Isolation and characterization of 12 polymorphic tetranucleotide microsatellite loci in the apostlebird (Struthidea cinerea)

The apostlebird (Struthidea cinerea) is an Australian endemic passerine belonging to the Corcoracidae family. The species is highly gregarious throughout the year and the name of the species refers to the apparent prevalence of social groups of around 12 birds. The species is becoming a model system for the study of sociality in vertebrates, which will require the analysis of relatedness, paternity and maternity. We characterize 12 microsatellite loci tested for polymorphism on 25 individuals from a population in western New South Wales, Australia. The number of alleles ranged from 4 to 9 per locus. Expected heterozygosities ranged from 0.69 to 0.88. This microsatellite panel will facilitate future studies that will advance our understanding of dispersal processes, inbreeding avoidance and reproductive skew in social animals. <br /

Experiences of community pharmacists involved in the delivery of a specialist asthma service in Australia

Background: The role of community pharmacists in disease state management has been mooted for some years. Despite a number of trials of disease state management services, there is scant literature into the engagement of, and with, pharmacists in such trials. This paper reports pharmacists feedback as providers of a Pharmacy Asthma Management Service (PAMS), a trial coordinated across four academic research centres in Australia in 2009. We also propose recommendations for optimal involvement of pharmacists in academic research. Methods: Feedback about the pharmacists experiences was sought via their participation in either a focus group or telephone interview (for those unable to attend their scheduled focus group) at one of three time points. A semi-structured interview guide focused discussion on the pharmacists training to provide the asthma service, their interactions with health professionals and patients as per the service protocol, and the future for this type of service. Focus groups were facilitated by two researchers, and the individual interviews were shared between three researchers, with data transcribed verbatim and analysed manually. Results: Of 93 pharmacists who provided the PAMS, 25 were involved in a focus group and seven via telephone interview. All pharmacists approached agreed to provide feedback. In general, the pharmacists engaged with both the service and research components, and embraced their roles as innovators in the trial of a new service. Some experienced challenges in the recruitment of patients into the service and the amount of research-related documentation, and collaborative patient-centred relationships with GPs require further attention. Specific service components, such as the spirometry, were well received by the pharmacists and their patients. Professional rewards included satisfaction from their enhanced practice, and pharmacists largely envisaged a future for the service. Conclusions: The PAMS provided pharmacists an opportunity to become involved in an innovative service delivery model, supported by the researchers, yet trained and empowered to implement the clinical service throughout the trial period and beyond. The balance between support and independence appeared crucial in the pharmacists engagement with the trial. Their feedback was overwhelmingly positive, while useful suggestions were identified for future academic trials

Vagal Nerve Stimulation Therapy: What Is Being Stimulated?

Vagal nerve stimulation in cardiac therapy involves delivering electrical current to the vagal sympathetic complex in patients experiencing heart failure. The therapy has shown promise but the mechanisms by which any benefit accrues is not understood. In this paper we model the response to increased levels of stimulation of individual components of the vagal sympathetic complex as a differential activation of each component in the control of heart rate. The model provides insight beyond what is available in the animal experiment in as much as allowing the simultaneous assessment of neuronal activity throughout the cardiac neural axis. The results indicate that there is sensitivity of the neural network to low level subthreshold stimulation. This leads us to propose that the chronic effects of vagal nerve stimulation therapy lie within the indirect pathways that target intrinsic cardiac local circuit neurons because they have the capacity for plasticity