ユメ ミル ヘヤ ギソウ サレタ コクハク

Generally，　the　narrations　of　Uno　Kouji’s　novels　are　called“complicated　narrations”or　regarded　as“disordered narrations”，　In　this　article　I　deal　with　this　problem；what　makes　it　disordered　paying　my　attention　to　the　point　of　view of　the　narration．　The　present　of　the　story　and　the　present　when　the　narrator　speaks　are　sometimes　different　each　other and　sometinles　seem　to　be　the　same　in　this　novel；“YUME－MIRU　HEYA”，　This　makes　the　matter　complicated． 　Then，　a　unique　property　of　narration　of“YUME－MIRU　HEYA”is　that　using　irony，　emphasis　and　saying　impossibi1・ ity　to　express　his　experiences　so　many　times．　The　narrator　is　eager　to　show　how　strange　his　habit　is，　on　the　other　hand he　throw　showing　his　experiences　out． 　　The　narrator，　who　tells　the　readers　to　read　this　story　as　a　confession　of　his　secret　life，　thinks　that　the　belongings such　as　picture　frames，　book　shelf，　books　and　so　on　are　represent　their　owner’s　personality．　But　his　belongings　are all　imitations．　So　if　his　belongings　represent　himself，　he　is　also　an　imitation．　There’s　no‘true　life’or‘truth’　to　confess． The　last　sentence；“I　don’t　know”is　symbolic　of　this　novel’s　point．　I　think“YUME・MIRU　HEYA”is　the　novel disguised　as　a　confession，　and　it　might　has　been　a　parody　of‘WATAKUSHI　SHOUSETSU’

Über den Erfolg der Laminektomie bei spinaler Kinderlähmung

In drei Fau65llen von spinaler Kinderlähmung, bei deneu hauptsächlich Lähmung der oberen Extremitäten vorlag und die physikalisch-therapeutischen Mittel absolut versagten, hat der Verfasser auf Halsanschwellung die Laminektomie ausgeführt. Nach Ablosung des Rückenmarks von der verwachsten Umgebung, (die mit der Rückenmark verwachst), konnte Verfasser auffallend gute Erfolge beobachten. Die Motilitätsstoruugen wurde im 1. Falle innerhalb 14 Tagen, im 2. Falle 20 Tagen und im 3. Falle 8 Tageu nach der genanutem Operation beseitigt. Verfasser weist auf die gauz besouders interessaute Resultate hiu, dass in dem 1. Falle, bei dem schon 9 Mouate nach dem ersteu Auftreteu der Lähmung veistrichen waren, gleich nach der Operation bedeutende Erleichterung aufgetreten ist

The Role of the Equine Herpesvirus Type 1 (EHV-1) US3-Encoded Protein Kinase in Actin Reorganization and Nuclear Egress

The serine-threonine protein kinase encoded by US3 gene (pUS3) of alphaherpesviruses was shown to modulate actin reorganization, cell-to-cell spread, and virus egress in a number of virus species. However, the role of the US3 orthologues of equine herpesvirus type 1 and 4 (EHV-1 and EHV-4) has not yet been studied. Here, we show that US3 is not essential for virus replication in vitro. However, growth rates and plaque diameters of a US3-deleted EHV-1 and a mutant in which the catalytic active site was destroyed were significantly reduced when compared with parental and revertant viruses or a virus in which EHV-1 US3 was replaced with the corresponding EHV-4 gene. The reduced plaque sizes were consistent with accumulation of primarily enveloped virions in the perinuclear space of the US3-negative EHV-1, a phenotype that was also rescued by the EHV-4 orthologue. Furthermore, actin stress fiber disassembly was significantly more pronounced in cells infected with parental EHV-1, revertant, or the recombinant EHV-1 expressing EHV-4 US3. Finally, we observed that deletion of US3 in EHV-1 did not affect the expression of adhesion molecules on the surface of infected cells

Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency

Cyprinid herpesvirus 3 (CyHV-3), commonly known as koi herpesvirus (KHV), is a member of the Alloherpesviridae, and is a recently discovered emerging herpesvirus that is highly pathogenic for koi and common carp. Our previous study demonstrated that CyHV-3 becomes latent in peripheral white blood cells (WBC). In this study, CyHV-3 latency was further investigated in IgM⁺ WBC. The presence of the CyHV-3 genome in IgM⁺ WBC was about 20-fold greater than in IgM⁻ WBC. To determine whether CyHV-3 expressed genes during latency, transcription from all eight open reading frames (ORFs) in the terminal repeat was investigated in IgM⁺ WBC from koi with latent CyHV-3 infection. Only a spliced ORF6 transcript was found to be abundantly expressed in IgM⁺ WBC from CyHV-3 latently infected koi. The spliced ORF6 transcript was also detected in vitro during productive infection as early as 1 day postinfection. The ORF6 transcript from in vitro infection begins at -127 bp upstream of the ATG codon and ends +188 bp downstream of the stop codon, +20 bp downstream of the polyadenylation signal. The hypothetical protein of ORF6 contains a consensus sequence with homology to a conserved domain of EBNA-3B and ICP4 from Epstein-Barr virus and herpes simplex virus 1, respectively, both members of the Herpesviridae. This is the first report of latent CyHV-3 in B cells and identification of gene transcription during latency for a member of the Alloherpesviridae.This is the publisher’s final pdf. The published article is copyrighted by the American Society for Microbiology and can be found at: http://jvi.asm.org/

Patterns of cerebral glucose metabolism detected with positron emission tomography differ in multiple system atrophy and olivopontocerebellar atrophy

We used positron emission tomography with [ 18 F] fluorodeoxyglucose to study local cerebral metabolic rates for glucose (ICMRglc) in patients with multiple system atrophy (MSA), sporadic olivopontocerebellar atrophy (sOPCA), and dominantly inherited olivopontocerebellar atrophy (dOPCA) in comparison with normal control subjects. In MSA, absolute lCMRglc was significantly decreased in the brainstem, cerebellum, putamen, thalamus, and cerebral cortex. In sOPCA, absolute lCMRglc was significantly decreased in the brainstem, cerebellum, putamen, thalamus, and cerebral cortex. In dOPCA, absolute lCMRglc was significantly decreased in the brainstem and cerebellum but not in the other structures. Examination of lCMRglc normalized to the cerebral cortex in comparison with normal controls revealed in MSA significant decreases in the brainstem, cerebellum, and putamen but, in both sOPCA and dOPCA, significant decreases only in the brainstem and cerebellum. The findings indicate that these three disorders all show a marked decrease of lCMRglc in the brainstem and cerebellum but differ in the degree of hypometabolism in forebrain and cerebral cortical structures. The results are consistent with the possibility that, in many cases, sOPCA will evolve into MSA. Moreover, positron emission tomography may provide helpful diagnostic information in these neurodegenerative diseases.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50356/1/410360208_ftp.pd

Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin

X線自由電子レーザーを用いて、光照射によるチャネルロドプシンの構造変化の過程を捉えることに成功. 京都大学プレスリリース. 2021-03-26.Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to control neural activity with light . Light absorption causes retinal chromophore isomerization and subsequent protein conformational changes visualized as optically distinguished intermediates, coupled with channel opening and closing. However, the detailed molecular events underlying channel gating remain unknown. We performed time-resolved serial femtosecond crystallographic analyses of ChR by using an X-ray free electron laser, which revealed conformational changes following photoactivation. The isomerized retinal adopts a twisted conformation and shifts toward the putative internal proton donor residues, consequently inducing an outward shift of TM3, as well as a local deformation in TM7. These early conformational changes in the pore-forming helices should be the triggers that lead to opening of the ion conducting pore

ヒロツ カズオ ショキ サクヒン ニ ミル セイジツ ナ オトコ モラル ノ ナイジツ

The　early　works　of　Kazuo　Hirotsu，　which　were　published　in　1918　and　1919，“Morozaki－yuki”（1918），“Yamori” （1919）and“Nami－no－ue”（1919）are　generally　regarded　as　materials　for　his　private　life　or　his　personality．　And　they are　also　defined　as　a　branch　of　the　Naturalism　in　Japanese　hterature．　But　the　works　are　quite　similar　to　those　of　the young　novelists　belonging　to　the　Shirakaba　group　in　point　of　its　morality．　These　three　novels　of　Hirotsu　have　an outline　that　a　novelist　who　tainted　a　virgin　woman　by　his　lust　seeks　to　overcome　his　destiny　and　circumstances　by　his power　of　will，　and　makes　an　effort　to　create　good　works．　The　novelist，　who　is　the　hero　and　narrator　of　these　three works，　insists　that　once　a　man　has　love　affair　with　a　woman，　he　is　responsible　for　it　and　in　this　case‘responsibility’ mean・marryi・g　h…H・・h・uld・・t　q・it・carryi・g　hi・marri・g・with　hi・．ugly・nd・・arse　wif・n・噸t・・h・w　h・m・y suffer　pain　because　he　committed　a　sin，　He　thinks　that　to　atone　for　this　sin　is　to　lead　a　quiet　and　happy　life　with　his wife－Honesty’is　the　essential　part　of　his　morality．　And　he　seems　to　be　a　really　honest　man．　And　besides，　being　an honest　man　is　being　an　honest　novelist　because　his　anguish　as　a　man　and　as　a　novelist　are　linked　together　with　the word‘life’． 　　The　foremost　goal　of　this　treatise　is　to　prove　that　this　morality　was　not　only　the　character　of　Hirotsu　in　the　middle of　Taisho　period　by　using　materials　such　as　other　novels　of　the　Shirakaba　group　and　reputations． 　　Hirotsu’s　these　three　novels　have　the　same　rule　with　one　of　the　Shirakaba　group’s　novels　which　have　a　typical　style of　self－straining　and　overcoming　obstacles．　Such　novels　have　a　certain　chart　like　this：ahero，　who　is　an　artist，　makes an　error　or　faces　difficulties　and　overcomes　those　obstacles　with　help　from　his　wife（or　lover），and　this　experience m・k・・lt　p・ssibl・f・・hiM　t・be　a　m・n　wh・b・・b・tt・・pers・n・1ity　and　p・i・・t・lif・th・n　h・will　be　abl・t・c・eat・b・tte「 works．　Better　works　need　a　better　life，　and　a　better　life　needs　a　better　wife　with　adoration　for　her　husband．　Therefore the　heroes　expect　their　wives　to　support　them　in　the　hard　times　and　to　be　go’od　housekeepers　in　daily　life．　This　is　the inside　of　the　romantic　love　ideology　in　the　Shirakaba　group，　and　of　the　morality　of　Hirotsu’s　these　three　novels

STUDIES ON THE INFLUENCE OF HYDRAZINE, SUBSTITUTED HYDRAZINES, AND RELATED NITROGENOUS COMPOUNDS ON THE CARBOHYDRATE METABOLISM IN THE RABBIT.

DISSERTATION (PH.D.)--THE UNIVERSITY OF MICHIGANDissertation Abstracts International,Mode of access: Internet