26 research outputs found
Genetic and molecular characterization of three novel S-haplotypes in sour cherry (Prunus cerasus L.)
Tetraploid sour cherry (Prunus cerasus L.) exhibits gametophytic self-incompatibility (GSI) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, S-RNase and SFB (S haplotype-specific F-box protein gene), respectively. As sour cherry selections can be either self-compatible (SC) or self-incompatible (SI), polyploidy per se does not result in SC. Instead the genotype-dependent loss of SI in sour cherry is due to the accumulation of non-functional S-haplotypes. The presence of two or more non-functional S-haplotypes within sour cherry 2x pollen renders that pollen SC. Two new S-haplotypes from sour cherry, S33 and S34, that are presumed to be contributed by the P. fruticosa species parent, the complete S-RNase and SFB sequences of a third S-haplotype, S35, plus the presence of two previously identified sweet cherry S-haplotypes, S14 and S16 are described here. Genetic segregation data demonstrated that the S16-, S33-, S34-, and S35-haplotypes present in sour cherry are fully functional. This result is consistent with our previous finding that āhetero-allelicā pollen is incompatible in sour cherry. Phylogenetic analyses of the SFB and S-RNase sequences from available Prunus species reveal that the relationships among S-haplotypes show no correspondence to known organismal relationships at any taxonomic level within Prunus, indicating that polymorphisms at the S-locus have been maintained throughout the evolution of the genus. Furthermore, the phylogenetic relationships among SFB sequences are generally incongruent with those among S-RNase sequences for the same S-haplotypes. Hypotheses compatible with these results are discussed
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Genetic and molecular characterization of three novel S-haplotypes in sour cherry (Prunus cerasus L.).
Tetraploid sour cherry (Prunus cerasus L.) exhibits gametophytic self-incompatibility (GSI) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, S-RNase and SFB (S haplotype-specific F-box protein gene), respectively. As sour cherry selections can be either self-compatible (SC) or self-incompatible (SI), polyploidy per se does not result in SC. Instead the genotype-dependent loss of SI in sour cherry is due to the accumulation of non-functional S-haplotypes. The presence of two or more non-functional S-haplotypes within sour cherry 2x pollen renders that pollen SC. Two new S-haplotypes from sour cherry, S(33) and S(34), that are presumed to be contributed by the P. fruticosa species parent, the complete S-RNase and SFB sequences of a third S-haplotype, S(35), plus the presence of two previously identified sweet cherry S-haplotypes, S(14) and S(16) are described here. Genetic segregation data demonstrated that the S(16)-, S(33)-, S(34)-, and S(35)-haplotypes present in sour cherry are fully functional. This result is consistent with our previous finding that 'hetero-allelic' pollen is incompatible in sour cherry. Phylogenetic analyses of the SFB and S-RNase sequences from available Prunus species reveal that the relationships among S-haplotypes show no correspondence to known organismal relationships at any taxonomic level within Prunus, indicating that polymorphisms at the S-locus have been maintained throughout the evolution of the genus. Furthermore, the phylogenetic relationships among SFB sequences are generally incongruent with those among S-RNase sequences for the same S-haplotypes. Hypotheses compatible with these results are discussed
Phylogenetic trees based on phylogenetic analysis of nucleotide sequences of (left) and (right) alleles for species of
Numbers above, below, or adjacent to branches are bootstrap support values greater than 50%; asterisks indicate branches that collapsed in the strict consensus tree from each analysis. The positions of PcS33, PcS34, and PcS35 are indicated, respectively, by open boxes, lightly shaded boxes, and darkly shaded boxes. Left: Single most parsimonious tree (l=2366, ci excluding autapomorphies=0.3271, ri=0.5263) based on alleles. The nucleotide sequences for three novel . () alleles presented in this study were aligned with 12 alleles from . (, AB028153; , AB010304; , AB010306; , AB028154; , AJ298314; , AB010305; , EU035974; , AY259115; , DQ385842; , AY259114; , AY259112; , AY259113); one from . (, EU035975); three from . (, AM746946; , AM746947; , AM746948); nine from . (, AB026836; , AB011469; , AB011470; , AB011471; , AB252409; , DQ099895; , DQ093825; , AM231660; , AM746949); two from . (, AB101438; , AB101439); four from . (, AY587561; , AY587562; , AY587564; , EU516388); three from . (, AM746943; , AM746944; , AM746945); ten from . (, AB252411; , AB252413; , AB084102; , AB084103; , AB280693; , DQ512911; , AM746950; , DQ512914; , AY781290; , DQ512913); seven from . (, DQ677587; , DQ677588; , DQ677589; , DQ677590; , DQ677584; , DQ677585; , DQ677586); and nine from . (, DQ983373; , DQ983374; , DQ983375; , DQ983363; , DQ983364; , DQ983365; , DQ983366; , DQ983367; , DQ983370). Data set contained 63 taxa and 744 characters, of which 220 were constant, 129 were variable but uninformative, and 395 were parsimony-informative. Right: One of 65 most parsimonious trees (lā=ā2968, ci excluding autapomorphiesā=ā0.3913, riā=ā0.4860) from phylogenetic analysis of nucleotide sequences of alleles. The nucleotide sequences for three novel . () alleles presented in this study were aligned with coding sequences for 12 alleles from . (, AY805048; , AB111519; , AB096857; , AB111521; , AB111520; , AB096858; , EU035976; , DQ422809; , AY805053; , AY805054; , DQ385844; , AY805056); two from . (, DQ827715; , EU035977); three from . (, AM746955; , AM746956; , AM746957); seven from . (, AB092966; , AB092967; , AB079776; , AB081648; , AB252408; , EF061758; , AM746959); three from . (, AB101440; , AB101441; , AB092645); four from . (, AY587563; , AY587562; , AY587565; , EU516388); three from . (, AM746952; , AM746953; , AM746954); nine from . (, AB252410; , AB252412; , DQ849084; , AM746962; , AB280794; , DQ849089; , AM746963; , DQ849118; , DQ849085); seven from . (, DQ677587; , DQ677588; , DQ677589; , DQ677598; , DQ677616; , DQ677615; , DQ677595), and one from . (, DQ983369). Data set contained 54 taxa and 1161 characters, of which 337 were constant, 230 were variable but uninformative, and 594 were parsimony-informative.<p><b>Copyright information:</b></p><p>Taken from "Genetic and molecular characterization of three novel -haplotypes in sour cherry ( L.)"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3169-3185.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2504349.</p><p></p
Amino acid sequence alignment of three novel SFBs obtained from sour cherry and that of other functional SFBs from sour cherry
The alignment was generated by DNASIS version 3.5 (Hitachi Software Engineering Co. Ltd., Tokyo, Japan). Gaps are marked by dashes. Conserved amino acids are shown on a darkened background. The locations of the F-box motif, V1, V2, HVa, and HVb (), and Vn () are indicated by solid boxes.<p><b>Copyright information:</b></p><p>Taken from "Genetic and molecular characterization of three novel -haplotypes in sour cherry ( L.)"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3169-3185.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2504349.</p><p></p
PCR amplification with -, -, and -allele specific primer pair for and in 17 sour cherry selections
PCR products were separated on 2% agarose gel and detected with ethidium bromide staining. M, 123 bp DNA ladder (Invitrogen, Carlsbad, CA, USA). Lane abbreviations are: C59, āCigĆ”ny 59ā; Cri, āCrisanaā; ET, āEnglaise Timpuriiā; EB, āErdi Botermoā; EJ, āErdi Jubileumā; EN, āErdi Nagygyumolcsuā; Met, āMeteorā; Mon, āMontmorencyā; P38, āPandy 38ā; P114, āPandy 114ā; RS, āRheinische Schattenmorelleā; Sur, āSurefireā; Tam, āTamarisā; Tar, āTarinaā; Tsc, āTschernokorkaā; UF, āĆjfehĆ©rtĆ³i fűrtÅsā; III 18 (12), āMSU III 18 (12)ā.<p><b>Copyright information:</b></p><p>Taken from "Genetic and molecular characterization of three novel -haplotypes in sour cherry ( L.)"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3169-3185.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2504349.</p><p></p
Validating markers linked to soluble solid contents in octoploid strawberry
Validating markers linked to soluble solid contents in octoploid strawberry . 2014 ASHS Annual Conferenc