In Vitro And In Vivo Studies Of Novel Poly(d,l-lactic Acid), Superhydrophilic Carbon Nanotubes, And Nanohydroxyapatite Scaffolds For Bone Regeneration

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Poly(D,L-lactide acid, PDLLA) has been researched for scaffolds in bone regeneration. However, its hydrophobocity and smooth surface impedes its interaction with biological fluid and cell adhesion. To alter the surface characteristics, different surface Modification techniques have been developed to facilitate biological application. The present study compared two different routes to produce PDLLA/superhydrophilic vertically aligned carbon nanotubes:nanohydroxyapatite (PDLLA/VACNT-O:nHAp) scaffolds. For this, we used electrodeposition and immersion in simulated body fluid (SBF). Characterization by goniometry, scanning electron microscopy, X-ray diffraction, and infrared spectroscopy confirmed the polymer modifications, the in vitro bioactivity, and biomineralization. Differential sunning calorimetry and thermal gravimetric analyses showed that the inclusion of VACNT-O:nHA probably acts as a nucleating agent increasing the crystallization rate in the neat PDLLA without structural alteration. Our results showed the formation of a dense nHAp layer on all scaffolds after 14 days of immersion in SBF solution; the most intense carbonated nHAp peaks observed in the PDLLA/VACNT-O:nHAp samples suggest higher calcium precipitation compared to the PDLLA control. Both cell viability and alkaline phosphatase assays showed favorable results, because no cytotoxic effects were present and all produced scaffolds were able to induce detectable mineralization. Bone defects were used to evaluate the bone regeneration; the confocal Raman and histological results confirmed high potential for bone applications: In vivo study showed that the PDLLA/VACNT-O:nHAp scaffolds mimicked the immature bone and induced bone remodeling: These findings indicate surface improvement and the applicability of this new nanobiomaterial for hone regenerative medicine.71893859398Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Innovation Agency (FINEP) [01-13-0428-00, 1259/13]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP [2011/17877-7, 2011/20345-7, 2012/02159-4]CNPq [474090/2013-2, 307809/2013-7

Porous membranes of the polycaprolactone (PCL) containing calcium silicate fibers for guided bone regeneration

Porous membranes of polycaprolactone containing calcium silicate fibers for guided bone regeneration were prepared by a controlled humidity method. The results indicated the development of the novel membrane microstructure with porosity and surface roughness increased. The pore size varied between 4.76-14.28 mu m and the roughness increase 72.8% compared with control. These characteristics can provide a better interaction with the biological medium combined to the osteoconduction properties of the calcium silicate fibers and biocompatibility of the PCL. Therefore, the porous PCL/CaSiO3 membranes are promising biomaterial for guided bone regeneration. (C) 2017 Elsevier B.V. All rights reserved.Sao Paulo Research Foundation - FAPESP BrazilFed Univ Sao Paulo UNIFESP, 330 Talim St, BR-2231280 Sao Jose Dos Campos, SP, BrazilNatl Inst Space Res INPE, 1758 Astronautas Ave, BR-2227010 Sao Jose Dos Campos, SP, BrazilUniv Fed Sao Carlos, Rod Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, BrazilFed Univ Sao Paulo UNIFESP, 330 Talim St, BR-2231280 Sao Jose Dos Campos, SP, BrazilFAPESP: 2015/24659-7Web of Scienc