Kafa travmasında deksmedetomidin ve propofolün antinöroapopitotik etkilerinin karşılaştırılması: hayvan Çalışması

Objective: Traumatic brain injury (TBI) is a common consequence of accidents, and apoptosis is now recognized as one of its important pathophysiological factors. The primary hypothesis of this study was to show the early antineuroapoptotic effects of propofol and dexmedetomidine by showing the small number of apoptotic cells after mild TBI. Material and Methods: Forty five rats, anesthetized with intraperitoneal 50mg/kg ketamine hydrocloride and 5mg/kg xylazine, were randomly assigned into 5 groups. Groups 1 (trauma) and 2 (no trauma) were applied propofol while Groups 3 (trauma) and 4 (no trauma) were applied dexmedetomidine. No additional anesthetics were applied to Group 5 (trauma). The mean arterial pressure (MAP), rectal temperature and blood glucose levels were monitored for 2 hours. Then, the brains of the rats were removed after sacrification and craniectomy, and the apoptotic cell analysis was done in midsagital, parasagittal and hippocampal regions. Results: The median values for mean body weight, MAP, and temperature were similar(p>0.05), but glucose levels were significantly higher in Group 5 in the first 45 min (p0.05). Conclusion: The number of apoptotic cells in rat brains with mild TBI, in which propofol and dexmedetomidine applied, was smaller. However, these two agents had no superiority to each other in terms of antineuroapoptotic effect. These agents were thought to be protective against the early phase brain damage. © 2014 by Türkiye Klinikleri

Comparative Antineuroapoptotic Effects of Dexmedetomidine and Propofol in Cranial Injury: An Animal Study

Objective: Traumatic brain injury (TBI) is a common consequence of accidents, and apoptosis is now recognized as one of its important pathophysiological factors. The primary hypothesis of this study was to show the early antineuroapoptotic effects of propofol and dexmedetomidine by showing the small number of apoptotic cells after mild TBI. Material and Methods: Forty five rats, anesthetized with intraperitoneal 50mg/kg ketamine hydrocloride and 5mg/kg xylazine, were randomly assigned into 5 groups. Groups 1 (trauma) and 2 (no trauma) were applied propofol while Groups 3 (trauma) and 4 (no trauma) were applied dexmedetomidine. No additional anesthetics were applied to Group 5 (trauma). The mean arterial pressure (MAP), rectal temperature and blood glucose levels were monitored for 2 hours. Then, the brains of the rats were removed after sacrification and craniectomy, and the apoptotic cell analysis was done in midsagital, parasagittal and hippocampal regions. Results: The median values for mean body weight, MAP, and temperature were similar(p>0.05), but glucose levels were significantly higher in Group 5 in the first 45 min (p0.05). Conclusion: The number of apoptotic cells in rat brains with mild TBI, in which propofol and dexmedetomidine applied, was smaller. However, these two agents had no superiority to each other in terms of antineuroapoptotic effect. These agents were thought to be protective against the early phase brain damage. © 2014 by Türkiye Klinikleri

Therapeutic Efficiency of Tibolone in a Rat Endometriosis Model

Objective: This study aimed to evaluate the therapeutic efficiency of tibolone in an experimental rat model of intra-abdominal endometriosis. Material and Methods: In this experimental study, intra-abdominal endometriosis was induced in 30 female rats surgically. Four weeks after this procedure, laparotomy was performed again. The dimensions of the endometriosis foci were recorded and right after, the operation was completed. Rats were randomly divided into three groups and subsequently, the treatment was started. In the first group (n=8), a single dose of 1 cc 0.9% NaCl was injected subcutaneously. In the second group (n=8), intramuscular injection of 1 mg leuprolid acetate was administered. In the third group (n=8), 1 mg/kg/day of tibolone was given by gavage. At the end of 4- weeks of drug administration, laparotomy was performed to all rats. The dimensions of the endometriosis foci were recorded. Afterwards, all the rats were sacrificed. The differences in the areas of endometriotic implants and adhesion scores were compared between the groups. Results: The dimensions of the endometriosis foci in the groups treated with leuprolid (p<0.05) and tibolone (p<0.05) were significantly diminished compared to that of the control group. No statistically significant differences were found between the treatment groups. Conclusion: In a rat endometriosis model, tibolone has a similar efficiency as that of leuprolid acetate, an agent used for conventional medical treatment of endometriosis. With its androgenic and progestagenic characteristics, tibolone deserves attention as an alternative agent in the medical treatment of endometriosis in human

Gelatin Based Scaffolds and Effect of EGF Dose on Wound Healing

In this study, gelatin based biodegradable scaffolds (GS) containing various amounts of epidermal growth factor (EGF) were prepared and applied to full skin defects created on rats in order to investigate the EGF dose effect on healing process. Scaffolds were prepared by freeze drying technique and stabilized with either glutaraldehyde (GA) or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC). In vitro cytotoxicity of the prepared scaffolds was assessed by using human skin fibroblast secondary culture (HS2). Scaffolds crosslinked with GA which showed no cytotoxicity in vitro were selected for in vivo applications, loaded with different amounts of EGF (1 mu g, 5 mu g or 10 mu g per cm(3) of scaffold) and were applied onto full skin defects created on rats. Decrease in the wound size and level of reepithelization were examined and the results showed that, all scaffolds, with or without EGF, demonstrated a potential to enhance wound healing. Healing process became faster and better parallel to the dose of EGF present in the scaffolds. Although wounds demonstrated better and faster healing parallel to the EGF content, the dose should be optimized through examining the effects in molecular level before any clinical application