Paramagnonlike excitations and spin diffusion in magnetic resonance studies of copper oxide superconductors

The relaxation function theory for a doped two-dimensional Heisenberg antiferromagnetic system in the paramagnetic state for all wave vectors through the Brillouin zone is presented in view of low frequency response of high-$T_c$ copper oxide superconductors. We deduced the regions of long lifetime [$T \lesssim 400(1-4x)$ K] and "overdamped" [$T \gtrsim 700(1-4x)$ K] paramagnonlike excitations in the temperature ($T$)-doping index ($x$) phase diagram from plane oxygen nuclear spin-lattice relaxation rate $^{17}(1/T_1)$ data in up to optimally doped La$_{2-x}$Sr$_{x}$CuO$_{4}$ thus providing the regimes for the spin wave concept and the ''overdamped'' mode.Comment: Physical Review B, accepted, in pres

The charge-assisted hydrogen-bonded organic framework (CAHOF) self-assembled from the conjugated acid of tetrakis(4-aminophenyl)methane and 2,6-naphthalenedisulfonate as a new class of recyclable Brønsted acid catalysts

The acid–base neutralization reaction of commercially available disodium 2,6-naphthalenedisulfonate (NDS, 2 equivalents) and the tetrahydrochloride salt of tetrakis(4-aminophenyl)methane (TAPM, 1 equivalent) in water gave a novel three-dimensional charge-assisted hydrogen-bonded framework (CAHOF, F-1). The framework F-1 was characterized by X-ray diffraction, TGA, elemental analysis, and 1H NMR spectroscopy. The framework was supported by hydrogen bonds between the sulfonate anions and the ammonium cations of NDS and protonated TAPM moieties, respectively. The CAHOF material functioned as a new type of catalytically active Brønsted acid in a series of reactions, including the ring opening of epoxides by water and alcohols. A Diels–Alder reaction between cyclopentadiene and methyl vinyl ketone was also catalyzed by F-1 in heptane. Depending on the polarity of the solvent mixture, the CAHOF F-1 could function as a purely heterogeneous catalyst or partly dissociate, providing some dissolved F-1 as the real catalyst. In all cases, the catalyst could easily be recovered and recycled

Physiological Aspects of Rat Activity, Their Anxiety and Memory After Administration of Full Gabaa-receptor Complex Agonist Propoxazepam

The action of 7-bromo-5 (o-chlorophenyl) -3-propoxy-1,2-dihydro-3H-1,4-benzodiazepin-2-one (propoxazepam) with prolonged administration in various doses on behavioural reactions, anxiety and the memory of rats, as well as their muscle tone was estimated, which is important paying attention to its main (anticonvulsant and analgesic) pharmacological effects.The aim of the study was a comparative assessment of the severity and duration of propoxazepam effect after its administration to rats at doses of 2, 5 and 10 mg/kg (10 days) on higher functions of the central nervous system according to indicators such as motor and exploratory activity, as well as reference and working memory .Materials and methods. The study was conducted on 40 Wistar rats weighing 220-290 g. The psychophysiological state of the animals was evaluated using the "open field" test, the formation of spatial working and long-term memory in a radial eight-arm maze, muscle relaxation, imbalance and movements coordination - using the "rotating rod" method. Statistical processing was performed in Microsoft Excel 2016 with AtteStat 12.The results of the study. When comparing the test results of the "open field", "radial labyrinth" and "rotarod" in groups of animals that were administered different doses of the compound, it was found that, in general, these parameters at doses of 2 mg/kg and 5 mg/kg statistically significantly different (increase) from control values, but are similar. At a dose of 10 mg/kg, most of the parameters (except for the rotarod test) for these animals were characterized by a downward trend.Conclusions. The administration of the drug in doses of 2, 5 and 10 mg/kg leads to a decrease in anxious behaviour of animals, which is also accompanied by a pronounced dose-dependent negative effect on the endurance, coordination and memory of animals. The administration of the drug at a dose of 10 mg/kg impaired the learning ability of animals and reduced memory function. This should be considered in clinical studies of the compoun

AFM and FTIR Investigation of the Effect of Water Flow on Horseradish Peroxidase

Atomic force microscopy (AFM)-based fishing is a promising method for the detection of low-abundant proteins. This method is based on the capturing of the target proteins from the analyzed solution onto a solid substrate, with subsequent counting of the captured protein molecules on the substrate surface by AFM. Protein adsorption onto the substrate surface represents one of the key factors determining the capturing efficiency. Accordingly, studying the factors influencing the protein adsorbability onto the substrate surface represents an actual direction in biomedical research. Herein, the influence of water motion in a flow-based system on the protein adsorbability and on its enzymatic activity has been studied with an example of horseradish peroxidase (HRP) enzyme by AFM, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and conventional spectrophotometry. In the experiments, HRP solution was incubated in a setup modeling the flow section of a biosensor communication. The measuring cell with the protein solution was placed near a coiled silicone pipe, through which water was pumped. The adsorbability of the protein onto the surface of the mica substrate has been studied by AFM. It has been demonstrated that incubation of the HRP solution near the coiled silicone pipe with flowing water leads to an increase in its adsorbability onto mica. This is accompanied by a change in the enzyme’s secondary structure, as has been revealed by ATR-FTIR. At the same time, its enzymatic activity remains unchanged. The results reported herein can be useful in the development of models describing the influence of liquid flow on the properties of enzymes and other proteins. The latter is particularly important for the development of biosensors for biomedical applications—particularly for serological analysis, which is intended for the early diagnosis of various types of cancer and infectious diseases. Our results should also be taken into account in studies of the effects of protein aggregation on hemodynamics, which plays a key role in human body functioning

AFM and FTIR Investigation of the Effect of Water Flow on Horseradish Peroxidase

Atomic force microscopy (AFM)-based fishing is a promising method for the detection of low-abundant proteins. This method is based on the capturing of the target proteins from the analyzed solution onto a solid substrate, with subsequent counting of the captured protein molecules on the substrate surface by AFM. Protein adsorption onto the substrate surface represents one of the key factors determining the capturing efficiency. Accordingly, studying the factors influencing the protein adsorbability onto the substrate surface represents an actual direction in biomedical research. Herein, the influence of water motion in a flow-based system on the protein adsorbability and on its enzymatic activity has been studied with an example of horseradish peroxidase (HRP) enzyme by AFM, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and conventional spectrophotometry. In the experiments, HRP solution was incubated in a setup modeling the flow section of a biosensor communication. The measuring cell with the protein solution was placed near a coiled silicone pipe, through which water was pumped. The adsorbability of the protein onto the surface of the mica substrate has been studied by AFM. It has been demonstrated that incubation of the HRP solution near the coiled silicone pipe with flowing water leads to an increase in its adsorbability onto mica. This is accompanied by a change in the enzyme’s secondary structure, as has been revealed by ATR-FTIR. At the same time, its enzymatic activity remains unchanged. The results reported herein can be useful in the development of models describing the influence of liquid flow on the properties of enzymes and other proteins. The latter is particularly important for the development of biosensors for biomedical applications—particularly for serological analysis, which is intended for the early diagnosis of various types of cancer and infectious diseases. Our results should also be taken into account in studies of the effects of protein aggregation on hemodynamics, which plays a key role in human body functioning

Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma

Application of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of «silicon-on-insulator» (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litography with gas-phase etching. The so-fabricated SOI-NW chips are intended for highly sensitive detection of brain cancer biomarkers in humans. In our present study, two series of experiments have been conducted. In the first experimental series, detection of a synthetic DNA oligonucleotide (oDNA) analogue of brain cancer-associated microRNA miRNA-363 in purified buffer solution has been performed in order to demonstrate the high detection sensitivity. The second experimental series has been performed in order to reveal miRNA-363 itself in real human plasma samples. To provide detection biospecificity, the SOI-NW chip surface was modified by covalent immobilization of probe oligonucleotides (oDNA probes) complementary to the target biomolecules. Using the SOI-NW sensor chips proposed herein, the concentration detection limit of the target biomolecules at the level of 3.3 × 10−17 M has been demonstrated. Thus, the approach employing the SOI-NW chips proposed herein represents an attractive tool in biomedical practice, aimed at the early revelation of oncological diseases in humans