Complete chemical shift assignment of the isolated polysaccharides.

<p>N-acetyl signal: 2.04/25.0 ppm.</p><p>Carbon chemical shifts are in italics. Carbon chemical shifts with characteristic downfield displacement due to glycosylation are in bold.</p

Structure of capsule polysaccharide repeating unit (top) and galactan exopolysaccharide repeating unit (bottom).

<p>Structure of capsule polysaccharide repeating unit (top) and galactan exopolysaccharide repeating unit (bottom).</p

Colony morphology of <i>K. kingae</i> strains used in this study.

<p>Strain KK01 (A) and KK01<i>pamABCDE</i> (B) form mucoid colonies consistent with encapsulation. KK01<i>ctrA</i> (C) and KK01<i>ctrA pamABCDE</i> (D) form rough, non-mucoid colonies consistent with loss of encapsulation.</p

Strains used in this study ?.

<p>Strains used in this study ?.</p

2-D HSQC NMR spectrum of the polysaccharide with assignments of all the signals of the three major residues.

<p>For numbering, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075409#pone-0075409-t003" target="_blank">Table 3</a>.</p

Glycosyl composition of extracellular preparations of <i>K. kingae</i> strains used in this study.

<p>Mass in µg is listed first, and percentage is shown in parentheses. Mass and percentage are per 10 µg polysaccharide material analyzed.</p>*<p>3-Deoxy-D-Manno-oct-2-ulosonic acid.</p

2-D NOESY NMR spectrum of the polysaccharide.

<p>The sequence-determining correlations are labeled. For numbering, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075409#pone-0075409-t003" target="_blank">Table 3</a>.</p

Staining profile and purity of polysaccharide material used for analysis.

<p>Alcian blue staining is shown on the top, and silver staining is on the bottom. Lane 1, extract from KK01 using heat; lane 2, extract from KK01 using Tris acetate; lane 3, purified material from KK01 using Tris acetate; lane 4, purified material from KK01<i>pamABCDE</i> using Tris acetate; lane 5, purified material from KK01<i>ctrA</i> using Tris acetate; lane 6, purified material from KK01<i>ctrA</i> using surface PBS extraction; lane 7, purified material from KK01<i>ctrA pamABCDE</i> using Tris acetate. MS = molecular size (in kDa).</p

1-D proton NMR spectrum of the polysaccharide.

<p>The integration values show the relative molar ratio of the two polysaccharides, A_m and (B–C)_n of about 1∶3. For numbering, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075409#pone-0075409-t003" target="_blank">Table 3</a> (C3e and C3a designate the equatorial and axial proton, respectively, of the C-3 methylene group).</p

MOESM4 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

Additional file 4. Chromatographic profiles of the sugar standards in the HPAEC method. As outlined in “Methods” section, the HPAEC analyses were carried out in two separate runs using two different programs (i.e. different columns and gradients) for each sample. In bold are the sugars quantified using the respective program. (A) Program 1 was used to quantify the amounts of fucose (Fuc), rhamnose (Rha), arabinose (Ara), galactose (Gal), glucose (Glc), galacturonic acid (GalA), and glucuronic acid (GlcA) on a Dionex PA20 column eluted using a NaOH/NaOAc gradient. (B) Program 2 was used to quantify the amounts of xylose (Xyl) and mannose (Man), which eluted as one peak in program 1, on a Dionex PA1 column eluted isocratically using 2 mM NaOH