Optimization of Steam Distillation of Essential Oil of Eucalyptus tereticornis by Response Surface Methodology

This paper deals with optimization of yield of Eucalyptus tereticornis oil in steam distillation using response surface methodology (RSM). The factors considered were mass of solute/solvent ratio (A), extraction time (B) and steam rate (C). These parameters were varied at two levels. Conditions of optimum oil yield predicted were 105 min of extraction time and 0.032 kg/h steam rate. These factors gave an optimum oil yield of 2.05 %. Significant model terms were time, steam rate, and the interactions between them. Analysis of variance (ANOVA) indicates that the model was significant as evidenced from R2 of 0.9844 and the model F-value of 49.13. The oil yield predicted by the model was closed to the experimentally determined values (1.85 % and 1.87 % respectively); hence the model can be used for prediction of oil yield in essential oil extraction from E. tereticornis leaf via steam distillation method.Keywords: RSM, Eucalyptus tereticornis, steam distillation, Box-Bohnke