We need to talk – a meander

Dr Ian Fraser reminds us, that despite modern cultural assumptions to the contrary, the Book of Genesis states that human beings are expressly forbidden ‘to work their own will on the world’. We are to fulfil God’s will, not ours, for the development of the world. On the basis of Genesis, he argues, human beings are not simply to subdue the Earth, but to take care of it and cultivate it. This cherishing of the Earth and its living beings is, therefore, our human responsibility. Referring to the development of the universe, Fraser explores the meaning of ‘creation’, and argues for the importance of dialogue between the disciplines. He returns to the text of the first chapters of Genesis which presages the history contained in the Old and New Testaments and concludes by offering a further five cosmological points for further consideration.Publisher PD

Auditors’ and PAC members’ views on performance auditing practices in Malaysia: a qualitative approach

This study explored the views of both auditors in the National Audit Department (NAD), and Public Accounts Committee (PAC) members toward the current performance-audit practices in the Malaysian public sector by interviewing selected members of each group. Results revealed that the views of these two groups diverged on several issues concerning auditor roles and responsibilities, auditor competence and independence, as well as the format, content and usefulness of the audit reports. The most significant divergence was in the respective views on the auditors' responsibilities for fraud detection

Design, validation and dissemination of an undergraduate assessment tool using SimMan® in simulated medical emergencies

Background: Increasingly, medical students are being taught acute medicine using whole-body simulator manikins. Aim: We aimed to design, validate and make widely available two simple assessment tools to be used with Laerdal SimMan (R) for final year students. Methods: We designed two scenarios with criterion-based checklists focused on assessment and management of two medical emergencies. Members of faculty critiqued the assessments for face validity and checklists revised. We assessed three groups of different experience levels: Foundation Year 2 doctors, third and final year medical students. Differences between groups were analysed, and internal consistency and interrater reliability calculated. A generalisability analysis was conducted using scenario and rater as facets in design. Results: A maximum of two items were removed from either checklist following the initial survey. Significantly different scores for three groups of experience for both scenarios were reported (p0.90). Internal consistency was poor (alpha<50.5). Generalizability study results suggest that four cases would provide reliable discrimination between final year students. Conclusions: These assessments proved easy to administer and we have gone some way to demonstrating construct validity and reliability. We have made the material available on a simulator website to enable others to reproduce these assessments

Mass segregation trends in SDSS galaxy groups

It has been shown that galaxy properties depend strongly on their host environment. In order to understand the relevant physical processes driving galaxy evolution it is important to study the observed properties of galaxies in different environments. Mass segregation in bound galaxy structures is an important indicator of evolutionary history and dynamical friction timescales. Using group catalogues derived from the Sloan Digital Sky Survey Data Release 7 (SDSS DR7) we investigate mass segregation trends in galaxy groups at low redshift. We investigate average galaxy stellar mass as a function of group-centric radius and find evidence for weak mass segregation in SDSS groups. The magnitude of the mass segregation depends on both galaxy stellar mass limits and group halo mass. We show that the inclusion of low mass galaxies tends to strengthen mass segregation trends, and that the strength of mass segregation tends to decrease with increasing group halo mass. We find the same trends if we use the fraction of massive galaxies as a function of group-centric radius as an alternative probe of mass segregation. The magnitude of mass segregation that we measure, particularly in high-mass haloes, indicates that dynamical friction is not acting efficiently.Comment: 6 pages, 2 figures, accepted for publication in MNRAS Letter

Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis.

BACKGROUND: RNA inhibition by siRNAs is a frequently used approach to identify genes required for specific biological processes. However RNAi screening using siRNAs is hampered by non-specific or off target effects of the siRNAs, making it difficult to separate genuine hits from false positives. It is thought that many of the off-target effects seen in RNAi experiments are due to siRNAs acting as microRNAs (miRNAs), causing a reduction in gene expression of unintended targets via matches to the 6 or 7 nt 'seed' sequence. We have conducted a careful examination of off-target effects during an siRNA screen for novel regulators of the TRAIL apoptosis induction pathway(s). RESULTS: We identified 3 hexamers and 3 heptamer seed sequences that appeared multiple times in the top twenty siRNAs in the TRAIL apoptosis screen. Using a novel statistical enrichment approach, we systematically identified a further 17 hexamer and 13 heptamer seed sequences enriched in high scoring siRNAs. The presence of one of these seeds sequences (which could explain 6 of 8 confirmed off-target effects) is sufficient to elicit a phenotype. Three of these seed sequences appear in the human miRNAs miR-26a, miR-145 and miR-384. Transfection of mimics of these miRNAs protects several cell types from TRAIL-induced cell death. CONCLUSIONS: We have demonstrated a role for miR-26a, miR-145 and miR-26a in TRAIL-induced apoptosis. Further these results show that RNAi screening enriches for siRNAs with relevant off-target effects. Some of these effects can be identified by the over-representation of certain seed sequences in high-scoring siRNAs and we demonstrate the usefulness of such systematic analysis of enriched seed sequences

Predicting success in graduate entry medical students undertaking a graduate entry medical program (GEM)

Background: Success in undergraduate medical courses in the UK can be predicted by school exit examination (A level) grades. There are no documented predictors of success in UK graduate entry medicine (GEM) courses. This study looks at the examination performance of GEM students to identify factors which may predict success; of particular interest was A level score. Methods: Data was collected for students graduating in 2004, 2005 and 2006, including demographic details (age and gender), details of previous academic achievement (A level total score and prior degree) and examination results at several points during the degree course. Results: Study group comprised 285 students. Statistical analyses identified no significant variables when looking at clinical examinations. Analysis of pass/fail data for written examinations showed no relationship with A level score. However, both percentage data for the final written examination and the analysis of the award of honours showed A level scores of AAB or higher were associated with better performance (p < 0.001). Discussion: A prime objective of introducing GEM programs was to diversify admissions to medical school. In trying to achieve this, medical schools have changed selection criteria. The findings in this study justify this by proving that A level score was not associated with success in either clinical examinations or passing written examinations. Despite this, very high achievements at A level do predict high achievement during medical school. Conclusions: This study shows that selecting graduate medical students with the basic requirement of an upper-second class honours degree is justifiable and does not disadvantage students who may not have achieved high scores in school leaver examinations