΠ»ΠΎΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅)
The aim of the paper: to identify promising diagnostic and prognostic biomarkers of pathological processes development based on the red blood cell membrane morphology and nanostructure in patients with brain disorders in the Intensive Care Unit.Materials and methods. The study included 24 patients from the anesthesiology and resuscitation ward of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology. Blood was acquired from the patients for standard tests, and all further tests were performed in vitro. The images of red blood cells were obtained using the atomic force microscope Β«NTEGRA Π rimaΒ» (NT-MDT, Russia) in semi-contact mode.Results. Patients from the anesthesiology and intensive care ward with traumatic brain injury, ischemic and hemorrhagic stroke, cerebral edema, and post-hypoxic encephalopathy had different blood cell shapes and localized defects of different topology on the surface of erythrocyte membranes including defects of pallor, torus, and nanostructure.Conclusion. In this pilot study we have shown that several defects represent the trigger mechanisms for the development of a total membrane damage. Local topographic defects of nanostructures and abnormalities of erythrocyte morphology are irreversible. The number and quality of these abnormalities may eventually be used as a diagnostic and prognostic biomarker of pathological processes.Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ. ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ Π½Π°Π½ΠΎΡΡΡΡΠΊΡΡΡΡ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΠΌΠΈ ΡΡΠ½ΠΊΡΠΈΠΈ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, Π½Π°Ρ
ΠΎΠ΄ΡΡΠΈΡ
ΡΡ Π² ΠΎΡΠ΄Π΅Π»Π΅Π½ΠΈΠΈ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ ΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΈ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΊΠ»ΡΡΠΈΠ»ΠΈ 24 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° ΠΎΡΠ΄Π΅Π»Π΅Π½ΠΈΡ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ ΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΈ Π€ΠΠΠ¦ Π Π . ΠΡΠΎΠ²Ρ Π·Π°Π±ΠΈΡΠ°Π»ΠΈ Ρ Π΄Π°Π½Π½ΡΡ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Π΄Π»Ρ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΡ
Π°Π½Π°Π»ΠΈΠ·ΠΎΠ², ΠΈ Π²ΡΠ΅ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ in vitro. ΠΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΡ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π°ΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΠΈΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠ° Β«NTEGRA Π rimaΒ» (NT-MDT, Π ΠΎΡΡΠΈΡ) Π² ΠΏΠΎΠ»ΡΠΊΠΎΠ½ΡΠ°ΠΊΡΠ½ΠΎΠΌ ΡΠ΅ΠΆΠΈΠΌΠ΅.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΎΡΠ΄Π΅Π»Π΅Π½ΠΈΡ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ ΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΈ Ρ ΡΠ΅ΡΠ΅ΠΏΠ½ΠΎ-ΠΌΠΎΠ·Π³ΠΎΠ²ΠΎΠΉ ΡΡΠ°Π²ΠΌΠΎΠΉ, ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈ Π³Π΅ΠΌΠΎΡΡΠ°Π³ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈΠ½ΡΡΠ»ΡΡΠΎΠΌ, ΠΎΡΠ΅ΠΊΠΎΠΌ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, ΠΏΠΎΡΡΠ³ΠΈΠΏΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΠ΅ΠΉ ΠΈΠΌΠ΅Π»ΠΈ ΠΌΠ΅ΡΡΠΎ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠ΅ ΡΠΎΡΠΌΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΊΡΠΎΠ²ΠΈ, Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Π²ΠΎΠ·Π½ΠΈΠΊΠ°Π»ΠΈ Π»ΠΎΠΊΠ°Π»ΡΠ½ΡΠ΅ Π΄Π΅ΡΠ΅ΠΊΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΡΠΎΠΏΠΎΠ»ΠΎΠ³ΠΈΠΈ: Π΄Π΅ΡΠ΅ΠΊΡΡ ΠΏΡΠ»Π»ΠΎΡΠ°, ΡΠΎΡΠ°, Π½Π°Π½ΠΎΡΡΡΡΠΊΡΡΡΡ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π Π΄Π°Π½Π½ΠΎΠΌ ΠΏΠΈΠ»ΠΎΡΠ½ΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΌΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ ΡΡΠ΄ Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² ΡΠ²Π»ΡΡΡΡΡ ΡΡΠ°ΡΡΠΎΠ²ΡΠΌ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΎΡΠ°Π»ΡΠ½ΡΡ
ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½. ΠΠΎΠΊΠ°Π»ΡΠ½ΡΠ΅ ΡΠΎΠΏΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π΄Π΅ΡΠ΅ΠΊΡΡ Π½Π°Π½ΠΎΡΡΡΡΠΊΡΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Π½Π΅ΠΎΠ±ΡΠ°ΡΠΈΠΌΡ. ΠΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΈ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎ ΡΡΠΈΡ
Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ Π² ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π΅ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΊΠ°ΠΊ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅Ρ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ²
Defects of Red Blood Cell Membranes in Patients with Brain Dysfunction (Pilot Study)
The aim of the paper: to identify promising diagnostic and prognostic biomarkers of pathological processes development based on the red blood cell membrane morphology and nanostructure in patients with brain disorders in the Intensive Care Unit.Materials and methods. The study included 24 patients from the anesthesiology and resuscitation ward of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology. Blood was acquired from the patients for standard tests, and all further tests were performed in vitro. The images of red blood cells were obtained using the atomic force microscope Β«NTEGRA Π rimaΒ» (NT-MDT, Russia) in semi-contact mode.Results. Patients from the anesthesiology and intensive care ward with traumatic brain injury, ischemic and hemorrhagic stroke, cerebral edema, and post-hypoxic encephalopathy had different blood cell shapes and localized defects of different topology on the surface of erythrocyte membranes including defects of pallor, torus, and nanostructure.Conclusion. In this pilot study we have shown that several defects represent the trigger mechanisms for the development of a total membrane damage. Local topographic defects of nanostructures and abnormalities of erythrocyte morphology are irreversible. The number and quality of these abnormalities may eventually be used as a diagnostic and prognostic biomarker of pathological processes
Functionalisation of graphene as a tool for developing nanomaterials with predefined properties
Graphene based nanomaterials (GBN) have been recently applied in a broad range of science and technology fields such as nanobiomedicine, electronics, energy storage and power generation exploiting their unique electronic structure, physical properties, and opportunities for modifying their surface using covalent and non-covalent interactions. In the present review we systematised the origins of GBN functionalisation using organic and inorganic molecules, polymers, biomolecules, and anticancer drugs. We show that varying the procedure of GBN functionalisation allows to obtain nanomaterials with desired properties that can be applied to the development of materials with enhanced physicochemical properties, nanoplatforms for drug delivery, nanobiosensors for detection of various biomolecules, as well as nanomaterials for bioimaging and diagnostics. The review can be useful for experts in the fields of material science and nanobiomedicine
Endometallofullerenes and their derivatives:Synthesis, physicochemical properties, and perspective application in biomedicine
We review all aspects of the synthesis, properties, and applications of endometallofullerenes (EMF) over the last 20 years. EMF represent a new class of nanosized objects with specific physicochemical properties defined by the encapsulation of individual atoms and small molecules with significant potential of practical applications in biology and medicine. One such application is an EMF based anticancer drug capable of activating antitumour immune response, inhibition of the tumour development, and inhibition of angiogenesis and metastasis. Another, separate application direction is the use of EMF as contrast agents
Covalent conjugates based on nanodiamonds with doxorubicin and a cytostatic drug from the group of 1,3,5-triazines::Synthesis, biocompatibility and biological activity
We report the synthesis of covalent conjugates of nanodiamonds with doxorubicin and a cytostatic drug from the class of 1,3,5-triazines. The obtained conjugates were identified using a number of physicochemical methods (IR-spectroscopy, NMR-spectroscopy, XRD, XPS, TEM). As a result of our study, it was found that ND-Π‘ONH-Dox and ND-COO-Diox showed good hemocompatibility, since they did not affect plasma coagulation hemostasis, platelet functional activity, and erythrocyte membrane. The ND-COO-Diox conjugates are also capable of binding to human serum albumin due to the presence of ND in their composition. In the study of the cytotoxic properties of ND-Π‘ONH-Dox and ND-COO-Diox in the T98G glioblastoma cell line, indicating that ND-Π‘ONH-Dox and ND-COO-Diox demonstrate greater cytotoxicity at lower concentrations of Dox and Diox in the composition of the conjugates compared to individual drugs; the cytotoxic effect of ND-COO-Diox was statistically significantly higher than that of ND-Π‘ONH-Dox at all concentrations studied. Greater cytotoxicity at lower concentrations of Dox and Diox in the composition of conjugates compared to individual cytostatics makes it promising to further study the specific antitumor activity and acute toxicity of these conjugates in models of glioblastoma in vivo. Our results demonstrated that ND-Π‘ONH-Dox and ND-COO-Diox enter HeLa cells predominantly via a nonspecific actin-dependent mechanism, while for ND-Π‘ONH-Dox a clathrin-dependent endocytosis pathway. All data obtained provide that the synthesized nanomaterials show a potential application as the agents for intertumoral administration. [Abstract copyright: Copyright Β© 2023. Published by Elsevier B.V.
Protective action of water-soluble fullerene adducts on the example of an adduct with L-arginine
We present radioprotective, antiglycating, and photoprotective properties of a water-soluble C60 fullerene derivative with L-arginine (C60-Arg) and composite films based on collagen containing C60-Arg. The synthesis of these materials is described. The identification of the synthesised materials was carried out using modern physicochemical methods of analysis. The physicochemical properties of aqueous solutions of C60-Arg, such as, particle size distribution, zeta potentials, distribution coefficient in the octan-1-olβwater system were measured. The computer simulation of the process of C60-Arg association in aqueous and isotonic solutions was carried out using Molecular Dynamics. Composite films based on collagen containing C60-Arg demonstrate significant improvement in mechanical properties, cell adhesion, and cell proliferation when the nano-modifier is added. This shows high potential for the use of the C60-Arg adduct in biomedicine