cpvoucherTREC2003

These data are for individual caterpillars 3rd instar and beyond collected from experimental plants and brought to the lab for rearing. Columns in the file are: Number (collection number, each caterpillar is a separate record, some multiple in one collection number - initially renumbered with decimals, that convention later abandoned); plant number (same as in other file); Collection date; Life History stage (instar); Caterpillar species (s.o. = sleepy orange, Abaeis nicippe; c.s. = cloudless sulfur, Phoebis sennae; o.b.s. = orange-barred sulfur, Phoebis philea); Pupated (yes/no); Pupation date; Adult M/F (No or sex); Parasitized; Virus; Moldy; Discontinued date; comments

Predators on <i>Senna chapmannii</i> plants—upper left, <i>Polistes major</i> wasp with <i>Phoebis philea</i> caterpillar; upper right, <i>Polistes</i> wasp damage to <i>Phoebis sennae</i> chrysalis; lower right, coccinelid <i>Brachiacantha decora</i> adult at extrafloral nectary; lower left, thomisid spider <i>Misumenoides formosipes</i> ready for prey.

<p>Predators on <i>Senna chapmannii</i> plants—upper left, <i>Polistes major</i> wasp with <i>Phoebis philea</i> caterpillar; upper right, <i>Polistes</i> wasp damage to <i>Phoebis sennae</i> chrysalis; lower right, coccinelid <i>Brachiacantha decora</i> adult at extrafloral nectary; lower left, thomisid spider <i>Misumenoides formosipes</i> ready for prey.</p

Arthropods on Senna mexicana var. chapmannii plants in plantation at TREC, Homestead, Florida 2003-2004

Plants were established, then manipulated throughout the growing season, and weekly observations were made of arthropods. Columns of data are: Plant number; date; block; treatment (1 = TF = tanglefoot; 2 = C = control; 3 = NP = nail polish; 4 = NPC = nail polish control); status (plant alive, sick, dead); sulfurcp (eggs and larvae of Pieridae); flysuck (Ceratopogonidae); tortricid (larvae tying stem tips); spider; bug predators; wasp predators; ladybug (Cocinellidae); fire ants (Solenopsis); Tiny brown ants (Brachymyrmex); Large Black Ants (Camponotus rasilis & C. sexguttatus); Odontomyrmex; crazy ants (Paratrechina); Camponotus abdominalis; weevils; Pseudomyrmex elongatus; Pseudomyrmex mexicanus (=P. gracilis); snakes (occasionally around or in plants). For all ant species, Ceratopogonidae, weevils, only presence/absence on plant was noted (1 = present)

Arthropods associated with experimental <i>Senna</i> plants.

<p>Table 1—Arthropods on <i>Senna mexicana</i> var. <i>chapmanii</i> plants in experimental plantation, Homestead, FL. Taxonomic information includes order, family, genus, species, and authority. Ant (Formicidae) species considered exotic are indicated with *. Brief ecological information is also provided for each documented association.</p><p>Arthropods associated with experimental <i>Senna</i> plants.</p

Ants on <i>Senna mexicana</i> var. <i>chapmanii</i> (experimental plantation) plants in Homestead, FL.

<p>Mean and standard error of numbers summed over the field season, over all plants, by treatment. Differences among treatments shown by Kruskal-Wallis for presence/absence data. Treatments abbreviated as C = control, TF = (tanglefoot) ant exclusion, NP = nail polish, NPC = nail polish control. Sample sizes of plants monitored were TF = 12, C = 15, NP = 12, and NPC = 11).</p

Some players in the tritrophic system—upper left, caterpillar of the orange-barred sulfur butterfly, <i>Phoebis philea</i>, on <i>Senna chapmanii</i>; upper right, pupa (chrysalis) of <i>P</i>. <i>sennae</i>; lower left, adult <i>P</i>. <i>sennae</i>; lower right: caterpillar studded with sucking flies (virus transmitters?).

<p>When viruses are involved, the pupae do not hatch, but instead turn various colors.</p

Rates of parasitization, virus death, and adulthood reached by caterpillars by treatment.

<p>Sample size is the number of caterpillars collected from plants (3^rd instar and beyond) and reared from plants in each treatment group. Parasitized counts include both definitely and “maybe parasitized” individuals.</p

Other potential predators on <i>Senna mexicana</i> var. <i>chapmanii</i> (experimental plantation) plants in Homestead, FL.

<p>Mean and standard error of numbers summed over the field season, over all plants, by treatment. Differences among treatments shown by ANOVA for count data. Treatments and sample sizes the same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138157#pone.0138157.g001" target="_blank">Fig 1</a>.</p

Development of an IAPV capture ELISA.

<p><b>A</b>. Pepscan of 4 monoclonal antibodies to IAPV VP2 on an overlapping peptide library. Filled bars IAPVMAb8; stipple bars IAPVMAb12; striped bars IAPVMAb17; open bars IAPVMAb 27. The relationship between peptide identity and VP2 structure is shown. <b>B</b>. Twin site capture ELISA using capture MAbs 8 (triangles) and 27 (diamonds) as capture layer and probing with HRP-labelled IAPV MAb12. The test sample was a lysate of Sf9 cell infected with the baculovirus expressing ORF-2-5TFS-3C.</p

The IAPV genome and origin of sequences used for IAPV fragment expression.

<p><b>A</b>. Genome structure of IAPV dicistrovirus with the two open reading frames and their constituent mature proteins shown. IRES – internal ribosome entry site, IGR – intergenic region. <b>B</b>. The fragments targeted for expression in <i>E.coli</i> and their encoded products. The precise endpoints used were: VP2, VTH→MQC; VP2-4, VTH→FGW; VP3, SKP→ELQ; VP1, INI→ISR. Expression screening for the proteins predicted from the fragments shown in B. <b>C</b>- left. Western blot with anti-His antibody. <b>C</b>-right. Purified VP2 used for the generation of a rabbit serum. Numbers to the left of the blot are protein molecular mass markers and are in kilodaltons.</p